Uncompensated response to the oxidation stress of the diabetes in both male and female patients with type II diabetes


Background: This study evaluated the level of few antioxidant enzymes through the variation in these enzymatic biomarkers in type II diabetes.
Objectives: The present study aimed to report the variation of some enzymatic antioxidant parameters level as uncompensated response to the oxidation stress in type II diabetes patients.
Materials and Methods: 45 diabetic patients and 44 healthy subjects were recruited (aged 20- 71 years) in Kirkuk province northern Iraq. The levels of (HbA1c) and (FBS), (SOD), (TAS), (GP) and (GPx), were evaluated. The glucose was measured in the patient’s serum after 12 hours of fasting following a standard procedure in the kit from (Rondo. United Kingdom Laboratories Ltd), while HbA1c calculated using Stanbio kit (USA). The Antioxidant enzymes, SOD, TAS, GP and GPx were assessed in serum samples using colorimetric Randox kit (Randox, laboratories, Ltd). Body mass index (BMI) was calculated using spscific formula and classifying normal weight as (BMI 18.5- 24.9) Kg/M2, obesity as (BMI 30-39.9) Kg/M2 and morbid obesity as (BMI > 40) Kg/M2).The waist circumference was measured at the narrowest point of the torso width-wise, usually just above the belly button, which is ≤ 102 cm in male and≤88 cm in female.
Results: Plasma (HbA1c) concentrations and (FBS) are higher in patients than in controls (P = 0.04* and 0.001*, respectively). Surprisingly, the amount of (GPx) and (SOD) are lower in patients group comparing to control group (P < 0.002* and P < 0.05*, respectively). Concentrations of (TAS), in contrast, is significantly lower in patients than controls group (P = 0.002*), while no change is noticed in the level of (GR) in patients groups (P = 0.73). There are significant differences (P≤ 0.05) in a mean of age, BMI, waist circumference, (FBS), (GPx), (TAS) and (HbA1c) among female and male of two groups. There are no other statistically significant differences (P ≥ 0.05) between gender in health and patient groups in relation to the serum levels of (GR) and (SOD) enzymes. In addition, data showed there is no significant correlation (P ≥ 0.05) (HbA1c) and antioxidant enzymes concentration in type II diabetes patients.
Conclusions: Enzymatic biomarkers can be used to monitor the uncompensated response to the oxidation stress as shown by lower antioxidant level in type II diabetes patients in this study.


  • Arthur, J. R. (2001). The glutathione peroxidases. Cellular and Molecular Life Sciences CMLS, 57(13-14), 1825-1835.
  • Asmat, U., Abad, K., & Ismail, K. (2016). Diabetes mellitus and oxidative stress—A concise review. Saudi Pharmaceutical Journal, 24(5), 547-553.
  • Astrup, A. (2001). Healthy lifestyles in Europe: prevention of obesity and type II diabetes by diet and physical activity. Public health nutrition, 4(2b), 499-515.
  • Bandeira, S. D. M., Guedes, G. D. S., Fonseca, L. J. S. D., Pires, A. S., Gelain, D. P., Moreira, J. C. F.,... & Goulart, M. O. F. (2012). Characterization of blood oxidative stress in type 2 diabetes mellitus patients: increase in lipid peroxidation and SOD activity. Oxidative medicine and cellular longevity, 2012.
  • Decode-Decoda Study Group, & European Diabetes Epidemiology Group. (2003). Age, body mass index and type 2 diabetes—associations modified by ethnicity. Diabetologia, 46(8), 1063-1070.
  • Fei, B. B., Ling, L., Hua, C., & Ren, S. Y. (2014). Effects of soybean oligosaccharides on antioxidant enzyme activities and insulin resistance in pregnant women with gestational diabetes mellitus. Food chemistry, 158, 429-432.
  • Gawlik, K., Naskalski, J. W., Fedak, D., Pawlica-Gosiewska, D., Grudzień, U., Dumnicka, P.,... & Solnica, B. (2016). Markers of antioxidant defense in patients with type 2 diabetes. Oxidative medicine and cellular longevity, 2016.
  • Högestätt, E. D., Andersson, K. E., & Edvinsson, L. (1983). Mechanical properties of rat cerebral arteries as studied by a sensitive device for recording of mechanical activity in isolated small blood vessels. Acta Physiologica Scandinavica, 117(1), 49-61.
  • Johnson, F., & Giulivi, C. (2005). Superoxide dismutases and their impact upon human health. Molecular aspects of medicine, 26(4-5), 340-352.
  • Kumar, V., Khanna, A. K., Khan, M. M., Singh, R., Singh, S., Chander, R.,... & Singh, R. K. (2009). Hypoglycemic, lipid lowering and antioxidant activities in root extract of Anthocephalus indicus in alloxan induced diabetic rats. Indian Journal of Clinical Biochemistry, 24(1), 65-69.
  • Lee, H., Song, J., Jung, J. H., & Ko, H. W. (2015). Primary cilia in energy balance signaling and metabolic disorder. BMB reports, 48(12), 647.
  • Maritim, A. C., Sanders, A., & Watkins Iii, J. B. (2003). Diabetes, oxidative stress, and antioxidants: a review. Journal of biochemical and molecular toxicology, 17(1), 24-38.
  • Martinon, F. (2010). Signaling by ROS drives inflammasome activation. European journal of immunology, 40(3), 616-619.
  • Mohamed, A. K. (2015). Assess the relationship between insulin fasting levels and healthy obesity in adults in the province of Babylon, Iraq. Iraqi National Journal of Chemistry, 15(4), 318-328.
  • Mohammed, A. K., Mokif, T. A., & Hadwan, M. H. (2019). The effect of Morbid Obesity on the Liver Function Enzymes. Research Journal of Pharmacy and Technology, 12(5), 2241-2244.
  • Naudi, A., Jove, M., Ayala, V., Cassanye, A., Serrano, J., Gonzalo, H.,... & Pamplona, R. (2012). Cellular dysfunction in diabetes as maladaptive response to mitochondrial oxidative stress. Experimental diabetes research, 2012.
  • Rahbani-Nobar, M. E., Rahimi-Pour, A., Rahbani-Nobar, M., Adi-Beig, F., & Mirhashemi, S. M. (1999). Total antioxidant capacity, superoxide dismutase and glutathione peroxidase in diabetic patients. Medical Journal of Islamic Academy of Sciences, 12(4), 109-114.
  • Salari, P., Lashkaripour, G., & Ghafoori, M. (2015). Presentation of Empirical Equations for Estimating Internal Friction Angle of SP and SC Soils in Mashhad, Iran Using Standard Penetration and Direct Shear Tests and Comparison with Previous Equations. International Journal of Geography and Geology, 4(5), 89.
  • Saudek, C. D., Kalyani, R. R., & Derr, R. L. (2005). Assessment of Glycemia in Diabetes Mellitus: Hemoglobin A. JAPI, 53.
  • Shingu, M., Takahashi, S., Ito, M., Hamamatu, N., Suenaga, Y., Ichibangase, Y., & Nobunaga, M. (1994). Anti-inflammatory effects of recombinant human manganese superoxide dismutase on adjuvant arthritis in rats. Rheumatology international, 14(2), 77-81.
  • Son, S. M., Whalin, M. K., Harrison, D. G., Taylor, W. R., & Griendling, K. K. (2004). Oxidative stress and diabetic vascular complications. Current diabetes reports, 4(4), 247-252.
  • Tietz, N. W. (1999). Textbook of clinical chemistry.(3rdedn.) WB SaundersCompany.
  • Van der Does, F. E., De Neeling, J. N. D., Snoek, F. J., Kostense, P. J., Grootenhuis, P. A., Bouter, L. M., & Heine, R. J. (1996). Symptoms and well-being in relation to glycemic control in type II diabetes. Diabetes care, 19(3), 204-210.
  • Villareal, D. T., Apovian, C. M., Kushner, R. F., & Klein, S. (2005). Obesity in older adults: technical review and position statement of the American Society for Nutrition and NAASO, The Obesity Society. Obesity research, 13(11), 1849-1863.
  • Waggiallah, H., & Alzohairy, M. (2011). The effect of oxidative stress on human red cells glutathione peroxidase, glutathione reductase level, and prevalence of anemia among diabetics. North American journal of medical sciences, 3(7), 344.
  • Wilkin, T. J. (2009). The accelerator hypothesis: a review of the evidence for insulin resistance as the basis for type I as well as type II diabetes. International Journal of obesity, 33(7), 716-726.


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