Spectral characterization, antioxidant and anti-genotoxic properties of methanol and ethanol stem extracts of Cissus araloides hook

Abstract

Purpose: This study investigated the phytocomponents, functional groups, antioxidant and anti-genotoxic properties of methanol and ethanol stem extracts of Cissus araloides.
Method: The phytocomponents’ analyses of the extracts were determined using a gas chromatograph interfaced with a mass spectrometer (GC-MS) while functional groups were obtained using Fourier Transform Infra-red spectrometer (FT-IR). The antioxidant activity of the extracts was evaluated using ferric reducing antioxidant power (FRAP), 2, 2-diphenyl-l-picrylhydrazyl (DPPH), peroxidase activity, and 2, 2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Anti-genotoxicity activity of the extracts was carried out using Allium cepa injected with 20µ Lethidium bromide solution.
Results: The results indicated the presence of saponins, flavonoids, tannins, steroids and carbohydrate in the methanol and ethanol stem extracts of C. araloides 25 compounds were detected from methanol extract. The functional -OH and C=C groups revealed by FTIR was validated by GC-MS analysis to indicate significant amounts of Benzoic acid-2,6-dihydroxy-, Chenodiol, Estriol, Ginkgolide A, Oxazepam, PGF2α and Resorcinol-2-acetate, that is typical of polyphenolic compounds. The methanol extract showed peroxidase activity (IC50 = 490.07μg/ml), ABTS (IC50 = 89.19 μg/ml) and FRAP (IC50 = 441.43μg/ml). Significant decrease (p<0.5 was indicated in fragmented DNA level of Allium cepa roots growth in ethidium bromide treated with C. araloides stem extracts when compared with ethidium bromide control. However, fragmented DNA was lower in A. cepa roots growth in methanol extract when compared with ethanol extract. Hence, extracts of C.araloides stem can be viewed as a new perspective in developing antioxidant agent.
Conclusion: Therefore it is recommended that C. araloides be utilized in natural products to strengthen our health.

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