The effects of hydro-alcoholic extract of zingiber officinale (ginger) on PPARγ gene expression, the blood lipid profiles and the liver enzymes (AST, ALT) in the high-fat diet-fed adult male rats


Zingiber officinale has been used as herbal medicine to treat various disorders. This study investigates the effect of hydro-alcoholic extract Zingiber officinale on the expression of hepatic Peroxisome proliferator-activated receptor-γ (PPARγ), the blood lipid profiles and the liver aspartate aminotransferase, alanine aminotransferase in the high-fat diet-fed adult male rats.Thirty-two rats were randomly divided into four groups (n=8 in each group). Two groups of rats were fed a standard diet whereas the other two groups were given a high-fat diet (HFD) for 8 weeks; Control group, high-fat diet group, hydro-alcoholic extract of Z. officinale (ZO) group and hydro-alcoholic extract of Z. officinale (ZO) + high-fat diet group were treated 400 mg/ of the extract by oral gavage once daily for 4 weeks. Gene expression, the lipid profile of serum and also AST, ALT were determined. The expressions of hepatic PPARγ was down-regulated (P < 0.0001) by ZO extract. The extract of ginger showed a significant decrease in triglycerides (P < 0.05), the levels of liver AST , ALT (P < 0.05) and a significant increase in serum High-density lipoprotein (HDL) cholesterol (P < 0.05) . However, no significant decrease in serum Low-density lipoprotein (LDL) and total cholesterol was observed either with both extract ginger groups compared to the control group. The results show that ginger extract may be responsible for the regulatory effects on PPARγ pathway. Moreover, the hydro-alcoholic extract of Zingiber officinale is capable of improving lipid disorders caused by the high-fat diet.


  • Ahd K, Dhibi S, Akermi S, Bouzenna H, Samout N, Elfekib A, Hfaiedh N (2019) Protective effect of ginger (Zingiber officinale) against PCB-induced acute hepatotoxicity in male rats. RSC Adv; 9(3): 29120-29130.
  • Aibara D, Matsuo K, Yamano S, Matsusue K (2019) Fat-specific protein 27b is regulated by hepatic peroxisome proliferator-activated receptor γ in hepatic steatosis, 19(6): 296-310.
  • Akinyemi A, Oboh G, Ademiluyi A, Boligon A, Athayde M (2016) Effect of Two Ginger Varieties on Arginase Activity in Hypercholesterolemic Rats. J Acupunct Meridian Stud; 9(2): 80-87.
  • Amirabadizadeh A, Foaddodini M, Khatamsaz S, Moktari M, Shariati M, Hosseini M, Hajipour F (2017) Introduction and Optimization of a Dietary Model for Inducing Hyperlipidemia in Rats. Journal of Babol University of Medical Sciences; 19(7): 35-41.
  • Arabloua T, Aryaeianb N (2018) The effect of ginger (Zingiber Officinale) as an ancient medicinal plant on improving blood lipids. Herbal Medicines Journal, 12(4): 11-15.
  • Attyah A, Ismail S (2012) Protective Effect of Ginger Extract Against Cisplatin-Induced Hepatotoxicity and Cardiotoxicity in Rats, Iraqi Journal of pharmaceutical Sciences, 21(1): 27-33.
  • De Las Heras N, Valero-Muñoz M, Martín-Fernández B, Ballesteros S, López-Farré A, Ruiz-Roso B, Lahera V (2017) Molecular factors involved in the hypolipidemic- and insulin-sensitizing effects of a ginger (Zingiber officinale Roscoe) extract in rats fed a high-fat diet. Applied Physiology, Nutrition, and Metabolism; 42(2): 209-215.
  • Dugasania S, Pichikac M, Nadarajahc VD, Balijepalli MK, Tandraa S, Korlakuntab JN (2010) Comparative antioxidant and anti-inflammatory effects of [6]-gingerol, [8]-gingerol, [10]-gingerol and [6]-shogaol. Journal of Ethnopharmacology; 12(7): 515-520.
  • ElRokh S, Yassin N, El-Shenawy S, Ibrahim B (2010) Antihypercholesterolemic effect of ginger rhizome (Zingiber officinale) in rats. Inflammopharmacology; 18(1): 309-315.
  • Elseweidy M, Younis N, Elswefy S, Abdallah F (2015) El-Dahmy SI, Elnagar G, Kassem HM. Atheroprotective potentials of curcuminoids against ginger extract in hypercholesterolaemic rabbits. Nat Prod Res; 29(10): 961-971.
  • Friedewald W, Levy R, Fredrickson D (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without the use of the preparative ultracentrifuge. Clinical Chemistry; 18(6): 499-502.
  • Gavrilova O, Haluzik M, Matsusue K, Cutson J, Johnson L, Dietz K, et al. (2003) Liver peroxisome proliferator-activated receptor gamma contributes to hepatic steatosis, triglyceride clearance, and regulation of body fat mass. J Biol Chem; 278(36): 34268-34276.
  • Han Q, Yuan Q, Meng X, Huo J, Bao Y, Xie G (2017) 6-Shogaol attenuates LPS-induced inflammation in BV2 microglia cells by activating PPAR-γ. Oncotarget; 8(26): 42001-42006.
  • Handa S, Khanuja Suman Longo G, Rakesh D (2008) Extraction technologies for medicinal and aromatic plants. International centre for science and high technology, 15(2): 21-25.
  • Helal E, El-Wahab S, Sharaf A, Zedan G (2012) Effect of Zingiber officinale on fatty liver induced by oxytetracycline in albino rats. The Egyptian Journal of Hospital Medicine; 46(4): 26-42.
  • Janani C, Ranjitha Kumari B (2015) PPAR gamma gene-A review, diabetes and metabolic syndrome; 9(1): 46-50.
  • Khosravani M, Azarbayjani M, Abolmaesoomi M, Yusof A, et al. (2016) Ginger extract and aerobic training reduces lipid profile in high-fat fed diet rats. Eur Rev Med Pharmacol Sci.; 20(8): 1617-1622.
  • Li C, Zhou L (2015) Inhibitory effect 6-gingerol on adipogenesis through activation of the Wnt/β-catenin signaling pathway in 3T3-L1 adipocytes, Toxicol in Vitro; 30(6): 394-401.
  • Liu C, Rajasekaran R, Lin S, Wang S, Kuo C, Tseng Y, Sheen L (2013) Metabolomics of Ginger Essential Oil against Alcoholic Fatty Liver in Mice. Journal of Agricultural and Food Chemistry; 61(46): 11231-11240.
  • Manaharan T, Kanthimathi M (2016) Ginger oil-mediated down-regulation of adipocyte-specific genes inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes. Biochemistry and Biotechnology Research; 4(2): 38-47.
  • Morgan K, Uyuni A, Nandgiri G, Mao L, et al. (2008) Altered expression of transcription factors and genes regulating lipogenesis in liver and adipose tissue of mice with high fat diet-induced obesity and nonalcoholic fatty liver disease. European Journal of Gastroenterology and Hepatology; 20(14): 843-854.
  • Pettinelli P, Videla L (2011) Up-Regulation of PPARγ mRNA Expression in the Liver of Obese Patients: an Additional Reinforcing Lipogenic Mechanism to SREBP-1c Induction. The Journal of Clinical Endocrinology and Metabolism; 96(5):1424-1430.
  • Rahimlou M, Yari Z, Rayyani E, Keshavarz S, Hosseini S, Morshedzadeh N, Hekmatdoost A (2019) Effects of ginger supplementation on anthropometric, glycemic and metabolic parameters in subjects with metabolic syndrome: A randomized, double-blind, placebo-controlled study.J Diabetes Metab Disord; 18(1): 119-125.
  • Sabina E, Pragasam S, Kumar S, Rasool M (2011) 6-gingerol, an active ingredient of ginger, protects acetaminophen-induced hepatotoxicity in mice. Journal of Chinese Integrative Medicine; 9(11): 1264-1269.
  • Salehi K, Kordlu A, Rezapour-Nasrabad R (2020) Prevalence of Type 2 Diabetes in Population Over 30 Years Old (2017-2018). Ethno Med;14(1-2): 24-29.
  • Schmittgen T, Livak K (2008) Analyzing real-time PCR data by the comparative CT method. Nature Protocols; 3(5): 1101-1108.
  • Srinivasan K (2017) Ginger rhizomes (Zingiber officinale): A spice with multiple health beneficial potentials. PharmaNutrition; 5(2): 18-28.
  • Villegas PJ (2016) Preliminary Study of Activated Carbon Filters for Pollutants Removal in Diesel Engines. International Journal of Sustainable Energy and Environmental Research, 5(2): 31-45.
  • Wang J, Li D, Wang P, Hu X, Chen F (2019) Ginger prevents obesity through regulation of energy metabolism and activation of browning in high-fat diet-induced obese mice. J Nutr Biochem; 70(2): 105-115.
  • Yi J, Ryoo Z, Ha J, Oh D, Kim M, Kim S (2019) Beneficial effects of 6‑shogaol on hyperglycemia, islet morphology, and apoptosis in some tissues of streptozotocin‑induced diabetic mice. Diabetology and Metabolic Syndrome; 11: 11-15.
  • Zhang Y, Hernandez-Ono A, Siri P, Weisberg S, Conlon D, et al. (2006) Aberrant hepatic expression of PPARgamma2 stimulates hepatic lipogenesis in a mouse model of obesity, insulin resistance, dyslipidemia, and hepatic steatosis. The Journal of Biological Chemistry; 281(49): 37603-37615.


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