The RASA1 and Mir182 expression is reliable predictor for detection Acute Myeloid Leukemia
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Seyed Sharifi Kakhki AS, Onsory K. The RASA1 and Mir182 expression is reliable predictor for detection Acute Myeloid Leukemia. Eurasia J Biosci. 2019;13(2), 817-822.

APA 6th edition
In-text citation: (Seyed Sharifi Kakhki & Onsory, 2019)
Reference: Seyed Sharifi Kakhki, A. S., & Onsory, K. (2019). The RASA1 and Mir182 expression is reliable predictor for detection Acute Myeloid Leukemia. Eurasian Journal of Biosciences, 13(2), 817-822.

Chicago
In-text citation: (Seyed Sharifi Kakhki and Onsory, 2019)
Reference: Seyed Sharifi Kakhki, Akram Sadat, and Khadijeh Onsory. "The RASA1 and Mir182 expression is reliable predictor for detection Acute Myeloid Leukemia". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 817-822.

Harvard
In-text citation: (Seyed Sharifi Kakhki and Onsory, 2019)
Reference: Seyed Sharifi Kakhki, A. S., and Onsory, K. (2019). The RASA1 and Mir182 expression is reliable predictor for detection Acute Myeloid Leukemia. Eurasian Journal of Biosciences, 13(2), pp. 817-822.

MLA
In-text citation: (Seyed Sharifi Kakhki and Onsory, 2019)
Reference: Seyed Sharifi Kakhki, Akram Sadat et al. "The RASA1 and Mir182 expression is reliable predictor for detection Acute Myeloid Leukemia". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 817-822.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Seyed Sharifi Kakhki AS, Onsory K. The RASA1 and Mir182 expression is reliable predictor for detection Acute Myeloid Leukemia. Eurasia J Biosci. 2019;13(2):817-22.

Abstract

Introduction: Acute myeloid leukemia (AML) is a common type of acute leukemia in adults. The RAS pathway dysregulation is one of the most common routes of the cancers and RASA1 gene family plays a key role on RAS deactivation. The Mir182 is involved in cancer cells proliferation and survival. So, the aim of the current study was to determine the expression of RASA1 and Mir182 in patients with AML using Real time PCR and compare it with control group.
Materials and Methods: For this purpose, the blood samples were collected from 60 AML patients and 30 healthy individuals as control. The RNA extracted, quality and quantity of the RNA determined, cDNA synthesized and primers were designed. Then genes expression investigated using Real time PCR and ∆∆CT computational techniques. Results analyzed using GraphPad Prism 6.0 software.
Results: According to the results, the RASA1 gene expression significantly decreased in the AML patients compared to the normal group (P<0.0001), while the expression of Mir182 increased in the AML compared to the healthy people (P<0.0001). No significant correlation was observed between age, sex, WBC, PLT, HGB, Blast and FAB subtype with the disease (P> 0.05).
Conclusion: These results suggested elevated expression of the Mir182 might suppress the RASA1 gene expression. So, determining of the RASA1 and Mir182 levels might be a useful indicator for prognosis of the AML.

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