Background: Chronic Hepatitis C (CHC) disease is known as a main public health problem through the world. CHC disease might lead to fibrosis of hepatocytes. In present paper, we attempted to measure whether there is any association among IL-35 and CLU with MAD, GSH, CAT and SOD, and several biochemical variables such as AST, ALT, GGT, ALP, T. BIL, and albumin. Methods: This study has included 44 patients from the Al-Fallujah Teaching Hospital, who was having CHC disease, and 44 healthy controls (HCs) were registered. Serum AST, ALT, GGT, ALP, T. BIL, albumin concentrations were estimated by colorimetric methods, IL-35 and CLU, MAD, GSH, CAT and SOD levels were estimated by ELISA technique. Results: Compared with healthy controls, the serum IL-35 and CLU, GSH, CAT and SOD concentrations were lower of patients with CHC and the serum MDA values were higher (P<0.0001, all), the serum IL-35 and CLU concentrations related positively with serum GSH, CAT, SOD and albumin, and negatively correlated with MDA, AST, ALT, GGT, ALP and T. BIL concentrations (all P<0.01). The area under the receiver operating characteristic (AUROC) curve of serum for MDA, CLU, SOD, CAT, IL-35 and GSH levels were 0.9904, 0.9403, 0.9274, 0.9246, 0.8515 and 0.82, respectively. Conclusion: Correlation among IL-35 and CLU with MAD, GSH, CAT and SOD may be attractive markers to predict disease development in CHC patients.

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