Study of gentamicin combined with polyhydroxyalkanoates extracted from lactobacillus plantarum
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 2903-2910
  • Published Online: 16 Sep 2020
  • Open Access Full Text (PDF)

Abstract

Background: Polyhydroxyalkanoates (PHAs) are natural bio-polymers, which are formed by many micro-organisms in the context of a carbon and energy reservoir. PHAs are mainly dependent on the number of carbons and their molecular weight in the monomer unit. PHAs are biomedical products due to their biocompatible, biodegradable or non-toxic effects. Methodology: Nine bacterial isolates were isolated from oil-contaminated soil samples in different locations of Babylon province were diagnosed to Pseudomonas spp via traditional methods. In addition to three isolates of Lactobacillus bacteria obtained from the Advanced Microbiology Laboratory/ College of Science/ Babylon University, they were isolated from dairy. Results: all isolates were screened for PHA production by Sudan black B and Nile blue A. six isolates of Pseudomonas spp. and three of Lactobacillus spp. were shown positive for the production of PHA. After primary screening the polymer was extracted by sodium hypochlorite and chloroform. The best producing polymer in 3.4% from cell dry weight was found to be Lactobacillus spp1 was identified and characterized by VITEK 2 compact device; the result showed 87% identical to Lactobacillus plantarum. Fourier transform infrared spectroscopy FTIR was used to show the functional group of extracted polymer from L. plantarum, FT-IR was used to show the functional groups of PHA samples were identified as C-O, C-H and C=O. The FT-IR spectra of PHA containing carbonyl group (C=O) occurred in a strong band at 1739.85cm-1. The solubility of PHA was also measured in several solvents. The results showed that the polymer was well dissolved by chloroform and Dimethyl sulfoxide. The polymer produced from L. plantarum was combined with Gentamicin was added to obtain a 5:1 or 10:1 (w/w) Gentamicin content to improve the performance improvement of Gentamicin against bacterial biofilm. Diagnosed pathogenic bacteria were then obtained from hospitals in Babylon province for later use in Gentamicin and PHA loaded Gentamicin running tests. The minimum inhibitory concentration MIC of the Gentamicin concentration was 0.2 mg/ml. The antibacterial activity of Gentamicin, PHA loaded Gentamicin (5:1 or 10:1) and PHA was studied against two types of gram-positive (Enterococcus spp) and gram-negative (K. pneumoniae) bacteria; the results show the effect of PHA loaded Gentamicin (5:1 or 10:1) stronger than Gentamicin against pathogenic bacteria, while there is not any effect for the alone polymer.The biofilm formation was studied on a wavelength of 630 nm via the ELISA reader and it was found that both isolates can form biofilms. Conclusion: The study concluded that Polyhydroxyalkanoate loaded Gentamicin (1:5) was stronger than Gentamicin and Polyhydroxyalkanoate loaded Gentamicin (1:10) against the biofilm formation.

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