The relationship between placental gene polymorphisms and preeclampsia risk in Russian women

Abstract

To study the associations of polymorphisms of the NDRG1 gene, which is differentially expressed in the placenta, with the risk of preeclampsia (PE) development. The study group included 997 women: 366 pregnant women with preeclampsia and 631 women with physiological pregnancy. Clinical and laboratory examination of pregnant women was carried out in the Perinatal Center of the Belgorod Regional Clinical Hospital of St. Joasaph. DNA was isolated from peripheral venous blood lymphocytes by phenol-chloroform extraction. All women underwent typing of four single nucleotide polymorphisms of the N-myc downstream regulated gene 1 (NDRG1). The analysis of SNPs associations with the development of preeclampsia was performed using logistic regression analysis within the framework of additive, dominant and recessive genetic models. The average age in the group with preeclampsia was 0.75 years higher compared to the control group (p = 0.01). Among pregnant women with PE, body weight and body mass index exceeded those of the control group (p = 0.0001). Also, in this group, the percentage of women with obesity is 2.26 times higher (p = 0.001). The analysis of risk factors for PE revealed a higher incidence of arterial hypertension before pregnancy and a history of preeclampsia in the patient group as compared with the control (p = 0.0001). Compared with the control group, women with PE have a greater number of pregnancies (1.32 times, p = 0.03) due to a greater number of stillbirths (p = 0.02), miscarriages (0.01), and artifactual abortions (p = 0.0005). The rs12678229 NDRG1 allele A was found to be associated with the development of preeclampsia in the framework of the recessive model (OR = 1.46, 95% Cl 1.01-2.12, p = 0.046). The associations of other studied polymorphic markers with the development of preeclampsia were statistically insignificant. Thus, the rs12678229 NDRG1 allele is a risk factor for the development of preeclampsia among the women of the Central Black Earth Region of Russia.

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