The relationship between some electrolyte levels and MTHFR C667T gene polymorphism in premenopausal and postmenopausal osteoporosis Iraqi women
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: AL-Azzawie AF, Husain WN, Salih MH, AL-Assie AH. The relationship between some electrolyte levels and MTHFR C667T gene polymorphism in premenopausal and postmenopausal osteoporosis Iraqi women. Eurasia J Biosci. 2020;14(1), 545-551.

APA 6th edition
In-text citation: (AL-Azzawie et al., 2020)
Reference: AL-Azzawie, A. F., Husain, W. N., Salih, M. H., & AL-Assie, A. H. (2020). The relationship between some electrolyte levels and MTHFR C667T gene polymorphism in premenopausal and postmenopausal osteoporosis Iraqi women. Eurasian Journal of Biosciences, 14(1), 545-551.

Chicago
In-text citation: (AL-Azzawie et al., 2020)
Reference: AL-Azzawie, Adnan F., Wasan N. Husain, Maan H. Salih, and Akeel H. AL-Assie. "The relationship between some electrolyte levels and MTHFR C667T gene polymorphism in premenopausal and postmenopausal osteoporosis Iraqi women". Eurasian Journal of Biosciences 2020 14 no. 1 (2020): 545-551.

Harvard
In-text citation: (AL-Azzawie et al., 2020)
Reference: AL-Azzawie, A. F., Husain, W. N., Salih, M. H., and AL-Assie, A. H. (2020). The relationship between some electrolyte levels and MTHFR C667T gene polymorphism in premenopausal and postmenopausal osteoporosis Iraqi women. Eurasian Journal of Biosciences, 14(1), pp. 545-551.

MLA
In-text citation: (AL-Azzawie et al., 2020)
Reference: AL-Azzawie, Adnan F. et al. "The relationship between some electrolyte levels and MTHFR C667T gene polymorphism in premenopausal and postmenopausal osteoporosis Iraqi women". Eurasian Journal of Biosciences, vol. 14, no. 1, 2020, pp. 545-551.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: AL-Azzawie AF, Husain WN, Salih MH, AL-Assie AH. The relationship between some electrolyte levels and MTHFR C667T gene polymorphism in premenopausal and postmenopausal osteoporosis Iraqi women. Eurasia J Biosci. 2020;14(1):545-1.

Abstract

Gene polymorphism related to osteoporosis plays a significant role in the development of osteoporosis, therefore, this study aimed to detect the MTHFR C667T gene polymorphism and evaluate its relationship with biochemical parameters in Iraqi women with premenopausal and postmenopausal osteoporosis in Salah Al-din province. Blood samples were collected from one hundred women (53 premenopausal and 47 postmenopausal) with osteoporosis diagnosed by specialist doctors and seventy healthy women with identical ages. Serum calcium and phosphorus concentrations were estimated and genomic DNA was extracted that used to detected MTHFR C667T polymorphism employing Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS-PCR) technique. The results showed a significant decrease in the calcium and phosphorus concentrations (p-value <0.001) for the osteoporosis group compared with controls. Frequency of T allele and TT genotype were high significant (p-value < 0.001) in osteoporosis patients compared with controls. But there were no significant differences in the frequency of genotypes and alleles between patients group. On the other hand, there was no correlation between the decrease of calcium and phosphorus levels and presence of T allele in the genotypes of patients (p-value > 0.05). Conclude that calcium and phosphorus levels and frequency of normal CC genotype were decreased while the frequency of mutant TT genotype was higher in women with osteoporosis compared to healthy women, this indicates that TT genotype and T allele can be considered as an indicator of osteoporosis in Iraqi women.

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