Uncompensated response to the oxidation stress of the diabetes in both male and female patients with type II diabetes

Abstract

Background: This study evaluated the level of few antioxidant enzymes through the variation in these enzymatic biomarkers in type II diabetes.
Objectives: The present study aimed to report the variation of some enzymatic antioxidant parameters level as uncompensated response to the oxidation stress in type II diabetes patients.
Materials and Methods: 45 diabetic patients and 44 healthy subjects were recruited (aged 20- 71 years) in Kirkuk province northern Iraq. The levels of (HbA1c) and (FBS), (SOD), (TAS), (GP) and (GPx), were evaluated. The glucose was measured in the patient’s serum after 12 hours of fasting following a standard procedure in the kit from (Rondo. United Kingdom Laboratories Ltd), while HbA1c calculated using Stanbio kit (USA). The Antioxidant enzymes, SOD, TAS, GP and GPx were assessed in serum samples using colorimetric Randox kit (Randox, laboratories, Ltd). Body mass index (BMI) was calculated using spscific formula and classifying normal weight as (BMI 18.5- 24.9) Kg/M2, obesity as (BMI 30-39.9) Kg/M2 and morbid obesity as (BMI > 40) Kg/M2).The waist circumference was measured at the narrowest point of the torso width-wise, usually just above the belly button, which is ≤ 102 cm in male and≤88 cm in female.
Results: Plasma (HbA1c) concentrations and (FBS) are higher in patients than in controls (P = 0.04* and 0.001*, respectively). Surprisingly, the amount of (GPx) and (SOD) are lower in patients group comparing to control group (P < 0.002* and P < 0.05*, respectively). Concentrations of (TAS), in contrast, is significantly lower in patients than controls group (P = 0.002*), while no change is noticed in the level of (GR) in patients groups (P = 0.73). There are significant differences (P≤ 0.05) in a mean of age, BMI, waist circumference, (FBS), (GPx), (TAS) and (HbA1c) among female and male of two groups. There are no other statistically significant differences (P ≥ 0.05) between gender in health and patient groups in relation to the serum levels of (GR) and (SOD) enzymes. In addition, data showed there is no significant correlation (P ≥ 0.05) (HbA1c) and antioxidant enzymes concentration in type II diabetes patients.
Conclusions: Enzymatic biomarkers can be used to monitor the uncompensated response to the oxidation stress as shown by lower antioxidant level in type II diabetes patients in this study.

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