The effect of zinc oxide nanoparticles on blood parameters and immunoglobulin A in male rats with diarrhea induced

Abstract

Objective: The present study was designed to detect the ability of ZnO NPs to inhibit the bacterial isolates that cause diarrhea in laboratory male rats and determination the blood parameters, liver enzyme functions and IgA levels after treatment with ZnO NPs. Material and methods: Twenty rats of 6 weeks-old male were used and divided as follow: Group 1: Control group that were not treatments. Group 2: The animals were infected with 1.2×10ᵔ8 of Clostridium difficile, (Infected group). Group 3: The rats were infected with C. difficile and orally dosage with 150mg/kg from ZnO NPs for two weeks. Results: The results showed that the orally dosage from C. difficile was causing the significant (P<0.05) decreased in RBCs, Hemoglobin concentration (Hb), HCT, and significant increase of WBCs count, neutrophil and decreased the lymphocytes count cells. Also, it was caused in significantly increased of ALT, AST and ALP enzymes in serum and IgA levels compare with same values in control group. the orally dosage from ZnO NPs was done all parameters were come at similar of the same parameters in control group. Conclusion: It’s concluded that ZnO NPs has ability to potential inhibition activity against C. difficile that induced diarrhea.

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