Study the adhesion capacity on abiotic surfaces by acinetobacter baumannii isolated from drinking water
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 4217-4223
  • Published Online: 21 Oct 2020
  • Open Access Full Text (PDF)


Acinetobacter baumannii ability to form biofilm makes it to be opportunistic pathogen causing of nosocomial infections and to be good survivor in adverse environmental conditions including medical devices and hospital environments.
Six isolates of A. baumannii were isolated from drinking water and tested to investigate biofilm formation capacity on three different type of abiotic surface, also several factors were examined such as hydrophobicity, PH and temperature.
All A. baumannii isolates displayed a positive biofilm on congored aga test CRA (pigmented colonies with black color) and Christensen’s test (adhesive layer of stained material to the inside surface of the tube).The obtained data of microbial adhesion to hydrocarbons assay (MATH) assay revealed that the percentage of all isolates ranged between (45-75%).
Results of recent study revealed that optical density OD values were consistently higher on catheter than on that of the polystyrene and glass at any of the PH and temperature Temperature 37◦C and PH 4 have greatest positive effect on biofilm formation process than other values, Current study may help in additional understanding of A. baumannii ability to form biofilm on abiotic surface which may be is used in medical devices’ manufacturer and role of this in spreading of this pathogen in hospital environment.


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