Aloe Vera is a medicinal plant with anti-inflammatory and anti-microbial effects. There is not enough data regarding application of Aloe Vera as storage media for periodontal ligament (PDL) preservation. The aim of this study was to evaluate the effects of this material at different concentrations and different time points on PDL cell vitality and apoptosis. In this study PDL cells were treated at different concentrations of Aloe Vera (25%, 50% and 100%), HBSS and water in- vitro. Cell Vitality was studied by Tetrazolium-based colorimetric MTT assay at 2, 6,12, 24 and 48 hours. Apoptosis assay was performed at 12, 24 and 48 hours. There was no significant difference in the number of viable and apoptotic cells in 100% Aloe Vera group compared to HBSS. The minimum number of viable cells and maximum number of apoptotic cells were observed in cells exposed to 25% Aloe Vera.


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