Background: Chitosan is a material that is biocompatible, biodegradable, bioadhesive, and nontoxic when it comes to human cells. Chitosan has potential to be used as an antimicrobial and antioxidant material. Chitosan’s biocompatibility still depends on its origin, method of manufacture, and degree of deacetylation. To be used as an alternative scaffold material for bone augmentation that is safe to use in the field of dentistry, the authors are interested in conducting toxicity tests on BHK-21 fibroblast cell cultures.
Aim: To determine the toxicity of chitosan from shrimp shells (Litopenaeus Vannamei) on BHK-21 fibroblast cell cultures.
Method: This type of research is a laboratory experiment with a Post Test Only Control Group Design. Treatment was conducted by administration of chitosan from shrimp shells (Litopenaeus Vannamei) with a concentration of 100%, 75%, 50%, and 25% on the BHK 21 fibroblast cell cultures.
Results: The percentage of remaining fibroblast cells at concentrations of 100%, 75%, 50%, and 25% respectively were 51%, 53%, 54%, and 56%. Toxicity results were obtained by using the MTT assay technique after 24 hours. Optical density absorbance values describe the viability of living cells and the readings were conducted by using ELISA readers.
Conclusion: The results of the toxicity test of shrimp shells (Litopenaeus Vannamei) chitosan did not show toxic effects on BHK-21 fibroblast cells.

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