The effect of green tea extract to angiogenesis in bone regeneration


Background: Polyphenols contained in tea have anti-angiogenesis effects. Epigallocatechin-3-gallate (EGCC) extracted from green tea is a strong inhibitor of neutrophil-mediated angiogenesis, both in vitro and in vivo. Some studies have shown that oral or IP injection of ECGC could be a strong angiogenesis inhibition. Purpose: This study aims to determine the effect of green tea extract on angiogenesis in bone fracture healing. Method: The study utilized the Randomized Control Group Post Test-Only Design research. In the first draft, the sample was divided into several groups, i.e., K1 as a control group, and P1, P2, P3, and P4 as treatment groups. For the first draft, the experimental animals were terminated on the 10th day. The termination was undertaken on the 14th day for the second draft. This study utilized the Anova test to analyze the data. Results: The recommended dose for administering the green tea extract was 20 mg (p=0.447), then there would be a significant reduction (p=0.034) of cell numbers that represented VEGF if the dose was 25 mg or more. A significant reduction of blood vessel numbers (p=0.009) was indicated after administering 25 mg or more. Conclusion: The cross-sectional area of ​​blood vessels decreases when transferring 20 mg of the extract, while the blood vessels and cells number that represented VEGF decrease when administering 25 mg of the extract. The blood vessels contained in the callus also decrease after injecting 25 mg of green tea extract.


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