AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Boyko NN, Zhilyakova ET, Bondarev AV, et al. Study of antimicrobial activity and technology optimization of Calendulae flos galenicals. Eurasia J Biosci. 2019;13(2), 969-974.

APA 6th edition
In-text citation: (Boyko et al., 2019)
Reference: Boyko, N. N., Zhilyakova, E. T., Bondarev, A. V., Kazakova, V. S., Pisarev, D. I., Novikov, O. O., . . . Nefedova, L. V. (2019). Study of antimicrobial activity and technology optimization of Calendulae flos galenicals. Eurasian Journal of Biosciences, 13(2), 969-974.

Chicago
In-text citation: (Boyko et al., 2019)
Reference: Boyko, Nikolay N., Elena T. Zhilyakova, Alexander V. Bondarev, Valentina S. Kazakova, Dmitriy I. Pisarev, Oleg O. Novikov, Tatyana P. Osolodchenko, Rita V. Sahaidak-Nikitiuk, and Lilia V. Nefedova. "Study of antimicrobial activity and technology optimization of Calendulae flos galenicals". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 969-974.

Harvard
In-text citation: (Boyko et al., 2019)
Reference: Boyko, N. N., Zhilyakova, E. T., Bondarev, A. V., Kazakova, V. S., Pisarev, D. I., Novikov, O. O., . . . Nefedova, L. V. (2019). Study of antimicrobial activity and technology optimization of Calendulae flos galenicals. Eurasian Journal of Biosciences, 13(2), pp. 969-974.

MLA
In-text citation: (Boyko et al., 2019)
Reference: Boyko, Nikolay N. et al. "Study of antimicrobial activity and technology optimization of Calendulae flos galenicals". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 969-974.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Boyko NN, Zhilyakova ET, Bondarev AV, Kazakova VS, Pisarev DI, Novikov OO, et al. Study of antimicrobial activity and technology optimization of Calendulae flos galenicals. Eurasia J Biosci. 2019;13(2):969-74.

Abstract

The article presents materials on optimization of manufacture technology for Calendulae flos galenicals with medium level of antibacterial activity. For antibacterial study of extracts, we used agar well diffusion method. In our research, we utilized six test-strain microorganisms: E. coli ATCC 25922, S. aureus ATCC 25923, P. vulgaris ATCC 4636, P. aeruginosa ATCC 27853, C. albicans ATCC 885/653, and B. subtilis ATCC 6633. We have found that the maximum level of extracts’ antimicrobial activity is achieved in the range of ethanol content in the extractant from 70 to 97 % v/v. Basing on these results, we have suggested a highly effective filtration technology of extraction for manufacture of liquid extract and tincture with medium level of antimicrobial activity from Calendulae flos. Based on HPLC analysis of extracts we detected the following main groups of compounds: quercetin and caffeic acid of derivatives. We have found that the antimicrobial activity of galenicals have good correlation with dry residue concentration but not with the rutin and chlorogenic acid concentration.

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