The role of Moringa leaf extract to reduce the negative effect of some food additives on gene expression of CYP11A1 gene in male albino rats

Abstract

This study aimed to investigate the effect of Moringa oleifera leaf extract on the gene expression of the Cyp11A1 gene in male white rats treated with some food additives (Carmoisine, Sodium benzoate, Monosodium glutamate).The study included 40 male rats divided into eight groups and each group consist of 5 animals dosed for 60 days. The groups were divided as follows: Negative control group (C1) given drinking water only, Positive control group(C2) given Moringa leaf extract in dose(200mg/kg/B.W),The first treatment group (T1) given orally Carmoisine in the dose (250mg/kg/B.W),The second treatment group (T2) given orally monosodium glutamate in the dose (15mg/kg/B.W),The third group (T3) given orally Sodium benzoate in the dose (50mg/kg/B.W),The fourth group (T4) given orally Carmoisine in the dose(250mg/kg/B.W) then given Moringa leaf extract in the dose(200mg/kg/B.W) Concurrently, The fifth group (T5) given orally monosodium glutamate in the dose (15mg/kg/B.W) then given Moringa leaf extract in the dose(200mg/kg/B.W) Concurrently, and The sixth group (T6) given orally Sodium benzoate in the dose (50mg/kg/B.W) then given Moringa leaf extract in the dose(200mg/kg/B.W) Concurrently. The results showed a significant decrease (P <0.05) in the level of gene expression of the CYP11A1 gene in animal groups (T1, T2, T3) which were given food additives (Carmoisine, Monosodium Glutamate, Sodium Benzoate) compared with the negative and positive control groups. No significant differences between the three groups mentioned. As for the groups (T4, T5, T6), no significant differences appeared between the mentioned groups, but showed a significant increase (P <0.05) compared to the groups (T1, T2, T3), and no significant differences appeared compared to the negative control group (C1), but showed significant differences compared to the positive control group (C2). The positive control group (C2), which included animals that were given Moringa leaf extract, showed significant differences compared to the negative control group (C1).From the current study, we conclude that the Moringa leaf extract plays an important positive role in reducing the harmful effects of some food additives.

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