DNA fingerprinting is an important tool in criminal investigations. Typing of Variable Number Tandem Repeat (VNTR) is a genotyping tool that provides a numeric and simple format of data based on the repetitive sequences number. The present study uses DNA fingerprinting by amplify certain specific VNTR regions present at a particular locus of different chromosomes. The biological samples include available materials such as hair and blood present on a scene of crime, blood, buccal and along with fingernail. By amplifying he specific VNTR fragments of different DNA samples & comparing them on agarose gel electrophoresis, we obtained DNA profile of individuals, which in turn helps in criminal identification. It can also indicate whether the individuals are homozygous or heterozygous.

• Anchordoquy, H. C.;McGeary, C.;Liu, L.;Krauter, K. S. & Smolen, A. (2003). Genotyping of three candidate genes after whole-genome preamplification of DNA collected from buccal cells. Behavior genetics, 33(1), 73-78.
• Blackburn, G. M.;Gait, M. J.;Loakes, D.;Williams, D. M.;Egli, M.;Flavell, A.;Allen, S.;Fisher, J.;Haq, S. I. & Engels, J. W. (2006). Nucleic acids in chemistry and biology, Royal Society of Chemistry:
• Butler, J. M. (2007). Short tandem repeat typing technologies used in human identity testing. Biotechniques, 43(4), Sii-Sv.
• Butler, J. M. (2009). Fundamentals of forensic DNA typing, Academic press:
• Cunningham, E. & Meghen, C. (2001). Biological identification systems: genetic markers. Revue Scientifique et Technique-Office International des Epizooties, 20(2), 491-499.
• Hammond, H. A.;Redman, J. B. & Caskey, C. T. (1995). In utero paternity testing following alleged sexual assault: a comparison of DNA-based methods. JAMA, 273(22), 1774-1777.
• Hamzah, K. J. & Hasso, S. A. (2019). Molecular prevalence of Anaplasma phagocytophilum in sheep from Iraq. Open Veterinary Journal, 9(3), 238–245.
• Kim, J.;Jung, J. Y.;Kwon, S. Y.;Kang, P.;Park, H.;min Seong, K.;ue Kim, T.;Yoon, H. K. & Lim, S.-K. (2019). Development and forensic validation of human genomic DNA quantification kit. International journal of legal medicine, 1-13.
• Lee, H. Y.;Chung, U.;Park, M. J.;Yoo, J. E.;Han, G. R. & Shin, K. J. (2006). Differential distribution of human mitochondrial DNA in somatic tissues and hairs. Annals of human genetics, 70(1), 59-65.
• Musimba, N., Kimatu, J. N., Mweu, B., Mburu, M. W. K., & Nguluu, S. (2015). Germination Effects of Purposive Bruchid Screening of African Ebony (Dalbergia Melanoxylon) Seeds In the Arid and Semi-Arid Region of South Eastern Kenya. Current Research in Agricultural Sciences, 2(2), 60-66.
• Nübel, U. e.;Engelen, B.;Felske, A.;Snaidr, J.;Wieshuber, A.;Amann, R. I.;Ludwig, W. & Backhaus, H. (1996). Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. Journal of bacteriology, 178(19), 5636-5643.
• Parker, P. G.;Snow, A. A.;Schug, M. D.;Booton, G. C. & Fuerst, P. A. (1998). What molecules can tell us about populations: choosing andusing a molecular marker. Ecology, 79(2), 361-382.
• Pray, L. (2008). Discovery of DNA structure and function: Watson and Crick. Nature Education, 1(1), 100.
• Ramazanzadeh, R. & McNerney, R. (2007). Variable number of tandem repeats (VNTR) and its application in bacterial epidemiology. Pak J Biol Sci, 10(16), 2612-2621.
• Rogers, N. L.;Cole, S. A.;Lan, H. C.;Crossa, A. & Demerath, E. W. (2007). New saliva DNA collection method compared to buccal cell collection techniques for epidemiological studies. American Journal of Human Biology: The Official Journal of the Human Biology Association, 19(3), 319-326.
• Shewale, J. G. & Liu, R. H. (2013). Forensic DNA analysis: current practices and emerging technologies, CRC Press:
• Silva, R. H. A. d.;Sales-Peres, A.;Oliveira, R. N. d.;Oliveira, F. T. d. & Sales-Peres, S. H. d. C. (2007). Use of DNA technology in forensic dentistry. Journal of Applied Oral Science, 15(3), 156-161.
• Sreenan, J. J.;Pettay, J. D.;Tbakhi, A.;Totos, G.;Sandhaus, L. M.;Miller, M. L.;Bolwell, B. & Tubbs, R. R. (1997). The use of amplified variable number of tandem repeats (VNTR) in the detection of chimerism following bone marrow transplantation: a comparison with restriction fragment length polymorphism (RFLP) by Southern blotting. American journal of clinical pathology, 107(3), 292-298.
• Sumi, H.;Naito, E.;Dewa, K.;Fukuda, M.;Xu, H.-D. & Yamanouchi, H. (2005). Applicability of the parentally imprinted allele (PIA) typing of a VNTR upstream the H19 gene to forensic samples of different tissues. Legal Medicine, 7(3), 179-182.
• Supply, P.;Allix, C.;Lesjean, S.;Cardoso-Oelemann, M.;Rüsch-Gerdes, S.;Willery, E.;Savine, E.;de Haas, P.;van Deutekom, H. & Roring, S. (2006). Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis. Journal of clinical microbiology, 44(12), 4498-4510.
• Varma-Basil, M.;Kumar, S.;Arora, J.;Angrup, A.;Zozio, T.;Banavaliker, J. N.;Singh, U. B.;Rastogi, N. & Bose, M. (2011). Comparison of spoligotyping, mycobacterial interspersed repetitive units typing and IS6110-RFLP in a study of genotypic diversity of Mycobacterium tuberculosis in Delhi, North India. Memorias do Instituto Oswaldo Cruz, 106(5), 524-535.
• Vergnaud, G. & Pourcel, C. (2006). Multiple locus VNTR (variable number of tandem repeat) analysis Molecular identification, systematics, and population structure of prokaryotes (pp. 83-104): Springer.

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