The relationship between expression of Mir30 and Let7 genes in infertile males and non-obstructive azoospermia
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 1, pp. 393-398
  • Published Online: 10 May 2019
  • Article Views: 38 | Article Download: 22
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Mashizy SK, Shariati M, Mokhtari M, Khatamsaz S. The relationship between expression of Mir30 and Let7 genes in infertile males and non-obstructive azoospermia. Eurasia J Biosci. 2019;13(1), 393-398.

APA 6th edition
In-text citation: (Mashizy et al., 2019)
Reference: Mashizy, S. K., Shariati, M., Mokhtari, M., & Khatamsaz, S. (2019). The relationship between expression of Mir30 and Let7 genes in infertile males and non-obstructive azoospermia. Eurasian Journal of Biosciences, 13(1), 393-398.

Chicago
In-text citation: (Mashizy et al., 2019)
Reference: Mashizy, Saeed Khosravi, Mehrdad Shariati, Mokhtar Mokhtari, and Saeed Khatamsaz. "The relationship between expression of Mir30 and Let7 genes in infertile males and non-obstructive azoospermia". Eurasian Journal of Biosciences 2019 13 no. 1 (2019): 393-398.

Harvard
In-text citation: (Mashizy et al., 2019)
Reference: Mashizy, S. K., Shariati, M., Mokhtari, M., and Khatamsaz, S. (2019). The relationship between expression of Mir30 and Let7 genes in infertile males and non-obstructive azoospermia. Eurasian Journal of Biosciences, 13(1), pp. 393-398.

MLA
In-text citation: (Mashizy et al., 2019)
Reference: Mashizy, Saeed Khosravi et al. "The relationship between expression of Mir30 and Let7 genes in infertile males and non-obstructive azoospermia". Eurasian Journal of Biosciences, vol. 13, no. 1, 2019, pp. 393-398.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Mashizy SK, Shariati M, Mokhtari M, Khatamsaz S. The relationship between expression of Mir30 and Let7 genes in infertile males and non-obstructive azoospermia. Eurasia J Biosci. 2019;13(1):393-8.

Abstract

Any defect in the process of spermatogenesis can lead to a type of male infertility disorder called Non-Obstructive Azoospermia. Investigating factors involved in spermatogenesis, including genes, can help to understand the mechanism of infertility in men. On the other hand, a number of miRRNAs communicate with spermatogenesis and regulate it. The current paper aims at determining the let7 and mir30 genes expressions in testicular tissue of Azoospermic patients. This case-control study was performed on 40 infertile males with Non-Obstructive Azoospermia and 40 healthy fertile males. Genomic RNA from the testicular tissue samples as well as semen of the fertile individuals was extracted and converted to CDNA. Using Real-time PCR technique, using the specific primer of the examined genes and primer U6 as internal control, the expression rate in samples and controls was evaluated, and the amount of ΔCT was calculated, and finally, the relative number of multiplied mRNA copies of formula 2 -ΔΔCT was used. Data were then analyzed using Excel software, and compared using SPSS software and t-test and ANOVA. Based on the quantitative results obtained from the examination of the let7 and mir30 genes expression, the results showed that the let7 and mir30 gene expression in Azoospermic individuals was significantly different from that of fertile male sperm samples. The mean of let7 expression in Azoospermic individuals was more than 18 times higher than normal samples. (P-value = 0.04). There was a significant difference in expression of let7 in fertile and Azoospermic male samples by t-test. (P-value = 0.03). There was no significant difference in the expression of mir30 in fertile and infertile males with Azoospermia. (P-value = 0.27). There is a significant difference in the expression of let7 gene in Azoospermia samples compared to fertile individuals, and it can be concluded that this gene may play a role in the spermatogenesis process. By examining larger populations and proving this change of expression, the miRNA expression patterns were used to diagnose and treat Non-obstructive Azoospermic individuals.

References

  • Akoz A, Yildiz V, Orun S, Turkdogan KA, Duman A (2018) Management of Poisonous Snake Bites: Analysis of 29 Cases. J Clin Exp Invest., 9(4): 140-4. https://doi.org/10.5799/jcei/3998
  • Aslani F, Modarresi MH, Soltanghoraee H, Akhondi MM, Shabani A, Lakpour N, Sadeghi MR (2011) Seminal molecular markers as a non-invasive diagnostic tool for the evaluation of spermatogenesis in non-obstructive azoospermia. Systems biology in reproductive medicine, 57(4): 190-6. https://doi.org/10.3109/19396368.2011.569906
  • Barad O, Meiri E, Avniel A, Aharonov R, Barzilai A, Bentwich I, Einav U, Gilad S, Hurban P, Karov Y, Lobenhofer EK, Sharon E, Shiboleth YM, Shtutman M, Bentwich Z, Einat P (2004) MicroRNA expression detected by oligonucleotide microarrays: system establishment and expression profiling in human tissues. Genome Res., 14: 2486–94. https://doi.org/10.1101/gr.2845604
  • Brehm R, Zeiler M, Ruttinger C, Herde K, Kibschull M, Winterhager E, Willecke K, Guillou F, Lecureuil C, Steger K, et al. (2007) A sertoli cell-specific knockout of connexin43 prevents initiation of spermatogenesis. Am J Pathol, 171: 19-31. https://doi.org/10.2353/ajpath.2007.061171
  • Ezeh UIZO (2000) Beyond The clinical Classification of azoospermia. Hum Reprod., 15: 2356-9. https://doi.org/10.1093/humrep/15.11.2356
  • Ferrás C, Zhou XL, Sousa M, Lindblom A, Barros A (2007) DNA mismatch repair gene hMLH3 variants in meiotic arrest. Fertil Steril, 88(6): 1681-4. https://doi.org/10.1016/j.fertnstert.2007.01.063
  • Hackstein JHP, Hochstenbach R, Pearson PL (2000) Towards an understanding of the genetics of human male infertility: lesson from files. Trends in Genetics, 16(12):565-72. https://doi.org/10.1016/S0168-9525(00)02140-5
  • Hajkova P, Erhardt S, Lane N, Haaf T, ElMaarri O, Reik W, et al. (2002) Epigenetic reprogramming in mouse primordial germ cells. Mech Dev, 117(1-2): 15-23. https://doi.org/10.1016/S0925-4773(02)00181-8
  • Hamada A, Esteves SC, Nizza M, Agawal A (2012) Unexplained male infertility; diagnosis and management. Int braz J urol, 38(5): 576-94. https://doi.org/10.1590/S1677-55382012000500002
  • Ikuerowo SG, Izeybu MC, Benebo AS, Fadeyibi IO, Omodele FO (2010) Testicular Biopseis of Azoospermic Men at the Lagos State University Teaching Hospital. African journal of Urology, 16(3): 69-72. https://doi.org/10.1007/s12301-010-0016-5
  • Kilitci A, Kaya Z, Acar EM, Elmas ÖF (2018) Scrotal Calcinosis: Analysis of 5 Cases. J Clin Exp Invest., 9(4): 150-3. https://doi.org/10.5799/jcei/4002
  • Köseoğlu D, Karcı AÇ, Acar A, Tuna EE, Berker D (2018) Progressive severe dyspnea and hypoxia due to primary thyroid lymphoma: steroid administration may be life-saving. Electronic Journal of General Medicine, 15(5): em77. https://doi.org/10.29333/ejgm/94011
  • Kotaja N, Bhattacharyya SN, Jaskiewicz L, Kimmins S, Parvinen M, Filipowicz W, Sassone-Corsi P (2006) The chromatoid body of male germ cells: similarity with processing bodies and presence of Dicer and microRNA pathway components. Proc Natl Acad Sci U S A., 103: 2647–52. https://doi.org/10.1073/pnas.0509333103
  • Koudehi FA, Farazmand R, Mirzayian S, Mohamad S (2014) Customer Value Assessment Methodology Using DM Approach. UCT Journal of Research in Science, Engineering and Technology, 2(3).
  • Krausz C, Forti G (2000) Clinical aspects of male infertility. Result probl cell Diffcr, 28: 1-21. https://doi.org/10.1007/978-3-540-48461-5_1
  • Lian J, Zhang X, Tian H, Liang N, Wang Y, Liang C, et al. (2009) Altered microRNA expression in patients with non-obstructive azoospermia. Reprod Biol Endocrinol, 7: 13. https://doi.org/10.1186/1477-7827-7-13
  • Liu W, Niu Z, Li Q, Pang RT, Chiu PC, Yeung WS (2016) MicroRNA and embryo implantation. American Journal of Reproductive Immunology, 75(3): 263-71. https://doi.org/10.1111/aji.12470
  • Madison-Villar MJ, Michalak P (2011) Misexpression of testicular microRNA in sterile Xenopus hybrids points to tetrapod-specific microRNAs associated with male fertility. Journal of molecular evolution, 73(5-6): 316-24. https://doi.org/10.1007/s00239-011-9478-8
  • Magro VM (2015) An Unclear Anemia in an Elderly Subject with Aortic Stenosis. European Journal of General Medicine, 12(2): 170-3. https://doi.org/10.15197/sabad.1.12.35
  • Marcon E, Babak T, Chua G, Hughes T, Moens PB (2008) miRNA and piRNA localization in the male mammalian meiotic nucleus. Chromosome Research, 16(2): 243-60. https://doi.org/10.1007/s10577-007-1190-6
  • Mardani M, Lavasani SM, Omidvari M (2014) An investigation into DOW and MOND indices with fuzzy logic based on fire and explosion risk assessment in Iran oil refinery. UCT Journal of Research in Science, Engineering and Technology, 2(3): 126-37.
  • McIver SC, Stanger SJ, Santarelli DM, Roman SD, Nixon B, McLaughlin EA (2013) A unique combination of male germ cell miRNAs coordinates gonocyte differentiation. PloS one, 7(4): e35553. https://doi.org/10.1371/journal.pone.0035553
  • Moncada S, Palmer RM, Higgs EA (1991) Nitric oxide: Physiology, Pathophysiology and Pharmacology, Pharm. Rew., 43(2): 109-142.
  • Nuti F, Krausz C (2008) Gene polymorphisms/mutations relevant to abnormal spermatogenesis. Reprod Biomed Online, 16(4): 504-13. https://doi.org/10.1016/S1472-6483(10)60457-9
  • Rowe PJ, Comhaire FH, Hargreave TB, Mahmoud AMA (2000) WHO manual for standardized investigation, diagnosis and management of the infertile male. Cambridge: Cambridge University Press: 33.
  • Salas-Huetos A, Blanco J, Vidal F, Mercader JM, Garrido N, Anton E (2014) New insights into the expression profile and function of micro-ribonucleic acid in human spermatozoa. Fertility and sterility, 102(1): 213-22. https://doi.org/10.1016/j.fertnstert.2014.03.040
  • Stark A, Bushati N, Jan CH, Kheradpour P, Hodges E, Brennecke J, et al. (2008) A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands. Genes Dev, 22(1): 8-13. https://doi.org/10.1101/gad.1613108
  • Sutovsky P, Schatten G (1999) Paternal contributions to the mammalian Zygote: fertilization after sperm-egg fusion International review of cytology, 195: 1-65. https://doi.org/10.1016/S0074-7696(08)62703-5
  • Thonneau P, Marchand S, Tallec A, Ferial ML, Ducot B, Lansac J, et al. (19991) Incidence and main causes of infertility in a resident Population of three French regions (1988-1989), Hum. Reprod., 6(6): 811-6. https://doi.org/10.1093/oxfordjournals.humrep.a137433
  • Turner RM (2003) Tales from the tail: what do we reably know about sperm motility? J Androl., 24(6): 790-803. https://doi.org/10.1002/j.1939-4640.2003.tb03123.x
  • Wu W, Qin Y, Li Z, Dong J, Dai J, Lu C, Guo X, Zhao Y, Zhu Y, Zhang W, Hang B (2013) Genome-wide microRNA expression profiling in idiopathic non-obstructive azoospermia: significant up-regulation of miR-141, miR-429 and miR-7-1-3p. Human Reproduction, 28(7): 1827-36. https://doi.org/10.1093/humrep/det099
  • Yu JT, Tan L (2012) The role of clusterin in Alzheimer’s discase; Pathway, Pathogesis, and therapy. Mol Neurobial, 45(2): 314-26. https://doi.org/10.1007/s12035-012-8237-1

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