Synthesis and characterization of poly (3-hydroxybutyrate) films containing mentha piperita oil and antimicrobial activity assay by diffusion method

Abstract

Food decomposition, spoilage and corruption caused by food borne microorganisms and non-biodegradable plastic materials for food packaging are the most important problems that threaten the environment and the economy. As a result, aim this study producing packaging materials with durable antibacterial properties against food spoilage microorganisms. To keep food fresh and a healthy environment. The effectiveness of this new active packaging material against four spoilage bacteria isolated from garden soil was tested by Mueller Hinton agar -diffusion method. A circles were cut from the film with approximately 0.6 cm in diameter and tested. The FTIR spectra of Mentha piperita oil with those of Poly (3-hydroxybutyrate) (P(3HB)) and it was possible to observe the characteristic bands and through which can be checked incorporation of the Mentha piperita oil into the polymer matrix can be verified. Microbiological analyzes showed that (P(3HB)) film with 10 % of oil better antimicrobial activity to Staphylococcus aureus with the inhibition zone 17.4 mm. Salmonella spp was more sensitive compared to Escherichia coli and Bacillus cereus with inhibition zones diameters 12.8, 8.5 and 5.6 mm, respectively. Through the previous results obtained in this study is a first step in the manufacture of cans and bags have the ability to biodegradation and antibacterial properties of microorganisms to obtain an environment add and free of waste.

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