The effects of low-energy laser light in increasing the number of fibroblasts during the healing process of tendon achilles rupture in white rats (Rattus Norvegicus)

Abstract

Background: The recovery process of tendon injury is slower and results in weaker scar tissue. The increase in metabolism and the formation of new blood vessels in the tendon occurs in the tendon healing mechanism. Laser energy that hits the tissue increases the blood flow and lymph flow in the tissue. Purpose: This study aims to determine the effects of low energy laser therapy on the tendon healing process. Method: A total of 21 white rats were divided into three groups, i.e., G1 as the control group without additional therapy, G2 with the therapy in the inflammatory phase (day 3), and G3 with the therapy in the proliferative phase (day 8). G2 and G3 received the therapy for five days at a dose of 1 Joule. The experiment animals were terminated on the 21st day for evaluation, and the obtained and collected data at the end of the study were compared and analyzed using ANOVA statistical tests. Results: The groups that received the treatment using a low energy laser experienced an increase in the average number of fibroblasts by 78.99% per visual field in G2, and 79.23% in G3 in comparison to the control group. Conclusion: The administration of low energy laser therapy to the Achilles tendon healing of white rats brings positive results with an increase in the average number of fibroblasts.

References

  • Bjordal, J. M., Couppe, C., & Ljunggren, A. E. (2001). Low level laser therapy for tendinopathy. Evidence of a dose–response pattern. Physical Therapy Reviews, 6(2), 91-99.
  • Chen, C. H., Tsai, J. L., Wang, Y. H., Lee, C. L., Chen, J. K., & Huang, M. H. (2009). Low‐level laser irradiation promotes cell proliferation and mRNA expression of type I collagen and decorin in porcine achilles tendon fibroblasts in vitro. Journal of Orthopaedic Research, 27(5), 646-650.
  • Ernawati, D. S., & Puspa, A. (2018). Expression of vascular endothelial growth factor and matrix metalloproteinase-9 in Apis mellifera Lawang propolis extract gel-treated traumatic ulcers in diabetic rats. Veterinary world, 11(3), 304.
  • Gomez, M. (1995). The physiology and biochemistry of soft tissue healing. Rehabilitation of the injured knee, 34-44.
  • Juliastuti, W. S., Budi, H. S., & Maula, L. I. (2016). Fibroblast cell viability effectiveness between the highlands and lowlands coconut water (Cocos Nucifera L.). Journal of International Dental and Medical Research, 9(3), 237-241.
  • Lin, T. W., Cardenas, L., & Soslowsky, L. J. (2004). Biomechanics of tendon injury and repair. Journal of biomechanics, 37(6), 865-877.
  • Nascimento, R. X. D., & Callera, F. (2006). Low-level laser therapy at different energy densities (0.1–2.0 J/cm2) and its effects on the capacity of human long-term cryopreserved peripheral blood progenitor cells for the growth of colony-forming units. Photomedicine and Laser Therapy, 24(5), 601-604.
  • Nirwana, I., Rachmadi, P., & Rianti, D. (2017). Potential of pomegranate fruit extract (Punica granatum Linn.) to increase vascular endothelial growth factor and platelet-derived growth factor expressions on the post-tooth extraction wound of Cavia cobaya. Veterinary world, 10(8), 999.
  • Rhatomy, S., Arif, A., Utomo, D. N., Mulyadi, D., Salipi, M. B., Setyawan, R., & Soekarno, N. R. (2019). Posterior Cruciate Ligament reconstruction augmentation on avulsion PCL: A case series. International Journal of Surgery Open, 20, 15-19.
  • Shahabi, H., Salari, M., Ahmad, B. B., & Mohammadi, A. (2016). Soil Erosion Hazard Mapping in Central Zab Basin Using Epm Model in GIS Environment. International Journal of Geography and Geology, 5(11), 224-235.
  • Vo-Dinh, T. (2003). Handbook on Biomedical Photonics.

License

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.