AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Hafsan, Agustina L, Natsir A, Ahmad A. The stability of Phytase activity from Burkholderia sp. strain HF.7. Eurasia J Biosci. 2020;14(1), 991-994.

APA 6th edition
In-text citation: (Hafsan et al., 2020)
Reference: Hafsan, Agustina, L., Natsir, A., & Ahmad, A. (2020). The stability of Phytase activity from Burkholderia sp. strain HF.7. Eurasian Journal of Biosciences, 14(1), 991-994.

Chicago
In-text citation: (Hafsan et al., 2020)
Reference: Hafsan, Laily Agustina, Asamuddin Natsir, and Ahyar Ahmad. "The stability of Phytase activity from Burkholderia sp. strain HF.7". Eurasian Journal of Biosciences 2020 14 no. 1 (2020): 991-994.

Harvard
In-text citation: (Hafsan et al., 2020)
Reference: Hafsan, Agustina, L., Natsir, A., and Ahmad, A. (2020). The stability of Phytase activity from Burkholderia sp. strain HF.7. Eurasian Journal of Biosciences, 14(1), pp. 991-994.

MLA
In-text citation: (Hafsan et al., 2020)
Reference: Hafsan et al. "The stability of Phytase activity from Burkholderia sp. strain HF.7". Eurasian Journal of Biosciences, vol. 14, no. 1, 2020, pp. 991-994.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Hafsan, Agustina L, Natsir A, Ahmad A. The stability of Phytase activity from Burkholderia sp. strain HF.7. Eurasia J Biosci. 2020;14(1):991-4.

Abstract

Objectives: Phytase is an enzyme that hydrolyzes phytic acid but is not produced in the monogastric gastrointestinal tract of poultry. This study aims to discover the stability of extracellular phytase Burkholderia sp. strain HF.7 under a range of temperature, pH, and protease activity conditions including those similar to the digestive tract of poultry. Material and Methods: Temperature and pH stability tests were carried out at various temperatures (20-65 °C) and pH (1-8) by incubating the phytase in a Na-acetate buffer. The stability of phytase protease was further tested by adding the following levels of proteases (pepsin and/or trypsin): P0: without protease, P1: pepsin (5000 units/mL), P2: trypsin (5000 units/mL) and P3: pepsin + trypsin (2500 units/mL for each). ANOVA was used to analyze the protease stability experiments (α=0.05). Finding: The results showed that B. sp. strain HF.7 phytase had an optimum activity at 37 °C and pH 4; similar to the condition of the poultry digestive tract in general. Conclusion: These findings suggest that B. sp. strain HF.7 has potential to be used in poultry feed to increase productivity.

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