2018, Volume 12, Issue 2 (Volume 12, Issue 2, August-December 2018)

Bachelors safety culture formation in the field of environmental protection
Natalya V. Kuznetsova, Andrey A. Soloviev, Lyudmila Kh. Botasheva, Igor A. Lebedev, Karina S. Sarkisyan, Vladimir I. Kurdyumov, Kirill A. Chistyakov, Alexey I. Prokopyev

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Abstract

Background: The relationship between society and nature is complex and contradictory. New generations need a different view of the environment, which is free from technocracy and aimed at preserving and multiplying natural sources. This study is aimed at the formation of competences’ system of future engineers in the field of environmental protection, contributing to the preservation of environmental relations and responsibility to future generations.
Material and Methods: Among the students participating in the pedagogical experiment a survey was conducted aimed at identifying the students’ cognitive activity and motivation while the study of the theoretical foundations of life safety; pedagogical testing for the evaluation of natural science-based, environmental, logical thinking, the method of expert assessments was used for the analysis of practical skills of students in solving occupational tasks of environmental protection, pedagogical observation, conversations in the framework of the delayed control over the occupational growth of graduates. The study uses statistical methods: calculation of descriptive statistics for the analysis of dispersion measures and grouping of pedagogical control’s indicators; Student criterion for the analysis of similarities/differences in the experimental and control groups of students.
Results: The authors identified and substantiated the structural and content characteristics of life safety culture of environmental protection bachelors. The content and technological basis and the model for activity safety culture formation are defined and proved. The criteria and indicators of life safety culture manifestation are developed, on their basis the levels of its formation (occupational-basic, occupational-adaptive, occupational-functional) are revealed.
Conclusion: As a result of the implementation of the author’s model, a positive dynamic in the formation of life safety culture was recorded among future engineers in environmental protection; the occupational and functional level of the manifestation of life safety culture significantly was increased among the students of control groups, which confirmed its effectiveness. The obtained data showed that the University stage of life safety culture formation in the aspect of environmental problems creates the Foundation for further occupational development of a specialist in the development of environmental protection and helps to reduce life risks.

Keywords: environmental protection, activity safety culture, safety, human interaction with the environment, the danger of natural, man-made, environmental and social origin

Citation: Kuznetsova NV, Soloviev AA, Botasheva LK, Lebedev IA, Sarkisyan KS, Kurdyumov VI, et al. Bachelors safety culture formation in the field of environmental protection. Eurasia J Biosci. 2018;12(2):157-66.

Student ecological consciousness as determining component of ecological-oriented activity
Elena I. Cherdymova, Svetlana A. Afanasjeva, Aleksandr G. Parkhomenko, Maria B. Ponyavina, Ekaterina S. Yulova, Irina A. Nesmeianova, Oleg A. Skutelnik

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Abstract

The state of the issue: The current type of environmental consciousness determines the behavior of people in relation to the environment. Environmental education is a part of the integral pedagogical process of any educational institution, aimed at the systematic knowledge assimilation about the environment, society, development of practical skills and environmental activities. Environmental education is aimed at the formation of environmental culture of the individual to protect the environment.
Material and Methods: The main method of research is testing, which promotes the study of environmental consciousness development level and its structural components among the students. Also in the process of research were used: methods of research and regulatory sources; socio-psychological diagnostics, psycho-diagnostic techniques; mathematical methods and methods of computer data processing.
Results: The authors identified and justified the structural and content characteristics of environmental consciousness. The data obtained during the study of the structural components of the student environmental consciousness are compared. Individual ecological consciousness is understood by the authors as a relatively stable integral mental education of the subject, which is formed in the process of environmental training, characterized by the level of environmental knowledge, attitudes, relations to nature, intentions that determine a holistic environmentally-oriented behavior aimed at protecting the environment.
Conclusions: The effective formation of ecological consciousness, in accordance with the requirements of modern society, can be when there is a designed diagnostic toolkit that allows monitoring at each stage of ecological consciousness formation. In the structure of individual ecological consciousness four structural components can be allocated: cognitive, valuable-motivational orientation, cognitive.

Keywords: ecological culture, ecological consciousness, structure of ecological consciousness, ecological attitudes

Citation: Cherdymova EI, Afanasjeva SA, Parkhomenko AG, Ponyavina MB, Yulova ES, Nesmeianova IA, et al. Student ecological consciousness as determining component of ecological-oriented activity. Eurasia J Biosci. 2018;12(2):167-4.

Socio-psychological aspects of preschoolers’ formation of ecological installation for environmental protection
Victor Y. Machnev, Ludmila Yu. Grudtsina, Dmitriy A. Pashentsev, Alfija N. Zinnatullina, Evgeniya M. Pavlenko, Kristina V. Fedorchuk, Alexandr L. Makarov, Lyudmila V. Kolomiychenko

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Abstract

Background: Overcoming of ecological crisis requires formation such human consciousness, such attitude to Nature and Human (i.e. to yourself and other people) based on awareness that human isn’t against nature. The article there is a problem between that the child must have already formed structural identity, ecological consciousness, defining nature of relationships between human and the world, it is a resource of knowledge about process of development of ecological units and its structural constituent among preschoolers as a part of ecological culture of a person.
Methods of the research: Leading method of the study of this issue is diagnostic, which allows considering this theme as a dynamic process. There are methods that was used during the process of the research: study of scientific and regulatory resources, ascertaining psycho-pedagogical experiment, analysis and synthesis of psycho-pedagogical experience, socio-psychological diagnostics: quiz, questioning, interview, observation, testing, expert review; psychodiagnostic methodologies; mathematical, statistical methods and methods of computer data processing.
Results: Consists in theoretical and experimental research of features of children ecological consciousness, character settings in preschool age. The authors identified and substantiated the ecological consciousness is considered as a core of child personality, characterizing by subjective-integrative essence: complex of new growths, common for preschool childhood, ecological aims, and ecological idea, its inner component provides further self-development of personality, and external – acts, deeds of a child in real life.
Conclusion: There are results of the study of children ecological consciousness for environmental protection settings at preschool age in urban and rural kindergarten in this work. It is necessary to base on leading structural component of ecological installation during the work with preschoolers and namely on affective. Taking this type as dominant, we will use preschoolers’ ecological attitude as structural component of ecological installation of personality. Also it is necessary to consider the fact that preschoolers are ready to active acts for environmental protection. They can’t wait to take part in different events. This desire and development of behavior component must be considered, evolving ecological setting of preschoolers and ecological consciousness at all.

Keywords: preschool age, ecological installation, ecological consciousness, ecological setting, environmental protection, types of ecological settings

Citation: Machnev VY, Grudtsina LY, Pashentsev DA, Zinnatullina AN, Pavlenko EM, Fedorchuk KV, et al. Socio-psychological aspects of preschoolers’ formation of ecological installation for environmental protection. Eurasia J Biosci. 2018;12(2):175-80.

Air pollution by road traffic and its measurement methods
Olga V. Kurnykina, Olga V. Popova, Svetlana V. Zubkova, Dmitrii V. Karpukhin, Vladimir P. Pavlov, Petr K. Varenik, Irina A. Aleshkova, Lyudmila Yu. Novitskaya

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Abstract

Background: Environmental problems of cities are associated with excessive concentration of population, transport and industrial enterprises in relatively small areas with anthropogenic landscapes formation, far from the state of ecological balance. The present study is aimed at developing recommendations for environmental measures aimed at the management of anthropogenic tendencies, forecasting the state of air pollution, carrying out the necessary environmental measures and developing a system for managing road influences on the urban environment.
Material and Methods: Assessment of air pollution and conclusions about the need to develop technical and organizationally-technical measures to reduce emissions of vehicles is based on the analysis of the concentrations of impurities obtained by calculation and instrumental methods. In the course of the study, the methods of statistical processing and analysis of measurement data were used. Toolkit for modeling the state of the atmosphere and programming allowed developing a software calculation complex that automates and visualizes the analysis tasks of the traffic flow.
Results: The authors substantiate the toolkit for assessing the environmental situation of the city and identify patterns of its formation. The intensity of traffic flow on the streets of the city – highways is defined. The regularities of pollution fields’ formation depending on the territory and intensity of traffic flows are revealed. The software to calculate the map of the city atmosphere pollution by motor transport emissions is developed.
Conclusions: The task is solved of a comprehensive assessment of air pollution degree in the city by impurities emitted from the exhaust gases of cars, separately from pollutants coming from stationary sources of pollution. The study allows predicting the effectiveness of various environmental measures related to civil construction, construction of new roads, regulation of traffic flows.

Keywords: air pollution, air emissions, computer program, concentration calculation, cars

Citation: Kurnykina OV, Popova OV, Zubkova SV, Karpukhin DV, Pavlov VP, Varenik PK, et al. Air pollution by road traffic and its measurement methods. Eurasia J Biosci. 2018;12(2):181-8.

Cultural heritage sites’ protected areas as a tool of urban environment development
Evelina P. Pecherskaya, Lyudmila V. Dzhabborova, Lyudmila V. Averina, Irina I. Firulina, Marina S. Ivankina, Diana A. Akopyan

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Abstract

One of the methods of comprehensive protection of cultural heritage objects in a certain territory includes regulation of restrictions on the territory of the cultural heritage site, protection zones, town planning regulations in these zones. The authors analyzed legislative gaps and contradictions that arise in determining the legal regime of territories occupied by cultural heritage sites and adjacent territories. The methodology for determining the protection zone in Latvia and Russia had been paid special attention. The statistics of both counties on the issue at stake is investigated. Map data on the issue is given. The ways of solving the identified problems on the example of environmental enforcement are suggested. The research results are summarized and assessed from legal, economical and technical points of view and thus allow the processing and generalization of the acquired knowledge for other future similar solutions and better understanding of post-Soviet regulation differences on the matter.

Keywords: protected areas, cultural heritage site, urban environment, environmental defense, special-use areas

Citation: Pecherskaya EP, Dzhabborova LV, Averina LV, Firulina II, Ivankina MS, Akopyan DA. Cultural heritage sites’ protected areas as a tool of urban environment development. Eurasia J Biosci. 2018;12(2):189-4.

Screening of bacteria for antifungal activity against phytopathogenic fungi of sugarcane (Saccharum officinarum L.)
K. Girish

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Abstract

Colletotrichum falcatum and Fusarium moniliforme are the causal agents of red-rot and pokkah boeng disease in sugarcane respectively, which are among the most destructive diseases in this crop worldwide. In this work, antifungal property of bacteria was evaluated against these two plant pathogenic fungi. Initially six bacterial strains were screened by dual culture technique and two strains Bacillus cereus MTCC 9017 and Pseudomonas monteilii MTCC 9796 were selected for further studies. Ethyl acetate extracts of culture filtrates of these two bacteria were obtained and assayed for antifungal potential by poisoned food method. Both of the bacterial isolates tested showed significant antifungal activity against Colletotrichum falcatum and Fusarium moniliforme. The results of this study indicate the possibility of further exploring B. cereus MTCC 9017 and P. monteilii MTCC 9796 as potential biocontrol agents.

Keywords: Colletotrichum falcatum, Fusarium moniliforme, Bacillus cereus, Pseudomonas monteilii, biocontrol

Citation: Girish K. Screening of bacteria for antifungal activity against phytopathogenic fungi of sugarcane (Saccharum officinarum L.). Eurasia J Biosci. 2018;12(2):195-203.

A research approach supporting micropropagation and domestication of blueberry (Vaccinium corymbosum L.) in Egypt
Gamil Rayan Abuo El-dis Mohamed, Bautista Hugo, Khusnetdinova Landysh Zavdetovna, Timofeeva Olga Arnoldovna

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Abstract

The northern high bush blueberry, Vaccinium corymbosum L., is a North American species of blueberry, which has become a food crop of economic importance around the world. In the recent years, there has been an extended commercial interest for many wild species of Vaccinium products in Israel, Japan and New Zealand and of course Egypt. Nowadays a growing interest from NGOs and various research groups. They have participated in a concerted research action with cross counter disciplinary cooperation between plant physiology, population genetics, tree breeding, food science, and socioeconomics. Studies have been initiated in several countries on geographic variation of growth, adaptive and phenological traits. Unfortunately, until now, in Egypt we do not have enough studies to establishment of international provenance series, seed banks and studies of gene flow and phylogeographic variation of Vaccinium species. This paper presents part of our methods and reproducible protocols for clone propagation. The accumulated knowledge will be applied for a domestication strategy of Vaccinium corymbosum L. in Egypt. In addition, the outcome from this study will be guidelines for propagation and management of the germplasm of other Vaccinium species and resources.

Keywords: Domestication of blueberry, Food security, plant breeding, micropropagation, Vaccinium corymbosum L.

Citation: Mohamed GRAE, Hugo B, Zavdetovna KL, Arnoldovna TO. A research approach supporting micropropagation and domestication of blueberry (Vaccinium corymbosum L.) in Egypt. Eurasia J Biosci. 2018;12(2):205-10.

Monitoring of urban environmental quality using chlorophyll delayed fluorescence
Guzel R. Valeeva, Mikhail V. Karpov, Zarina M. Khafizova, Violetta A. Gabdrakhimova

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Abstract

This article presents the measurements of delayed fluorescence from Pinus sylvestris leaves against the functional load on the urban territory. We compare the values obtained with the results of chemical and analytical studies of the soil quality. The sampling was carried out according to a spatial grid designed on the basis of the functional load on the territory. The bioindication method as well as chemical and analytical tests of plant and soil samples taken at each site under study allow to differentiate these locations according to their ecological conditions. We made attempts to assess the degree of influence of various factors on some indicators of plant vitality. Calculations of the correlation coefficient between the content of a number of components in the soil and bioindication data suggest that the photosynthetic activity of plants in urban conditions is more dependent on the state of the atmosphere.

Keywords: chlorophyll, delayed fluorescence, environmental monitoring, pollution, bioindicator, pine

Citation: Valeeva GR, Karpov MV, Khafizova ZM, Gabdrakhimova VA. Monitoring of urban environmental quality using chlorophyll delayed fluorescence. Eurasia J Biosci. 2018;12(2):211-7.

Artemisia dracunculus in combination with chitosan nanoparticle biofilm improves wound healing in MRSA infected excisional wounds: An animal model study
Reza Ranjbar, Alireza Yousefi

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Abstract

The objective of the present study was to assess effect of Artemisia dracunculus in combination with chitosan nanoparticle biofilm on MRSA infected excisional wounds. Thirty rats were randomized into five groups of six rats each. Group I: Animals with uninfected wounds treated with 0.9% saline solution. Group II: Animals with infected wounds treated with saline. Group III: Animals with infected wounds were dressed with chitosan nanoparticle biofilm. Group IV: Animals with infected wounds were treated topically with Artemisia dracunculus and Group V: Animals with infected wounds were treated topically with Artemisia dracunculus and dressed with chitosan nanoparticle biofilm. Wound size was measured on 6, 9, 12, 15, 18 and 21days after surgery. Microbiology, reduction in wound area and hydroxyproline contents indicated that there was significant difference (P < 0.05) between group V and other groups. Quantitative histological studies and mean rank of the qualitative studies demonstrated that there was significant difference (P < 0.05) between group V and other groups. It was concluded that the Artemisia dracunculus with chitosan nanoparticle biofilm had a reproducible wound healing potential and hereby justified its use in practice.

Keywords: Artemisia dracunculus, chitosan nanoparticle biofilm, MRSA, wound, rat

Citation: Ranjbar R, Yousefi A. Artemisia dracunculus in combination with chitosan nanoparticle biofilm improves wound healing in MRSA infected excisional wounds: An animal model study. Eurasia J Biosci. 2018;12(2):219-26.

Floristic characterization of the territory adjacent to the “Gray Heron Colony” protected area
Galina V. Demina, Adel R. Zakirov

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Abstract

The natural monument “Gray heron colony” is a specially protected area of about 100 ha with the largest colony of gray herons registered in Tatarstan Republic (Russia). The territory of the natural monument is closed to visitors. Here, we have studied the flora of the higher vascular plants of the area adjacent to the “Gray heron colony”, which is characterized by similar landscape and environmental conditions. Two main methods to identify plant species were used: fixed sites and a route method, for which a route network covering all the main biotopes of the studied area was laid. The structure of the flora was studied by analyzing systematic, geographic, biomorphological, and biocenotic compositions. The identified spectrum of the leading families (Asteraceae, Fabaceae, Rosaceae, Caryophyllaceae, Poaceae, Scrophulariaceae, Apiaceae, Ranunculaceae, Lamiaceae, and Cyperaceae), was typical for the Holarctic floristic kingdom. The spectrum of life forms found (according to Raunkiær and Serebryakov) was generally characteristic to the temperate zone: more than half of the species (57.5%) were represented by hemicryptophytes (according to Raunkiær); 67% of the species were perennials (according to Serebryakov). By biotopic localization, most species were forest and forest edge plants (31.4%). The geographical analysis has shown that most species of flora have a Euro-Asian type of distribution. Among identified species, two are listed in the Red Book of Tatarstan - Daphne mezereum L. (rare species status) and Potentilla erecta Raeusch (species reducing the number), thus being objects of special protection. This study contributes to more complete inventory of the flora of Tatarstan, Russia.

Keywords: flora, flora structure, species diversity, protected area, natural monument

Citation: Demina GV, Zakirov AR. Floristic characterization of the territory adjacent to the “Gray Heron Colony” protected area. Eurasia J Biosci. 2018;12(2):227-31.

The Effect of Saffron Alcoholic Extract on the Change in Inflammatory Proteins TNF-α and IL-6 Levels in an Experimental Model of Healthy Rats in Contrast to Diabetic Rats
Zangoei Dovvom Alireza, Khatamsaaz Saeed, Foadoddini Mohsen, Mokhtari Mohammad Javad, Zangoei Dovvom Samane

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Saffron is an important medicinal herb playing a role in treating diabetes and alleviation of hepatic malfunctioning. The present study investigates the protective effects of long-term oral use of saffron on the inflammatory proteins TNF-α and IL-6 levels in the livers of diabetic rats. Forty two mature male Wistar rats were assigned to 6 groups, namely a control group, a diabetes-induced evidence group (administered with streptozotocin for 70mg/kg.bw, ip), a healthy group administered with a low dosage of saffron, 50mg/kg.bw, another healthy group administered with a high dosage of saffron, 100mg/kg.bw, a diabetic group administered with a low dosage of saffron, 50mg/kg.bw, and another diabetic group administered with a high dosage of saffron, 100mg/kg.bw, all of which were subjected to studies for a period of two months. The animals’ serum glucose level and body weights were seminally measured followed by the use of ELISA method for quantitative evaluation of inflammatory proteins TNF-α and IL-6 levels that are known to have effects on the hepatic disorders in the liver tissue. Saffron consumption caused significant reduction in the blood glucose levels of the diabetic rats, especially in 100mg/kg.bw dosage. In the meantime, alcoholic extract of saffron was found increasing IL-6 level in diabetic and healthy groups administered with low and high dosages of saffron as compared to those in control group. Moreover, the alcoholic saffron extract was proved increasing the TNF-α level in diabetic and healthy groups administered with low and high dosages of saffron in contrast to those in control group. On the other hand, the application of low or high dosage of saffron on diabetic rats could bring about reductions in IL-6 and TNF-α levels. It can be concluded that oral use of low or high dosages of saffron for two months has provided for the improvement of the inflammatory proteins levels in the hepatic tissue of the rats with diabetes so it can be used to mitigate the adverse symptoms resulting from diabetes.

Keywords: alcoholic saffron extract, rat, diabetes, glucose, tissue protein level, TNF-α, IL-6

Citation: Alireza ZD, Saeed K, Mohsen F, Javad MM, Samane ZD. The Effect of Saffron Alcoholic Extract on the Change in Inflammatory Proteins TNF-α and IL-6 Levels in an Experimental Model of Healthy Rats in Contrast to Diabetic Rats. Eurasia J Biosci. 2018;12(2):233-8.

Biocompatibility study of composite for facial skeletal reconstruction during an experiment
I. V. Reshetov, M. E. Gaponov, K. M. Ruban, S. A. Bogoslovsky, Y. P. Zesin, R. S. Izimov, N. V. Kharkova, D. C. Svyatoslavov

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Abstract

Facial reconstruction is one of the most important issues in modern reconstructive surgery. Some defects can’t be removed using a simple surgical intervention and the use of allo- and autografts is frequently associated with complications such as tissue incompatibility or excessive traumatization of the donor site [14, 15]. The issue is urgent as development of medical technologies, material sciences and biochemistry makes it possible to create and apply modern bioconstructions. Their properties most closely approximate the bone tissue; they have both osteoconductive and osteoinductive properties. The article is aimed at the study of biocompatibility of prototypes of composite with hydroxyapatite and methyl methacrylate for their subsequent use of implants in facial reconstruction. The leading approach to the study was to examine biocompatibility of implants in 15 mature rats divided into 3 groups depending on the composition and used endoprosthesis. The ratio between methyl methacrylate and hydroxyapatite in the implant was 70 to 30 in the first group; 50 to 50 in the second group, and 30 to 70 in the third group. The endoprostheses were placed over the mandible under an experiment. The study demonstrated that the ratio of 30:70 (methyl methacrylate/hydroxyapatite) was the optimal composition for the best mechanical and biological features. No adverse reactions were found in the animals of the studied group. The article can be useful for the reconstruction with hydroxyapatite and methyl methacrylate implants to replace the facial defects such as defects of the orbit, palate, calvaria, jaw, etc.

Keywords: hydroxyapatite, methyl methacrylate, mandible, endoprosthesis, implants, reconstructive surgery

Citation: Reshetov IV, Gaponov ME, Ruban KM, Bogoslovsky SA, Zesin YP, Izimov RS, et al. Biocompatibility study of composite for facial skeletal reconstruction during an experiment. Eurasia J Biosci. 2018;12(2):239-43.

Antifungal activity of Lantana camara L., rhizosphere bacteria
K. Girish, B. M. Bhavya

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Abstract

Rhizosphere bacteria were isolated from the rhizosphere of Lantana camara L., named LcRB1, LcRB2, LcRB3 and LcRB4, and tested for antifungal activity against seed-borne fungi such as Alternaria sp., Aspergillus niger, Cladosporium sp., Curvularia sp., and Fusarium sp., by dual culture method. Rhizosphere bacteria LcRB2, LcRB3 and LcRB4 showed significant antifungal activity against all the fungi tested, while LcRB1 failed to exhibit antifungal activity against A. niger and Fusarium sp., but inhibited the growth of other fungi tested. These bacteria could be employed for biocontrol of the seed-borne phytopathogenic fungi.

Keywords: biocontrol, Lantana camara, rhizosphere bacteria, seed-borne fungi

Citation: Girish K, Bhavya BM. Antifungal activity of Lantana camara L., rhizosphere bacteria. Eurasia J Biosci. 2018;12(2):245-1.

Field evidence of the gametogenic maturation and embryonic development of the sand crab, Emerita taiwanesis: Implications for the understanding of the basis of the reproductive biology
Sinlapachai Senarat, Suchanaat Biaklai, Jes Kettratad, Somrudee Meprasert Jitpraphai, Koraon Wongkamhaeng, Woranop Sukparangsi, Chanyut Sudtongkong, Lamai Thongboon

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Abstract

Background: The reproductive histology in the decapods have been widely documented; however, this information has never been reported in Emerita taiwanesis, one of profitable species for aquaculture business in Southeast Asia.
Material and Methods: The gametogenic maturation and embryonic development of sand crabs E. taiwanesis with carapace widths of 4-5 cm (n = 20 for each sex) from Sangchan Beach, Thailand were examined by histological techniques and light microscopy.
Results: The gonadal structure was located underneath the carapace at dorsal area of the body. Under histological techniques, the ovarian maturation was observed to be enclosed with a thin layer of ovarian wall. The different phases of developing oocytes could be classified into five stages: 1) Oc1 (previtellogenic stage), 2) Oc2 (early vitellogenic stages), 3) Oc3 (late vitellogenic stage), Oc4 (mature oocyte) and Oc5 (atretic oocyte). The spermatogenic maturation occurred at the same time as female ovarian maturation. The spermatogenic stage was clearly divided into five stages: spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids, and spermatozoa. Additionally, different timing of its embryonic development was observed, and the embryos could be categorized into three major stages: 1) early development (cleavage, blastula, and gastrula), 2) early organogenesis (post-gastrula) and 3) late organogenesis.
Conclusion: In this study, histological observation of oogenesis and spermatogenesis as well as embryogenesis of sand crab E. taiwanesis highlights the conserved pattern of gametogenic maturation among decapod, which can lead to understanding of the reproductive cycle, physiology, and aquaculture strategies of this economically important crustacean in the Gulf of Thailand.

Keywords: sand carb, oogenesis, spermatogenesis, embryogenesis, Thailand

Citation: Senarat S, Biaklai S, Kettratad J, Jitpraphai SM, Wongkamhaeng K, Sukparangsi W, et al. Field evidence of the gametogenic maturation and embryonic development of the sand crab, Emerita taiwanesis: Implications for the understanding of the basis of the reproductive biology. Eurasia J Biosci. 2018;12(2):253-62.

The role of environmental innovations in ensuring sustainable development of regional entities in Russian Federation
Natalya Yu. Arefyeva

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Abstract

The article presents the interpretation of the content and structure of environmental innovations (eco-innovations) using the epistemological potential of alternative economic schools and the interdisciplinary approach, the features of eco-innovations and their dynamics in the Russian economy are analyzed. The evolution of the concept of the role of the environmental factor in the development of territorial entities is analyzed, with special attention paid to the analysis of theories of regional economy and regional development, the economics of nature management, the theory of sustainable development and the ecologization of economic relations. The definition of eco-innovations as a new product or process representing a certain consumer and economic value is given, but at the same time it significantly reduces the negative impact on the environment in comparison with the existing alternatives. The thesis about the increasing role of eco-innovations in ensuring a stable trajectory of translational dynamics is formulated. It is proved that the low level of ecologization of economic relations is the result of the ineffectiveness of state regulation measures aimed at increasing the ecological component of added value, which impedes the improvement of the quality of life of the population and limits the growth of aggregate expenditures. The set of indicators of eco-innovation development is substantiated and presented using the tabular method of their dynamics in the period from 2013 to 2016. In the process of production of goods, works and services in the Russian Federation. The provisions formulated in the course of the study prove the need to develop and implement programs aimed at stimulating the processes of greening and innovation of economic relations. The recommendations aimed at the formation of eco-innovations in the regional economy and the use of their potential for the implementation of strategic guidelines for the development of the subjects of the Russian Federation are suggested.

Keywords: regional economy, sustainable regional development, the process of ecologicalization of economic relations, the ecological potential of the territory, eco-innovation, environmental entrepreneurship, barriers to the introduction of eco-innovations, regional programs for socio-economic development

Citation: Arefyeva NY. The role of environmental innovations in ensuring sustainable development of regional entities in Russian Federation. Eurasia J Biosci. 2018;12(2):263-9.

Synthesis of magnetic adsorbents for the purification of aquatic environments from oil
Tatiana R. Denisova, Irina Y. Sippel, Yury K. Rubanov, Yulia E. Tokach, Sergey Yu. Zenin

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Abstract

The technology of producing an adsorbent, which have magnetic properties, for the oil removal from waste water and surface water bodies by the controlled magnetic field, is proposed. A method of the magnetic adsorbent producing includes the mixing of magnetite and the absorbent material which is degradable steel slag. The weight ratio of slag: magnetite = 1 : ( 1.5-2.0). Optimal particle size of the components is 70-100 microns. Application of a magnetic treatment with the treated water prior acidification to pH = 3 ÷ 4 improves the efficiency of oil removal from waste water up to 1.5 times. To remove spills of petroleum products by the proposed magnetic adsorbent we developed diagram of the device, which is mounted on board a catamaran – type oil skimmers. The device includes a body, which is open to the entry and exit of treated water, the bottom of which is made in the form of endless moving belt. Above the tape at the end of its movement is set oil-gathering magnetic drum system. Adding oil adsorbent layer is made from a hopper disposed at the front of the vessel and provided with a drum feeder which provides a predetermined flow of the adsorbent depending on the thickness of the oil layer. Due to the high bulk density is a quick dip in the adsorbent layer of mineral oil. Large flocs, lost buoyancy deposited onto a moving belt and move to the oil-gathering drum. Removal of saturated adsorbent is carried out with the drum surface scraper, passing into a trough in which is located a rotating auger.

Keywords: magnetic adsorbent, magnetic treatment, magnetic field, petroleum products, structural changes, magnetite, steel slag

Citation: Denisova TR, Sippel IY, Rubanov YK, Tokach YE, Zenin SY. Synthesis of magnetic adsorbents for the purification of aquatic environments from oil. Eurasia J Biosci. 2018;12(2):271-5.

Experimental findings in forest soil mechanics
Mikhail Grigorev, Alexandra Grigoreva, Igor Grigorev, Olga Kunickaya, Daria Stepanova, Margarita Savvinova, Mikhail Sidorov, Ekaterina Tomashevskaya, Irina Burtseva, Olga Zakharova

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Abstract

Forest is a source of natural resources that are essential for social production and human welfare. It is a running gear in the mechanism of biosphere maintenance, housing the greatest amount of sun energy and living material. It is one of the sources of oxygen on earth. When logging causes significant damage to forest soils, regeneration is not the only what is disturbed. In fact, the productivity of second-growth forest will fall, the hydrologic status of soil under disturbed forest will drop, and the overall forest landscape will change. Such after-effects are long-standing, and will be reminding of themselves for over several decades after logging. The principles of sustainable forest management are outlined so that stress the necessity of keeping forest soils undisturbed when using forestland. Our article deals with physical and mechanical properties of forest soil and subsoil, determines what way of using them is the most meaningful, and what possibilities are to take in order to reduce the risk of soil damage. A series of lab experiments were held to achieve this goal. The procedures were guided by GOST 5180-84. For analysis, 10 soil samples were collected. Research resulted in data on the effect that forest machines have on soil. Research results can be used to plan logging process that would be more friendly to the environment.

Keywords: forest soil/subsoil, logging operations, soil damage, forest machines, environmental safety and compliance

Citation: Grigorev M, Grigoreva A, Grigorev I, Kunickaya O, Stepanova D, Savvinova M, et al. Experimental findings in forest soil mechanics. Eurasia J Biosci. 2018;12(2):277-87.

Evaluation of the Balneological properties of Sapropels Lacustrine deposits of the Surgut District (Khanty-Mansiysk autonomous Okrug – Yugra, Russia)
I. V. Kravtsov, K. Yu. Kudrin

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Abstract

A large-scale study of the lakes of Khanty-Mansiysk Autonomous okrug –Yugraof the sapropelic mud: more than 400 lakes have been surveyed throughout the district. The suitability of the sapropelic sediments of lakes has been established for use in the medical purposes at various diseases. The main regularities of geomorphological placement and spatial distribution of lakes with therapeutic muds have been updated. Estimated inferred resources of therapeutic mud are significant and able to satisfy not only domestic needs of the district. Allocated promising areas allow the use of existing transport infrastructure for the development of the occurrence of therapeutic mud.

Keywords: Western Siberia, Khanty-Mansiysk, Autonomous okrug – Yugra, II terrace above flood-plain, lakes, sapropel, balneological characteristics

Citation: Kravtsov IV, Kudrin KY. Evaluation of the Balneological properties of Sapropels Lacustrine deposits of the Surgut District (Khanty-Mansiysk autonomous Okrug – Yugra, Russia). Eurasia J Biosci. 2018;12(2):289-5.

Air pollution investigation by organic compounds in the city
Ilnar A. Nasyrov, Gennady V. Mavrin, Munir N. Miftahov

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Abstract

In this paper carry out assessment of level of impurity of atmospheric air in the city is executed by light organic compounds. The nature of change of content of these ingredients in atmospheric air in the direction of their possible source is studied. The composition of the organic substances which are present at atmospheric air is investigated. Sampling of air on these components is made. The analysis on the gas chromatograph and processing of the received results is made. Calculation of quantitative content of light organic compounds in tests of atmospheric air is made. The character of a gradient of concentration of light organic compounds towards the city of Nizhnekamsk is defined. For the purpose of assessment of level of impurity of atmospheric air the complex index of impurity of the atmosphere for 23 trial platforms is determined by five priority volatile organic compounds. Offers on decrease in content of light organic compounds in atmospheric air are developed.

Keywords: atmospheric air, pollution, organic compounds, impurity

Citation: Nasyrov IA, Mavrin GV, Miftahov MN. Air pollution investigation by organic compounds in the city. Eurasia J Biosci. 2018;12(2):297-301.

Comparative analysis of the effect of mesenchymal stem cells on viability of autologous fat transplants by histologic examination of resorption, fibrosis, volume decrease and revascularization of fat grafts: in vivo experiment
O. I. Starceva, D. V. Melnikov, A. L. Istranov, A. V. Lundup, M. E. Kreshennikov, A. B. Shehter, G. E. Dashtoyan, M. Y. Sinelnikov, A. S. Zaharenko, K. A. Kirillova

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Abstract

Background: Autotransplantation of adipose tissue have become one of the most popular subjects for scientific researches in the field of plastic and reconstructive surgery. It has gained wide recognition and nowadays became the standard procedure for increasing volume or filling defects of soft tissues caused by trauma or processes of aging.  But despite all advantages of this technique it also has an essential shortcoming – partial necrosis with progressive resorption of transplanted adipose tissue. Achievements in cellular technologies have allowed to look at the problem of resorption from different angle.
Objective: The authors compare autologous fat transplantation to adipose-derived stem cell–enriched lipografts.
Methods: A group of 30 experimental rabbit models were included in this study and randomly divided into two groups. Fifteen models were treated with autologous fat transplantation, and the remaining fifteen were treated with adipose-derived stem cell–enriched lipografts. Further results have been estimated at 1, 3 and 6 months after the initial surgical procedure.
Results: Analysis of the survival rates of autotransplanted adipose tissue at stages 1, 3, 6 months were much higher in groups where grafts were enriched with stem cells. 
Conclusions: Adipose derived stem cell enriched lipografts have shown better angiogenetic and survival potential comparing to standard procedure. We assume that ASCs differentiated into vascular endothelial cells and contributed to neoangiogenesis in the acute phase of transplantation. These findings may partly explain why transplanted aspirated fat does not survive well and suggest clinical potential of the ASCs method for soft tissue augmentation. Further long-term studies are necessary to confirm the favorable results seen in this study.

Keywords: adipose-derived stem cells, ADSC, stem cells, Mesenchymal stromal/stem cells, Lipopreservation autologous fat transfer, fat transplantation, cell- enhanced fat grafting, rabbits, soft tissue augmentation, soft tissue filler

Citation: Starceva OI, Melnikov DV, Istranov AL, Lundup AV, Kreshennikov ME, Shehter AB, et al. Comparative analysis of the effect of mesenchymal stem cells on viability of autologous fat transplants by histologic examination of resorption, fibrosis, volume decrease and revascularization of fat grafts: in vivo experiment. Eurasia J Biosci. 2018;12(2):303-12.

Sustainable strategy of charcoal (Panglong Arang) management in the Bengkalis Regency
Muhammad Genta, Usman M. Tang, Khairul Anwar, Sri Wahyuni, Syahril Syahril

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Abstract

Background and Purpose: Charcoal is a residue that occurs from the results of decomposition of wood due to heat when most of the chemical components are carbon. One method of making wood charcoal is to use a stove. Charcoal is an important factor in determining the sustainability of the lives of surrounding communities that have long been running. Therefore the purpose of this study is to create a strategy based on the SWOT analysis to see if the charcoal business using mangrove forests can proceed or not.
Materials and Methods: Analysis of sustainable development strategies using SWOT Analysis which is one of the analyses used to formulate a strategy in an area of ​​development. This analysis consists of 4 basic elements, namely strengths, weaknesses, opportunities and threats. These four elements come from two main factors: internal factors (strengths and weaknesses) and external factors (opportunities and threats). While the stages in the SWOT analysis.
Results: Analysis of public perception of the existence of charcoal trading business in the Bengkalis District gave a “Good response” with a score of 2.66. These results illustrate that the existence of a charcoal trading business does not provide a bad impact as long as it is in a location around a mangrove forest. The results obtained in the SWOT matrix diagram are in the fourth quadrant, show that the priority of the strategy is the Strength (S) and Threats (T) strategies. The components in these two strategies need to get more emphasis and attention so that the existence of a charcoal trading business can be sustainable.
Conclusion: The public perception of the charcoal long-standing regulation in the “Bengkalis Regency” is in the “Good category” which is a prerequisite in the development of continuous charcoal. The strategy for sustainable charcoal development is in quadrant IV, namely the S-T strategy.

Keywords: Panglong Arang, SWOT, sustainable

Citation: Genta M, Tang UM, Anwar K, Wahyuni S, Syahril S. Sustainable strategy of charcoal (Panglong Arang) management in the Bengkalis Regency. Eurasia J Biosci. 2018;12(2):313-20.

Altitudinal morphogenetic traits variations of rhododendrons
Sanjay Kumar, N. Abenthung Kitahn, N. S. Jamir

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Abstract

The quantitative trait variations such as phenotypic variation (VP), genotypic variation (VG), environmental variation (VE), variation due to interaction of genotype and environment (VG×E) with genotypic and phenotypic coefficient of variation (GCV and PCV) were studied among the Rhododendrons of Wokha, Kiphiri and Kohima district of Nagaland at an altitude of (1780, 1802, 1952 masl), (3096, 3112, 3430 masl) and (1653, 2050, 2284 masl) respectively. The maximum environmental variation was recorded in the trait leaf breadth (0.956) followed by plant height (0.834) and leaf length (0.712) at an altitude of 1802 and 1780 masl of Wokha district respectively. The high variations were recorded for the traits petiole length (0.979) and petal length (0.928) due to genotype at 3430 masl, node length (0.914) due to genotype and environment interaction at 3112 masl and leaf breadth (0.862) due to environmental variation at 3096 masl of Kiphiri district respectively. The trait leaf length (0.930 and 0.874) at 1653 masl and 2050 masl varied phenotypically and petal length (0.906) environmentally at 2284 masl of Kohima district respectively.

Keywords: rhododendrons, altitude, morphogenetic variation, phenotypic variation, genotypic variation

Citation: Kumar S, Kitahn NA, Jamir NS. Altitudinal morphogenetic traits variations of rhododendrons. Eurasia J Biosci. 2018;12(2):321-4.

Specific aspects of the formation of Miscanthus planting material depending on cultivation conditions
Volodymyr A. Doronin, Viktoriya V. Dryha, Lesia M. Karpuk, Sergiy P. Vachniy, Andriy A. Pavlichenko, Valeriy P. Mykolayko, Valentyn V. Polischuk

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The results of studying some peculiar features of the formation of planting materials depending on the application of a set of technology elements – planting terms and absorbent granules and gel – are presented in the paper. It was found out that emergence terms depended on weather conditions in the years of the research. The higher the average circadian air temperature was the more intensive miscanthus emergence. It was established that the engrafting with rhizome, the intensity of sprout formation, the increase of rhizome mass, and in turn, the output of planting materials depended on planting terms, use of absorbent and weather conditions in the years of the research.

Keywords: engrafting with rhizome, absorbent, planting term, sprouts, rhizome mass

Citation: Doronin VA, Dryha VV, Karpuk LM, Vachniy SP, Pavlichenko AA, Mykolayko VP, et al. Specific aspects of the formation of Miscanthus planting material depending on cultivation conditions. Eurasia J Biosci. 2018;12(2):325-31.

Copper and cadmium-induced toxicity on the cyanobacterium Nostoc muscorum Meg 1: a comparative study
Rabbul Ibne A. Ahad, Mayashree B. Syiem

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Abstract

Seven days exposure to 10 μM Cu showed reduction in growth in terms of chlorophyll a and protein concentration by 38% and 21% in Nostoc muscorum Meg 1. Same period of exposure to 10 μM Cd recorded reduction in these two parameters by 89% and 71%. Photosynthetic pigments carotenoids, phycocyanin, allophycocyanin and phycoerythrin were compromised > 75% in Cd as against ~ 14-21% in Cu. Nitrogenase and glutamine synthetase activities were down by ~ 75% in Cd, whereas the adverse effect of Cu was ~ 13%. The photosystem II activity and respiration were reduced by 44% and 53% in Cu but toxicity was more pronounced (> 70%) in Cd. Energy dispersion X-ray analysis established Cu and Cd binding to cell surface and Fourier Transform Infrared spectroscopic analysis determined the involvement of hydroxyl, carboxylic, carbonyl, nitro and amino groups in metal binding. Langmuir isotherm study established higher metal sorption capability with Qmax of 69.7 mg/g of biomass for Cd against 17.4 mg/g of biomass for Cu. Bright Field and Scanning Electron microscopy showed severity of Cd on filaments morphology compared to Cu. The cyanobacterium showed high efficiency (> 90%) for binding both metals making it a potential organism for research in the field of bioremediation.

Keywords: Nostoc muscorum Meg 1, EDX-SEM, FTIR, Langmuir isotherm, metal removal and toxicity, Nitrogen metabolism

Citation: Ahad RIA, Syiem MB. Copper and cadmium-induced toxicity on the cyanobacterium Nostoc muscorum Meg 1: a comparative study. Eurasia J Biosci. 2018;12(2):333-45.

Influence of agrotechnical and chemical measures on weediness in sweet Sorghum crops (Sorghum Bicolor) and the output of biogas
Mykola Grabovskyi, Yurii Fedoruk, Liudmyla Pravdyva, Tetiana Grabovska, Vasil Kurylo, Natalia Fedoruk

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Abstract

Background: The use of agrotechnical and chemical measures for the protection of sweet sorghum crops from weeds improves plant growth conditions, eliminates the competition of weeds, and increases the yield of green and dry mass and the estimated output of biogas.
Materials and Methods: The field data were obtained in 2014–2016 at the research field of Bila Tserkva National Agrarian University, Ukraine. Soil cultivation, application of soil herbicide S-metolachlor (1.8·lha-1), post-emergence herbicide Prosulfuron (0.02 kg·ha-1) were studied. The trial was randomized in a 3-fold repeat.
Results: The effectiveness of the herbicide S-metolachlor application was 56.6–57.7% and the herbicide Prosulfuron – 60.1–60.8%. Using mechanical cultivation destroyed 79.6–80.3% of weeds. Maximum yield of green and dry mass and output of biogas were obtained from the Dovista hybrid in the application of Prosulfuron (69.1 t·ha-1, 16.5 t·ha-1, 6.74 thsd m3·ha-1).
Conclusions: The use of agrotechnical and chemical measures for the protection of weeds in sweet sorghum crops contributes to a significant improvement in plant growth, eliminates the competition of weeds and significantly reduces their number. The using only mechanical soil cultivation is less effective, which reduces yields of green and dry mass and the estimated output of biogas, compared with chemical measures.

Keywords: biogas, green mass, herbicide, mechanical cultivation, weed

Citation: Grabovskyi M, Fedoruk Y, Pravdyva L, Grabovska T, Kurylo V, Fedoruk N. Influence of agrotechnical and chemical measures on weediness in sweet Sorghum crops (Sorghum Bicolor) and the output of biogas. Eurasia J Biosci. 2018;12(2):347-53.

Phytocenotic characteristics and stocks of the main medicinal plants of the South-Western Altai (East Kazakhstan)
Serik A. Kubentayev, Abdrakhman N. Suleimenov, Jurii A. Kotukhov, Alevtina N. Danilova, Aidar A. Sumbembayev

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Abstract

In this paper, we review the raw stocks of medicinal plants in the South-Western Altai of the Kazakhstan part of the Altai mountainous country. During the work, we used the classic conventional techniques for the study of resources, morphology of plants and coenopopulations. In all communities, the ecological and biological and phytocenotic characteristics of species, the operational reserves of medicinal raw materials and the annual volumes of possible storage are determined. A map scheme of the point areas of distribution of the studied medicinal plants was compiled. Based on the results of research in the South-Western Altai, the Ivanovsky, Ubinsky and Western Listvyaga Ranges, distribution and inventory of 37 coenopopulations of 16 valuable medicinal plants was detected. It has been established that 8 types of MP in 21 (56%) coenopopulations refer to category I with a stock of raw materials of 10 tons or more. 9 species in 15 (41%) coenopopulations have stocks of raw materials from 1 to 10 tons (category II). 1 species in one (3%) of the population with a stock of up to 1 ton is classified in category III. Significant amount of raw materials (more than 100 tons) are formed from: Veratrum lobelianum with EZ – 1972.7 t, Stemmacantha chartamoides – 511.81 t and Chamaenerion angustifolium from common EZ – 232.2 t. Out of the examined medicinal plants, 11 (69 %) of the species are pharmacopoeial and 5 (31 %) are actively used in folk medicine. Taxonomic analysis has shown that the largest number of medicinal plants is found in families: Asteraceae Dumort. – 5 (31%) species, Fabaceae Juss. – 2 (13 %) species, Ranunculaceae Jus – 2 (13%) species.

Keywords: South-Western Altai, East Kazakhstan, resources, stocks of raw materials, vegetation, medicinal plants, industrial thickets

Citation: Kubentayev SA, Suleimenov AN, Kotukhov JA, Danilova AN, Sumbembayev AA. Phytocenotic characteristics and stocks of the main medicinal plants of the South-Western Altai (East Kazakhstan). Eurasia J Biosci. 2018;12(2):355-68.

The increased gamma-aminobutyric acid content by optimizing fermentation conditions of bacteria from kimchi and investigation of its biological activities
Vo Thanh Sang, Le Phuong Uyen, Ngo Dai Hung

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Abstract

Background: Gamma-aminobutyric acid (Gaba) is a non-protein amino acid that plays important role in inhibition of neurotransmission. Gaba has been associated with various health benefit effects in human due to its biological activities. In this study, the optimal fermentation conditions for Gaba production from soybean solution fermented by Kimchi bacteria and its biological activities were investigated.
Material and Methods: Kimchi from market was diluted in 10 mL of 0.85% NaCl solution for bacterial collection. Gaba contents were determined by using colorimetry via mixing sample with phenol 6% and sodium hypochlorite 9%. Anti-oxidant assay was conducted via DPPH and ABTS scavenging ability. The ACE inhibitory assay was performed via measuring absorbance of hippuric acid formation and Dockingserve.
Results: The results showed that high Gaba content (13169 µg/10g soybean seeds) was found in the fermented product at pH value of 6.0 under temperature of 30 0C for 12h. Moreover, the anti-oxidant activity of Gaba-enriched soybean solution (1317 µg/mL) was observed due to scavenging 67% DPPH and 55% ABTS+ radicals. Notably, the Gaba-enriched soybean solution was showed to inhibit 43% ACE activity.
Conclusion: Accordingly, Gaba-enriched soybean solution was indicated as potential anti-oxidant and ACE inhibitory agent. It suggested that Gaba-enriched soybean solution was able to apply as health benefit foods with potential anti-oxidant and anti-hypertensive activities.

Keywords: ACE, DPPH, fermentation, Gaba, Kimchi

Citation: Sang VT, Uyen LP, Hung ND. The increased gamma-aminobutyric acid content by optimizing fermentation conditions of bacteria from kimchi and investigation of its biological activities. Eurasia J Biosci. 2018;12(2):369-76.

Determination of dominant serovars of Listeria monocytogenes strains isolated from spontaneous human abortion in Tehran/IRAN
Maryam Rezaei, Nadia Kazemi Pour, Jalil Vandyousefi, Farokh Rokhbakhsh Zamin, Gholamreza Irajian

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Abstract

Background: To assess the extent of Listeria monocytogenes dominant serotypes in the causing the human spontaneous abortion was used for culture, biochemical, API (Application Programming Interface) and PCR (Polymerase Chain Reaction) analysis for detecting the presence of virulence serotypes such as 1/2a and 4b in Tehran.
Methods: A total of 258 samples comprising placental bits, vaginal swabs and blood were collected from 123 patients with spontaneous abortion. Listeria monocytogenes was identified and confirmed by culture, biochemical, serological tests, API system, CAMP (Christie, Atkins, Munch and Petersen), hemolysis on sheep blood agar. PI-PLC (Phosphatidyl Inositol specific Phospholipase C) assay, followed by Multiplex PCR to detection of serotypes 1/2a and 4b.
Results: Out of 258 samples, 28 isolates of Listeria monocytogenes were identified   by different methods. All of the isolates confirmed by PCR. Our search, showed that from 123 patients, 28 of isolated (46.6%)  Listeria monocytogenes strains, 14(50%) belonged to serovar 1/2a, 10(35%) to serovar 4b and 4(14.2%) to other serovars respectively.
Result of the PCR method agreed with those the recovery of pathogenic dominant serotypes(1/2a,4b) isolated from cases of spontaneous abortion, indicates the significance of Listeria infection in pregnant women.
Conclusions: Based on our study, serovars 1/2a and 4b played a key role in the human spontaneous abortion. Data analysis also showed that these serovars (1/2a, 4b) are dominant serovars as a causative agent of the spontaneous abortion in pregnant women.

Keywords: dominant serovars, Listeria monocytogenes, spontaneous abortion

Citation: Rezaei M, Pour NK, Vandyousefi J, Zamin FR, Irajian G. Determination of dominant serovars of Listeria monocytogenes strains isolated from spontaneous human abortion in Tehran/IRAN. Eurasia J Biosci. 2018;12(2):377-83.

Identification of sbGnRH-GTHs system and estrogen receptor α (ER α) immunoreactivities in the mature testicular tissue in Rastrelliger brachysoma (Bleeker, 1851)
Sinlapachai Senarat, Jes Kettratad, Wannee Jiraungkoorskul, F. Gerald Plumley, Kanda Tongmit, Pisit Poolprasert, Niwat Kangwanrangsan, Masafumi Amano, Akio Shimizu, Piyakorn Boonyoung

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Abstract

Background: Comprehensive information of reproductive physiology in the testis of Rastrilliger brachysoma is not well known. In this study, the distributions of sbGnRH-GTHs system and estrogen receptor-α (ERα)–immunoreactivities (–ir) in the mature testicular tissue of male Rastrelliger brachysoma were described.
Material and Methods: Sexually mature Rastrelliger brachysoma, 17-18 cm with average 17.4 cm in standard length (n = 10 individual fish) were used and investigated using immunohistochemistry and immunofluorescence.
Results: Immunolocalization confirmed that the intense staining of sbGnRH, GTHs and ERα, in the mature testicular tissues of R. brachysoma were co-located and prominently observed in the Leydig cells and fibroblasts in the interstial compartment. Strong immunostaining of Sertoli cell was also observed, whereas intermediate/moderate immunoreactivity of the spermatogonia in the stromal compartment was seen.
Conclusion: This data is reported for the first time and indicated that the testicular tissue and spermatogenesis of the R. brachysoma may locally produce sbGnRH, GTHs and ERα, which all are probably concerned with reproductive activity.

Keywords: estrogen receptor, fish, gonadotropin-releasing hormone, short mackerel, spermatogonia

Citation: Senarat S, Kettratad J, Jiraungkoorskul W, Plumley FG, Tongmit K, Poolprasert P, et al. Identification of sbGnRH-GTHs system and estrogen receptor α (ER α) immunoreactivities in the mature testicular tissue in Rastrelliger brachysoma (Bleeker, 1851). Eurasia J Biosci. 2018;12(2):385-92.

Effect of mushroom cooked in olive oil on some physiological and biochemical parameters of human
Wijdan I.A. Abd-alwahab, Fahad K.Y. Al-dulaimi, Abeer Talib Abdulqader

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Abstract

The study included an experiment was aimed to know and investigate some of physiological and biochemical effects of mushroom (Agaricus bisporus) which cooked with olive oil on volunteer persons fed for 30 days. The results showed that feeding on mushroom (Agaricus bisporus) cooked with olive oil caused a significant decrease (P<0.05) in concentrations of glucose, cholesterol, TG, LDL-C, body weights, and significant increase (P<0.05) in HDL-C concentration, W.B.Cs and R.B.Cs count in compared with control group. While, the feeding of mushroom that cooked with olive oil resulted no significant variations in levels of urea, uric acid, Hb and PCV in comparison with the control group. The present results conclude that use mushroom (Agaricus bisporus) with olive oil are reducing the harmful lipids, glucose and enhancing the blood cells, accordingly, it’s maybe have a beneficial effects on the liver and the health of human body.

Keywords: mushroom, Agaricus bisporus, olive oil, lipid profile, glucose, blood cells

Citation: Abd-alwahab WI, Al-dulaimi FK, Abdulqader AT. Effect of mushroom cooked in olive oil on some physiological and biochemical parameters of human. Eurasia J Biosci. 2018;12(2):393-7.

Effect of priming and priming duration on some germination characteristics of Neptun cultivar Canola
Nasrollah Atashi Shirazi, Foroud Bazrafshan, Omid Alizadeh, Kourosh Ordookhani, Alireza Safahani Langroodi

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Abstract

This study performed to evaluation of priming and priming duration effects on some germination characteristics of Neptun cultivar Canola as factorial randomized complete block design with three replications at the Besat Research Center. The first factor treatments included salicylic acid in 3 levels (750, 1500 and 2250 M), ascorbic acid in 3 levels (200, 400 and 600 ppm), polyethylene glycol on 2 levels (-2 and -4 bar), potassium chloride 2 level (-5 and -10 bar) Brassinosteroid on 2 levels (20 and 40 micrograms per liter), gibberellic acid on 2 levels (100 and 200 mg), nano zinc chelate and nitrogen each at two levels (1 and 2 mg) and priming water, second factor included priming duration at 3 levels (3, 6 and 12 h). Totally, according to result it was founded that priming had positive effect on germination characteristics also it was determined that gibberellic acid, salicylic acid and Ascorbic acid had highest effects incompared to other priming, with increasing of concentration and duration of priming, studied traits increased in responses to treatments.

Keywords: Canola, Priming, Priming duration

Citation: Shirazi NA, Bazrafshan F, Alizadeh O, Ordookhani K, Langroodi AS. Effect of priming and priming duration on some germination characteristics of Neptun cultivar Canola. Eurasia J Biosci. 2018;12(2):399-403.

Problems of rational land use in agriculture
Tlektes Espolov, Aidos Espolov, Zhenisbek Suleimenov, Ibragim Seуtasanov, Garcin Tazhigulova, Ruslan Kultemirov

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Abstract

This article examines the major problems of land use in Kazakhstan existing in the climate of stepwise elaboration of the land law ensuring the rational use of land. The focus was laid on the events of strengthening the government control over the formation of land relations and laying down more effective administrative regulations on land use in agriculture, based on local differences and living conditions in the region. Legal entities and individuals owning land plots do not show sufficient interest in the effective use of land and in its preservation. The major reason why the land cover of land plots is disturbed is that the approach to assessing the state of agricultural land is one-size-fits-all and does not take into account the landscape characteristics of the land. The cure here is to follow the major provisions of the current land taxation system, which establish the cadastral (assessed) cost of those plots, as well as to bridge the legal gaps by introducing tax rates tied to local differences in agricultural activities. This will create equal grounds for all forms of economic entities in agriculture to develop, ensuring their interest in the rational use of lands they own.

Keywords: land code, land law, a cadastre, land management, land use, legal status

Citation: Espolov T, Espolov A, Suleimenov Z, Seуtasanov I, Tazhigulova G, Kultemirov R. Problems of rational land use in agriculture. Eurasia J Biosci. 2018;12(2):405-11.

Comparison of synthetic and natural Taxol extracted from Taxus plant (Taxus baccata) on growth of ovarian cancer cells under in vitro condition
Bibizahra Modarresi-Darreh, Kazem Kamali, Seyed Mehdi Kalantar, Hamid Dehghanizadeh, Behrouz Aflatoonian

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Abstract

At the current century, one of main challenges in human health which is costly is dealing with cancer disorders. Different approaches are applied to treat different types of cancers such as surgery, radiotherapy and chemotherapy. Recently, new technologies for drug targeting and using biosensors are developing fast to help as new solutions for cancer diseases. In this study, the anticancer potential of natural and synthetic Taxol/paclitaxel are evaluated using cell culture technique on ovarian cancer cell line (Ovcar3). Natural Taxol was extracted from Taxus baccata by two methods: classic or soaking and soxulate method. MTT assay was used for evaluation of anticancer activity of acquired extract. For this, cells were incubated with different concentration of extract. All compounds were tested in four independent experiments. The anticancer activity was recorded in three times: 24, 48, and 72 hours. According to results, the best condition for extraction of Taxol was soxulate method with ethanol as solvent. The results showed that the increase of Taxol concentration lead to higher toxicity on cancerous cells in examined three times. The comparison of activity of natural and chemical Taxol showed that there is no significant difference between two compounds at 24 and 72 hour. So, the similar observed activity for natural and chemical Taxol in this study is due to similar structures. Our study showed that the natural Taxol without any modification can use as anticancer agent

Keywords: anticancer activity, natural, synthetic, Taxol/paclitaxel, MTT, Soxhlet

Citation: Modarresi-Darreh B, Kamali K, Kalantar SM, Dehghanizadeh H, Aflatoonian B. Comparison of synthetic and natural Taxol extracted from Taxus plant (Taxus baccata) on growth of ovarian cancer cells under in vitro condition. Eurasia J Biosci. 2018;12(2):413-8.

Effect of hot aqueous extract of Rheum ribes roots on some hormonal and biochemical parameters in induced Polycystic Ovary Syndrome in local female rabbits
Aseel Mokdad Hatam AbdulWahed, Salih M. Rahem Al-obaidi, Abdul-monaim H. M. Al-samarrai

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Abstract

The study aimed to evaluate the effect of aqueous extract of R. ribes roots and metformin in treatment of induced PCOS. 40 adult female rabbits (1000-1600g) use in this study, 30 rabbits injected with TP (100mg/Kg) for 4 days consecutive and left 3 days for developing the syndrome. The female rabbits divided to four groups: C+ve group, C-ve group, G1 (R. ribes extract 300mg/Kg) and G2 (metformin group 20mg/Kg). Treatment period was 30 days. The hormonal and biochemical assays include: AMH, Testosterone, Insulin, HOMA-IR and Glucose levels. The results showed significant increase at P≥0.05 in levels of AMH, Testosterone, Insulin, HOMA-IR and Glucose in C+ve group compared to C-ve group while the treated groups (G1 and G2) showed significant decrease in a parameters compared with C+ve except Insulin levels in G2 which show non-significant difference compared to C-ve, C+ve and G1.

Keywords: PCOS, AMH, HOMA-IR, R. ribes

Citation: AbdulWahed AMH, Al-obaidi SMR, Al-samarrai AHM. Effect of hot aqueous extract of Rheum ribes roots on some hormonal and biochemical parameters in induced Polycystic Ovary Syndrome in local female rabbits. Eurasia J Biosci. 2018;12(2):419-23.

Physiological analysis of vitamin D deficiency in food and its impact on osteoporosis and heredity
Karem Kdaer Karem, Ali Mousa Al Mousawi, Saba Ibrahim Salih

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Abstract

The aim of this study is to find out the difference between the osteoporosis, heredity and controls in Serum 25-hydroxyvitamin D and serum calcium. And to determine the biochemical changes among patients with osteoporosis, heredity and to assess any association different demographic variables. A convenient sample of 50 patients diagnosed to have osteoporosis was compared with thirty controls known not to have osteoporosis. Serum 25-hydroxyvitamin D and calcium level were compared between the two groups. Frequency distribution tables with percentages and means and standard deviations were compared using the Statistical Package for Social Sciences version 21 (SPSS-21) at a significance level of <0.05. The age and gender distribution was similar in both groups. The mean age was 42.54 ± 16.19 and 41.67 ± 18.14 in the osteoporosis patients and controls, respectively. Highly significant difference was found in the mean vitamin D level between the two groups while no such difference was discovered in calcium level. Age was weakly, but significantly correlated with calcium level and there was a significant correlation between vitamin D and calcium level. Previous similar studies in surrounding counties and developed countries showed similar finding which needs to be translated to actions in the form of screening or general supplementation for elder people regardless of symptoms. Conclusions and recommendations: Patients with osteoporosis need to be treated with vitamin D therapy to alleviate their symptoms.

Keywords: osteoporosis, serum calcium, vitamin D deficiency

Citation: Karem KK, Al Mousawi AM, Salih SI. Physiological analysis of vitamin D deficiency in food and its impact on osteoporosis and heredity. Eurasia J Biosci. 2018;12(2):425-30.

Biologically active substance application efficiency for meat rabbit breeding
L. V. Alekseeva, A. A. Lukyanov, O. V. Bogdanova

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Abstract

Researchers of the present study set out the main results of a comprehensive analysis concerning the current state of Russian rabbit meat market. They determined technological and economic advantages of rabbit breeding in comparison with other types of farm animals. They confirmed experimentally the increase of breeding efficiency with the introduction of vitamin-mineral complex preparations into the diet of meat breed rabbits in the form of “Ushastik” premix and E-selenium.

Keywords: animal husbandry, rabbit breeding, meat market, biologically active substances, efficiency

Citation: Alekseeva LV, Lukyanov AA, Bogdanova OV. Biologically active substance application efficiency for meat rabbit breeding. Eurasia J Biosci. 2018;12(2):431-5.

Environmental Effects of Enhancement of Mechanical and Hydrophobic Properties of Polyester Fabrics Using Silica/Kaolinite/Silver Nanocomposite: A Facile Technique for Synthesis and RSM Optimization
Ehsan Zarrinabadi, Ramin Abghari, Ali Nazari, Mohammad Mirjalili

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Abstract

Polyester is cheap and versatile and for that reason it has become ubiquitous in fashion, but the environmental impacts of polyester are also significant. In this research environmental effects of an alkaline-activated polyester (AAP) was impregnated with three-component effective nano composite silica/kaolinite/silver through impregnation conventional process to improve mechanical properties using statistical approach of response surface methodology (RSM). Environmental impact can take various forms, some familiar and others not generally recognized. This process was conducted by photo reduction through changing the proportions of silica, kaolinite and silver nanoparticles and optimizing them with a design of experiments (DOE). Moreover, the influence of alkaline activation process on the polyester fabric properties was evaluated. The investigated mechanical characteristics included the strength, friction, abrasion and hydrophobicity of AAP modified with the silica/kaolinite/silver nanocomposite. Such identification and analysis techniques as scanning electron microscopy (SEM), energy dispersive x-ray (EDX) and Fourier transform infrared spectroscopy (FTIR) were employed to compare the features of AAP modified with the silica/kaolinite/silver nanocomposite to those of a control fabric. The results indicated that the AAP modified with the synthesized nanocomposite was significantly enhanced in terms of strength, abrasion, and hydrophobic properties. The production of polyester uses harmful chemicals, including carcinogens, and if emitted to water and air untreated, can cause significant environmental damage.

Keywords: environmental effects, alkaline-activated polyester, hydrophobic properties, mechanical properties, design of experiments

Citation: Zarrinabadi E, Abghari R, Nazari A, Mirjalili M. Environmental Effects of Enhancement of Mechanical and Hydrophobic Properties of Polyester Fabrics Using Silica/Kaolinite/Silver Nanocomposite: A Facile Technique for Synthesis and RSM Optimization. Eurasia J Biosci. 2018;12(2):437-50.

Agro-ecological analysis of optimization of extruding parameters of feed additive based on rice processing products
Saule T. Zhiyenbayeva, Shynar I. Kenenbay, Meruert P. Baiysbaeva, Bagila Sh. Zhetpisbaeva, Laila S. Syzdykova

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Abstract

Agroecology is the study of ecological processes applied to agricultural production systems. Bringing ecological principles to bear in agroecosystems can suggest novel management approaches that would not otherwise be considered. The results of agro-ecological analysis of optimization of extruding parameters of feed additive based on rice processing products with the use of methods of multiple-factor experiments planning, statistical processing of test data and search optimization were given in this article. The optimum of criterion functions was determined in Excel 2003 by the method of search optimization with the use of “Search of Decision” computer program. Trial tests of production of extruded feed additive based on rice and compound feeds processing containing rice for farm animals have confirmed the results of researches received in laboratory and experimental and bench conditions were carried out in industrial conditions.

Keywords: agro-ecological analysis, optimization, extruding, feed additive, rice screenings, rice grain waste, rice pod, methods of multiple-factor experiments planning, statistical data processing

Citation: Zhiyenbayeva ST, Kenenbay SI, Baiysbaeva MP, Zhetpisbaeva BS, Syzdykova LS. Agro-ecological analysis of optimization of extruding parameters of feed additive based on rice processing products. Eurasia J Biosci. 2018;12(2):451-6.

Biochemistry, synthesis and identification of (four, five, six and seven) membered heterocyclic derivatives from 4-nitro-5-methylimidazole
Rafid Kais Kmal, Shaimaa Adnan Behget

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Abstract

This work included biochemistry and synthesis new azo compound (1-(4-((5-methyl-4-nitro-4, 5-dihydro-1H-imidazol-2-yl) diazenyl) phenyl) ethan-1-one) by reaction 4-nitro-5-methylimidazole with 4-aminoacetophenone in acid medium, then react with 4-aminoantipyrine to get Schiff base derivative. React with (chloroacetylchloride, thioglycolic acid, glycine, alanine, sodium azide, and phthalic anhydride, maleic anhydride) to give (β-lactam, thiazolidine, imidazolidine, tetrazole, oxazepine) derivatives. The new synthesized compounds have been identified by their melting points, H-NMR, C-NMR and FT-IR spectra.

Keywords: Schiff bases, azo-schiff base, β-lactam

Citation: Kmal RK, Behget SA. Biochemistry, synthesis and identification of (four, five, six and seven) membered heterocyclic derivatives from 4-nitro-5-methylimidazole. Eurasia J Biosci. 2018;12(2):457-65.

Environmental evaluation of auxin priming and plant growth promoting rhizobacteria on yield and yield components of wheat under drought stress
Najmeh Bagheri, Omid Alizadeh, Shahram Sharafzadeh, Farshid Aref

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Abstract

This study was performed to the environmental evaluation of auxin and plant growth promoting rhizobacteria priming on yield and yield components of wheat under drought stress. Experiment was conducted as a Split plot in RCBD design with 3 replications. Main factor included drought stress (irrigation on base of 25, 50 and 75%FC) and sub plots were inoculation at 4 levels (control, azotobacter, pseudomonas and a combination of two bacteria), also sub plots were auxin priming at 4 levels (0, 1, 2 and 4 mg.L-1). According to results, application of drought stress led to the reduction grain yield, so, the 50% and 75% water discharge treatments led to 9% and 37% the reduction of grain yield as compared to control, respectively. In relation to bacteria treatments, highest grain yield (4.35 t/ha) obtained by azotobacter inoculation. In relation to auxin application, the 4 mg.L-1 treatment led to 6%the increase of grain yield as compared to control. Highest means of grain yield (3.25 t/ha) under 75% discharge water observed by azotobacter inoculation. We concluded that priming with auxin and rhizobacteria, plays an important role in the induction of tolerance to drought and overcome limitations created by the drought stress on wheat.

Keywords: environmental evaluation, bacteria, plant regulator, priming, yield, wheat

Citation: Bagheri N, Alizadeh O, Sharafzadeh S, Aref F. Environmental evaluation of auxin priming and plant growth promoting rhizobacteria on yield and yield components of wheat under drought stress. Eurasia J Biosci. 2018;12(2):467-2.

A biological analysis of endocrine-disturbing chemicals in camel meat sector in Kazakhstan
Aruzhan Shoman, Assiya Serikbayeva, Laura Mamayeva, Bernard Faye, Tamara Tultabayeva

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Abstract

Endocrine disrupting chemicals (EDCs) consist of a diverse group of industrial chemicals and pharmacological agents. The use of instrumental analyses as the first screening tool might not be cost-effective to identify the existence of enormous numbers of chemical contaminants in environments. Also, knowledge of the concentration of individual residues is difficult to use to evaluate biological impacts of contaminants to wildlife and humans. The primary objective of present paper is a biological analysis of camel meat status in Kazakhstan. After a post-independence decline linked to the restructuration of collective structures in agriculture and food sector, the camel sector increased regularly. The camel population increased annually by 0.5% on average since the independence, while camel meat production increased by 1.2%. The slaughtering rate appeared still high, but stable for 10 years. Camel meat represented 1% only of the total red meat consumed in the country but this proportion is increasing. Despite this growing interest for camel meat, the sector is not organized in Kazakhstan. Despite recent initiatives in big towns the breeding is still traditional, and the consumption is essentially rural. Moreover, there are very few processing and no standard regarding this meat. The perspectives of development require however, the establishment of formal rules.

Keywords: biological review, camel, meat sector, processed meat, meat product

Citation: Shoman A, Serikbayeva A, Mamayeva L, Faye B, Tultabayeva T. A biological analysis of endocrine-disturbing chemicals in camel meat sector in Kazakhstan. Eurasia J Biosci. 2018;12(2):473-9.

Friction coefficient and biophysical properties for UPE-PMMA blend reinforced by nano-zinc oxide
Mohammed O. Kadhim, Fadhil K. Farhan, Dhilal A. Sabar, Osama N. Abood

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Abstract

In this study, nanocomposite samples were prepared from (80 vol. % unsaturated polyester (UPE) with 20 vol. % Poly methyl methacrylate (PMMA)] blend matrix and reinforced by ZnO nanoparticles with average particle size of 72nm and with different percentage (0.0.2, 0.4, 0.6, 0.8 and 1vol. %) by using Ultrasonic dispersion technique to obtain a homogeneous mixture free of aggregates. The structure and particles size of nanoparticles were investigated by atomic force microscope (AFM) and energy dispersive x-ray Spectroscopy (EDX). Wear rate, coefficient of friction and heat of friction have been measured by (Pin-on-disc) technique. The surface morphology of nanocomposite samples was studied by scanning electron microscopic (SEM) to aid interpretation results of sliding wear, and distribution nanoparticle in polymer (base-matrix). Experimental results of Tribological and biophysical properties (Dry sliding wear rate) show that values decreases progressively with increasing load and percentage of fillers.

Keywords: wear, friction, nano-ZnO, nanocomposite

Citation: Kadhim MO, Farhan FK, Sabar DA, Abood ON. Friction coefficient and biophysical properties for UPE-PMMA blend reinforced by nano-zinc oxide. Eurasia J Biosci. 2018;12(2):481-6.

Abiotic stress responses in cereals and pulses crop and their agronomic practices to enhance tolerance
Akash Hidangmayum, Ankita Singh, Vivek Kumar, Padmanabh Dwivedi

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Abstract

Abiotic stresses are considered as major constraints to crop production due to the rapid changes in the global climate and threat to the food security along with environmental sustainability. Drought, heat, and salinity are important abiotic stresses causing a huge impact on agricultural productivity. Crop responses to this stresses are an important aspect to consider for management options. This review generalized the crop responses under abiotic stresses in the certain aspect related to morphological, physiological and biochemical responses on some major cereals and pulses crop. Better crop management practices such as conservation tillage, zero tillage and increasing water use efficiency along with the application of micronutrient and phytohormones to combat those stresses are comprehensively discussed in this review.

Keywords: abiotic stress, conservation tillage, micronutrient, phytohormones, water-use-efficiency, zero tillage

Citation: Hidangmayum A, Singh A, Kumar V, Dwivedi P. Abiotic stress responses in cereals and pulses crop and their agronomic practices to enhance tolerance. Eurasia J Biosci. 2018;12(2):487-93.

Spectrophotometric method to quantitative determination of propranolol in pharmaceutical form and human urine: a biochemical process
Ula Musaab, Khalaf F Alsamarrai, Abdulsalam Tewfeeq Salih Alsamarrai

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Abstract

This study included the development of a simple, sensitive, accurate, fast and economical spectrophotometric method to quantitative determination of propranolol in its pure, pharmaceutical form and human urine, the method is based on the reaction of propranolol PRO with Ninhydrin NIN to form a complex of high absorption at 292 nm. The method was liner over the concentration (20 - 80μg/mL), the value of (r2) was 0.999. The value of relative standard deviation (RSD %) ranged between (1.869-0.209%), the value of recovery percentage (Rec %) between (103.131-96.366%), the detection limit and quantification limit (LOD) and LOQ were (1.0615 μg / mL) and (3.5384 μg / mL) respectively. The absorptivity was (5.232x103 L / mol.cm), Sandell’s index (0.0495 μg / cm2) this method successfully applied to the determination of propranolol in pharmaceuticals forms and human urine.

Keywords: propranolol, ninhydrin, spectrophotometry, human urine

Citation: Musaab U, Alsamarrai KF, Alsamarrai ATS. Spectrophotometric method to quantitative determination of propranolol in pharmaceutical form and human urine: a biochemical process. Eurasia J Biosci. 2018;12(2):495-8.

The biological growth and development of lambs degeres fat-tailed sheep breed
Sh. R. Adylkanova, T. S. Sadykulov, G. L. Kim, A. M. Koishibayev, S. Yu. Dolgopolova

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Abstract

This article presents the results of studying the biological growth and development of young stock of different types of intra-breeding degeres fat-tailed breed of sheep. The experimental part of this work was carried out in the new environmental conditions of breeding for this breed of sheep in the foothill zone of the South-East of Kazakhstan, Almaty region.

Keywords: genotype, alive mass, postnatal period, ontogenesis, exterior measurements, body indices, homeostasis, intra-breed type, coefficient of variability, sheep

Citation: Adylkanova SR, Sadykulov TS, Kim GL, Koishibayev AM, Dolgopolova SY. The biological growth and development of lambs degeres fat-tailed sheep breed. Eurasia J Biosci. 2018;12(2):499-502.

The prophylactic –protective effect of camel milk on ethanol induced hepato-toxicity in newborn rats
Mohammed Talat Abbas, Ali J. Ali, Alaa Abd-Alhasan Hamdan

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Abstract

Objective: To assessment the effect of camel milk on ethanol induced hepatotoxicity in newborn rats.
Material and Methods: The study was conducted on 5 groups of 6 rats each, including healthy group (0.5ml normal saline (NS),gavage),ethanol group (3g/kg/day ethanol by gavage),camel milk group (1mL/kg/day/orally camel milk),ethanol(3g/kg/day/orally) plus camel milk (1mL/kg/day/orally )group, 1mL/kg/day/orally camel milk for fifteen days before gestation (as a prophylactic ) and then given ethanol(3g/kg/day/orally) group. The duration of treatment lasted from conception to birth.
Results: According to our findings in this study, the treatment of rats with ethanol during pregnancy leads to a significant increase in the level of MDA and to a significant decrease in the activity of SOD, CAT and GSH-PX enzymes in liver tissues of newborn rats, and histological changes in the liver tissue. In addition to a significant increase in the activity of serum liver enzymes compared to the control group.
Conclusion: We conclude from this study that camel milk has a protective and prophylactic effects against ethanol induced hepatotoxicity in newborn rats.

Keywords: ethanol, camel milk, oxidative stress, newborn rats

Citation: Abbas MT, Ali AJ, Hamdan AA. The prophylactic –protective effect of camel milk on ethanol induced hepato-toxicity in newborn rats. Eurasia J Biosci. 2018;12(2):503-9.

Unregistered marriage as modern practice of students’ youth family relations
Elena I. Cherdymova, Elizaveta V. Rozanova, Yulia V. Dementieva, Nina I. Kryukova, Olga V. Popova, Ruslan M. Mirzalimov, Yuriy M. Alpatov

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Abstract

The problem of creating a family, namely, the new configuration of marriage and intimate relations - unregistered marriage is becoming increasingly important in modern society. Unregistered marriage is the most common and socially approved model for organizing family life, taking into account the large similarity with a registered marriage. Young men and girls are currently not in a hurry to have families and children, but create so-called unregistered marriages. In this regard, the purpose of this study is to allocate the leading motives for creating just such a form of marriage among student youth, rather than traditionally accepted in Russia. The leading method of researching this problem is an in-depth interview, which allows considering this problem and identifying the particular practices and problems of living in an unregistered marriage of students. The article presents the results of a study of the students’ attitude towards unregistered marriage. It was revealed that the basis of unregistered marriage in most cases is the psychological unwillingness of partners to take full responsibility for the family. Most couples surveyed said that they plan to have children only in a formal marriage, since most of the girls surveyed would not feel legally protected, giving birth to a child in an unregistered marriage. The data obtained in the work can be used in family counseling, in family centers by practicing psychologists and teachers, as well as for further theoretical development of this issue, since the problem of various types of marriage and family relations is quite relevant today. This is a new area, where there are still many unresolved issues that require further study.

Keywords: family, extramarital ties, marriage and family relations, students

Citation: Cherdymova EI, Rozanova EV, Dementieva YV, Kryukova NI, Popova OV, Mirzalimov RM, et al. Unregistered marriage as modern practice of students’ youth family relations. Eurasia J Biosci. 2018;12(2):511-7.

A new method for determining the type of antimicrobial effect of compounds with antimicrobial activity
Irina S. Stepanenko

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Abstract

Objective: The study of the antimicrobial activity of new compounds includes both the traditional study of the minimum inhibitory concentration (MIC) of a substance and other indicators, one of which is the determination of its type of action (bactericidal or bacteriostatic). The goal of our study it as to develop a new method for determining the antimicrobial effect (bacteriostatic or bactericidal) based on the calculation of the optical density coefficient of the culture medium (СD) during the cultivation of microorganisms with antimicrobial compounds in a liquid nutrient medium (Muller-Hinton broth), within a rather short time interval of 24 hours.
Material and Methods: Methods used for determining the type of antimicrobial action require long observation for 5 days or more and high consumption of the studied compounds and of nutrient media.
Results: The novelty of the method is in developing empirically a formula for calculating and determining the value of the optical density coefficient of the culture medium that correlate with a bacteriostatic type of action and a bactericidal type of action of the studied compounds, within a rather short time interval of 24 hours. As a result of using the new method, the type of action of the new group of compounds was investigated. Derivatives of substituted aminoindoles have a bacteriostatic type of antimicrobial action against the studied microorganisms.
Conclusion: A new method of research allows to rapidly and correctly determining the type of antimicrobial action of new compounds. The CD coefficient in the presence of the test compounds is more than 1. This indicates that the test compounds synthesized on the basis of substituted 4-, 5-, 6, 7-aminoindoles have a bacteriostatic type of antimicrobial action against the studied microorganisms.

Keywords: antimicrobial compounds, type of antimicrobial action, aminoindoles, pyrroloquinolines, optical density of the culture medium

Citation: Stepanenko IS. A new method for determining the type of antimicrobial effect of compounds with antimicrobial activity. Eurasia J Biosci. 2018;12(2):519-25.

Socio-environmental aspects of the waste recycling organization
Vladimir V. Filatov, Natalia A. Zaitseva, Anna A. Larionova, Elena E. Rodina, Vasily I. Eroshenko, Natalia O. Vikhrova, Oksana V. Takhumova

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Abstract

The problems of organizing and managing the recycling of industrial waste have recently been more and more often raised not only as a part of environmental research, but also when discussing the economic development strategies of countries and individual regions. The relevance and timeliness of studying the socio-environmental aspects of organizing the recycling of industrial waste is due to the fact that the implementation of the concept of sustainable development of territories is impossible without finding effective solutions to solve not only economic and social, but also environmental problems. The purpose of the preparation of this article was the synthesis of international experience in the organization of recycling of production wastes. As the main methods of research, the method of descriptive statistics and methods of multivariate statistical analysis (cluster, correlation and regression analysis), content analysis, factor analysis, data systematization, method of expert assessments, method of coordination of expert assessments, and other scientific approaches that allow a comprehensive review of the studied problem were used. The study summarized the best practices in the organization and management of recycling of industrial wastes in developed and developing countries, on the basis of which it was concluded that it is necessary to create an effective interaction between government and market mechanisms to solve this problem. Materials of the article can be used in the development of a social-ecological strategy for the development of a system for recycling regional production bypasses.

Keywords: recycling, ecology, production wastes, waste recycling, utilization

Citation: Filatov VV, Zaitseva NA, Larionova AA, Rodina EE, Eroshenko VI, Vikhrova NO, et al. Socio-environmental aspects of the waste recycling organization. Eurasia J Biosci. 2018;12(2):527-33.