The antidiabetic effect of bitter melon (Momordica charantia L.) extracts towards glucose concentration, langerhans islets, and leydig cells of hyperglycemic mice (Rattus norvegicus)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Meles DK, Wurlina, Anom Adnyana DP, Rinaldhi CP, Octaviani RR, Sekar Cempaka DK. The antidiabetic effect of bitter melon (Momordica charantia L.) extracts towards glucose concentration, langerhans islets, and leydig cells of hyperglycemic mice (Rattus norvegicus). Eurasia J Biosci. 2019;13(2), 757-762.

APA 6th edition
In-text citation: (Meles et al., 2019)
Reference: Meles, D. K., Wurlina, Anom Adnyana, D. P., Rinaldhi, C. P., Octaviani, R. R., & Sekar Cempaka, D. K. (2019). The antidiabetic effect of bitter melon (Momordica charantia L.) extracts towards glucose concentration, langerhans islets, and leydig cells of hyperglycemic mice (Rattus norvegicus). Eurasian Journal of Biosciences, 13(2), 757-762.

Chicago
In-text citation: (Meles et al., 2019)
Reference: Meles, Dewa Ketut, Wurlina, Dewa Putu Anom Adnyana, Chaterina Puspadewanti Rinaldhi, Rian Rizky Octaviani, and Desak Ketut Sekar Cempaka. "The antidiabetic effect of bitter melon (Momordica charantia L.) extracts towards glucose concentration, langerhans islets, and leydig cells of hyperglycemic mice (Rattus norvegicus)". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 757-762.

Harvard
In-text citation: (Meles et al., 2019)
Reference: Meles, D. K., Wurlina, Anom Adnyana, D. P., Rinaldhi, C. P., Octaviani, R. R., and Sekar Cempaka, D. K. (2019). The antidiabetic effect of bitter melon (Momordica charantia L.) extracts towards glucose concentration, langerhans islets, and leydig cells of hyperglycemic mice (Rattus norvegicus). Eurasian Journal of Biosciences, 13(2), pp. 757-762.

MLA
In-text citation: (Meles et al., 2019)
Reference: Meles, Dewa Ketut et al. "The antidiabetic effect of bitter melon (Momordica charantia L.) extracts towards glucose concentration, langerhans islets, and leydig cells of hyperglycemic mice (Rattus norvegicus)". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 757-762.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Meles DK, Wurlina, Anom Adnyana DP, Rinaldhi CP, Octaviani RR, Sekar Cempaka DK. The antidiabetic effect of bitter melon (Momordica charantia L.) extracts towards glucose concentration, langerhans islets, and leydig cells of hyperglycemic mice (Rattus norvegicus). Eurasia J Biosci. 2019;13(2):757-62.

Abstract

Purpose: This study was aimed to prove the fruit extract of bitter melon (Momordica charantia L.) towards blood sugar levels, of Langerhans islets cells, and Leydig cells of hyperglycemic white mice (Rattus norvegicus).
Methods: Twenty-five mice were divided randomly into 5 groups. They all were induced intraperitoneally by alloxan with dosage of 150 mg/kg in order to damage to the pancreas. From all treatments group, three groups (P1, P2, and P3) were treated with various doses of bitter melon extract with dosage 29, 50, and 59 mg/1 ml/day, respectively. As a comparative group, –negative control group (P0 +) were given with CMC-Na 0,5% 1ml/day, whereas the positive control group (K+) were given Glibenclamide® 0.126 mg/1 ml/day. Bitter melon extract was given for 21 days. In the first day of treatment, blood glucose level of mice was examined after 2 hours, 4 hours, 6 hours, 8 hours after treatments. The blood glucose examinations were subsequently continued at days 7th, 14th and 21st after treatment. After 21 days, the pancreas and testes of mice were taken for histopathological preparations made.
Results: Bitter melon (Momordica charantia L.) extract had antidiabetic effects that can lower blood glucose level, improved pancreatic beta cell damage, and increased the Leydig cells number in a dosage of 50 mg/1 ml/day on the 21st days after treatment.
Conclusion: the extract of bitter melon fruit (Momordica charantia L.) at a dosage of 50 mg/kg/1ml/day can lower blood glucose levels and increased the number of Langerhans islets and Leydig cell of hyperglycemia mice.

References

  • Ayoub SM, Rao S, Byregowda SM, Mayasandra, Satyanarayana L, Bhat N, Shridhar NB, Shridhar PB (2013) Evaluation of hypoglycemic effect of Momordica charantia extract in distilled water in streptozotocin-diabetic rats. Braz J Vet Pathol, 6(2): 56-64.
  • Ballester J, Munoz C, Dominguez J, Rigau T, Guinovart JJ, Gil JER (2004) Insulin dependent diabetes affects testicular function by FSH and LH-linked mechanisms. Int J Androl, 7: 243-257. https://doi.org/10.1002/j.1939-4640.2004.tb02845.x
  • Dellmann HD, Carithers JR (1996) Cytology and microscopic anatomy. Baltimore: Williams & Wilkins: 457–467.
  • Halliwell B, Gutteridge JMC (2007) Free radicals in biology and medicine. 4th edition. New York: Oxford University Press: 142-151.
  • Larry A, Blume CA, Cavallerano J, Beste BD, Pederson J, Walls LL (2009) Optometric clinical practice guideline care of the patient with diabetes mellitus. USA: American Optometric Association.
  • Mayes PA (2002) Metabolism asam lemak tak jenuh dan eicosanoid. Jakarta: Sikumbang EGC: 242-259.
  • Meles DK, Wurlina, Anom Adnyana IDP (2017) Measurement of Alkaloids Achyranthes Aspera Linn Level Using Thin Layer Chromatography Method and High-Performance Liquid Chromatography. The Veterinary Medicine International Conference 2017. KnE Life Sciences, pp. 378–385. https://doi.org/10.18502/kls.v3i6.1146
  • Meles DK, Wurlina, Nian (2009) Kadar glukosa darah setelah pemberian alloxan pada tikus putih (Rattus norvegicus). J Veterinary, 5(4): 34.
  • Pitteloud N, Hardin M, Dwyer AA, Valassi E, Yialamas M, Elahi D, et al. (2005) Increasing insulin resistance is associated with a decrease in Leydig cell testosterone secretion in men. J Clin Endocrinol Metab, 90(5): 2636-2641. https://doi.org/10.1210/jc.2004-2190
  • Rho H, Lee JT, Kim HR, Pard BH (2000) Protective mechanism of glucose against alloxan-induced β-cell damage: pivotal role of ATP. Exp Mol Med, 32(1): 12-17. https://doi.org/10.1038/emm.2000.3
  • Subahar, Tati SS, Lentera T 2004() Khasiat dan manfaat pare si pahit pembasmi penyakit. Jakarta: Agromedia Pustaka.
  • Szkudelski T (2008) The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res, 50(6): 537-546.
  • Watkins D, Cooperstein SJ, Lazarow A (1964) Effect of alloxan on the permeability of pancreatic islet tissue in vitro. Am J Physiol, 207: 436-440. https://doi.org/10.1152/ajplegacy.1964.207.2.436
  • Winarsi H (2007) Antioksidan alami dan radikal bebas. Yogyakarta: Penerbit Kanisius.
  • Wurlina, Meles DK, Mustofa I, Zakaria S, Anom Adnyana IDP (2017) Alkaloid immunomodulatory effects of sambiloto (Andrographis paniculate L.) On the response of gamma interferon and t helper cell (cd4+). Advances in Natural and Applied Sciences, 11(9): 154-158.
  • Wurlina, Meles DK, Zakaria S, Mustofa I, Susilowati S, Anom Adnyana IDP (2017) Acute Toxicity Tests of Alkaloid Pare (Momordica charantia) Fruit on The Histopathology of Liver. The Veterinary Medicine International Conference 2017, KnE Life Sciences, pp. 588–695. https://doi.org/10.18502/kls.v3i6.1186
  • Yeum KJ, Beretta G, Krinsky NI, Russell RM, Aldini G (2009) Synergistic interactions of antioxidant nutrients in a biological model system. Nutrition, 25(7-8): 839-846. https://doi.org/10.1016/j.nut.2009.01.011

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