Calloselasma rhodostoma (Kuhl, 1824) is one species of Indonesian medically important snake that distributed in many Indonesia regions. With the recent snakebite cases of this snake and warrant of further improvement on anti-venin in Indonesia, the information on storage conditions for its venom is important for developing an anti-venin. The genetic identity that possibly holds a cryptic diversity that has not been resolved, could impact the future of anti-venin development of this species. We analyze the molecular data based on the ND4 gene to resolve the genetic relations of this snake from Java, Kangean, and Borneo population with the addition of the Thailand population by Bayesian Inference phylogenetic reconstruction. To evaluate the storage conditions, venom collection from six living specimens from the Java population was used to analyze the effect of svPLA2 activity under different storage conditions for 14 days long. The phylogenetic results show a polytomy tree, with a low p-distance value between populations. Only the storage at 37 ºC affects the performance of svPLA2 significantly. The phylogenetic indicating a single species even though divided by geographical barriers, more genes need to be compared to resolve the genetic relationship. More samples are needed to compare the venom properties throughout C. rhodostoma wide distribution, to enlight the anti-venin future development.

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