Toxoplasma gondii an intracellular parasite that cause toxoplasmosis. Toxoplasma infection it’s well known to induce various physiological, hormonal and behavioral changes in humans and animals. In current study, 147 infertile women were divided into three groups, primary, secondary infertility and aborted woman (sub-infertility). Twenty healthy volunteer women served as controls. Enzyme linked immunofluorescence assay was used to confirm anti-Toxoplasma IgG antibodies. Whereas, Testosterone concentration were measured by ELISA technique. We investigated the testosterone and total antioxidant capacity (TAC) in free and infected (Toxoplasma gondii) infertile women, to provide clinical scenario that may be adopted as one causes of primary, secondary infertility and sub infertility. Although, there are no significant variation in Testosterone levels between infertility women groups, but according normal, hypo and hyper range, testosterone were increased significantly just in toxoplasmosis women(p-value=0.04), whereas no significant variation are seen in other infertile groups. Testosterone statistically decreased in primary infertile women. In additions, serological analysis confirm that 28 (19%) enrolled women were seropositive for Toxoplasma gondii. Toxoplasmosis have a significant impact on Total antioxidant capacity especially in secondary infertility and sub-infertility. In addition no significant variations were reached in TAC and Testosterone as correlated with intensity of parasites (anti-IgG antibodies) .interestingly the testosterone hyper concentration are found in secondary infertile women(p-value=0.01, 29%) and in opposite direction percentage of hypo-testosterone is increased significantly in primary infertility women . In conclusion this study suggested a considerable evidence of hypothesis that consider Toxoplasmosis is a one of a risk factor for increase the level of Testosterone and increasing the chance of infertility in women especially according Hypo-Hyper values. Also based our data that supplementations of TAC is recommended for women who had primary and secondary infertility to enhance reproductive outcome.

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