Separation of four fatty acids and two phenolic compounds from Camellia sinensis using chromatographic techniques and evaluation of their antibacterial activity

Abstract

The active components were separated of Camellia sinensis leaves, by column chromatography through solvent of (Petroleum ether - ethyl acetate) 5-1 for extract petroleum ether and (chloroform- Methanol) 10-1 of extract ethanol. The Saponification was performed on to isolated and identification of four fatty acids (Hiptanoic, Lauric, Oleic and Palmitic acids) by GLC-chromatography., some phenolic compounds were isolated with acidhydrolysis, like Thymol and Hydroquinone. These compounds was identified by HPLC-analysis. The active compounds separated in this study had multiple effects against some Gram-positive and Gram-negative bacteria, using disc diffusion method.

References

  • Adam PO (2006) Antibacterial activity of aqueous and ethanol extracts of the stem bark of Alsonia boonei and Morinda lucida. Sci. Res. Essaya, 1(2): 50-53.
  • Akowuah GA, Ismail Z, Norhayati L, Sadikun A (2005) The Effects of Different Extraction Solvents of Varying Polarities of Polyphenols of Orthosiphon stamineus and Evaluation of the Free Radical-Scavenging Activity. Journal of Food Chemistry, 93: 311-317.
  • Al-Dulayymi FI (2014) Separation and Identification of Many Natural Products from Some Flowers of Medicinal Plants and Their Biological and Synergistic effects with Isolated Phenazine from Pseudomonas aeruginosa Against Some Pathogenic Microorganism. Ph.D. Thesis, College of Education for Pure Science, University of Mosul, 51-56.
  • Al-Kaisy MT, Hadwan HA, Saleh HM, Hussen AK (1991) Determination of citric and oxalic acids in fermented solutions of Aspergillus niger by gas-liquid chromatography. Iraqi: J. Microbiol. 3: 170-177.
  • Anita P, Sivasamy S, Kumar PDM, Balan IN, Ethiraj S (2015) In vitro antibacterial activity of Camellia sinensis extract against Cariogenic microorganisms. Journal of Basic and Clinical Pharmacy Vol. 6, Issue 1, December – February.
  • Anuonye JC, CE C, Olukayode J, Suleiman A (2016) Nutrient Composition, Amylose Content and Pasting Characteristics of Some Elite Accessions of Nerica Rice. Journal of Food Technology Research, 3(1): 36-47.
  • Aruther IV (1972) Practical organic chemistry including qualitative organic analysis 3rd edition, 445.
  • Asif M (2011) Health effects of omega 3.6.9 fatty acids: perilla frutesceus is a good example of plant oil orient oharm. Exp. Med. 11(1): 51-59.
  • Bauer AW, Kirby WMM, Sherns JC, Turch M (1966) Antibiotic susceptibility testing by a standardized single disc method. Am. J. Clin. Pathol. 45: 493-496.
  • Boran H, Ciftci C, Er A, Kose O, Kurtoglu IZ, Kaisy S (2005) Evaluation of Antibacterial activity of Green Tea (Camellia sinensis L.) Seeds Against Some Fish Pathogens in Rainbow Trout (Oncorhynchus mykiss, Walbaum).
  • Camargo LEA, Pedroso LS, Vendrame SC, Mainardes RM, Khalil NM (2016) Antioxidant and antifungal activities of Camellia sinensis (L.) Kuntze Leaves obtained by different forms of production. https://doi.org/10.1590/1519-6984.18814
  • Catherine AF (2007) Medicinal Plants: A Botanic Garden for the Nation. Botanical. Garden, 121-165 Tropical Biomedicine, 22(2): 165-170.
  • Falleh H, Ksouri R, Chaieb K, Karras-Bouraoni N, Trabelsi N, Boullaaba M, Abdelly C (2008) Phenolic Composition of Cynara Cardunculus L. organs and their biological activities C.R. Biologies, 331: 372-374.
  • George KO, Wanyoko JK, Kinyaniju T, Moseti KO, Wechira FN (2016) Comparative Assessment of the fatty acid profiled of Crude Oils Extracted from Seeds of Selected Tea (Camellia sinensis L.) Cultivars http://www.scip.org/journal/fns
  • Harborne JB (1998) Phytochemical Methods, London: Chapman and Hall, 60-66.
  • Kahlel AMS, Sultan FI (2019) Response of four potato cultivars to soil application with organic and amino acid compounds. Res. On Crops, Printed in India. 20(1): 101-108.
  • Keller AC, Ryan EP (2013) Antibacterial activity and phytochemical profile of fermented Camellia sinensis (Fuzhuan tea). Food Research International 53(2): 945-949.
  • Khalel AMS, Sultan FI (2019) Influence of foliar spray and soil application of three amino acids compounds in growth and yield of onion (Allium cepa L.). Plant Archives, 19(1): 531-534.
  • Kopjar M, Tadic M, Pilizota V (2015) Phenol content and antioxidant activity of green, yellow and black tea leaves. Chemical and Biological Technologies in Agriculture, 2:1 https://doi.org/10:1186/s40538-0028-7
  • Nitiema LW, Savadogo A, Simpore J, Diamon D, Traore AS (2012) In Vitro Antimicrobial Activity of some Phenolic Compounds (Coumarin and Queretin) Against Gastroenteritis Bacterial Strains. International Journal of Microbiological Research. 3(3): 183-187.
  • Padmini E, Valarmathi A, Usha RM (2011) Comparative analysis of chemical composition and antibacterial activities of Mentha spicata and Camellia sinesis. Asian J. Exp. Biol. Sci. 4: 772-781.
  • Robinson EE, Maxwell SRJ, Thorpe GHG (1997) An investigation of the antioxidant activity of black tea using enhanced chemiluminescence. Free Radial Research, 26: 291-302.
  • Setyopratomo P (2014) Extraction of phenolic compounds from green tea using ethanol. ARPN Journal of Engineering and Applied Sciences 9(9), September.
  • Sharangi AB (2009). Medical and therapeutic potentialities of tea (Camellia sinensis L.) – A review. Food research international 42: 529-535.
  • Tariq AL, Nirjontha D, Reyaz AL (2013) Antimicrobial activity of Camellia sinensis Leaves against gram positives and gram negative bacteria. World Research Journal of pharmaceutical Research. 1(1): 01-04.
  • Tariq AL, Reyaz AL (2013) Antioxidant activity of Camellia sinensis Leaves. Int. J. Curr. Microbiol. App. Sci. 2(5): 40-46.
  • Ushimaru PI, Mariama TN, Di Luciano D, Ary FJ (2007) Brazilian Journal of Microbiology, 38: 717-719.
  • Wang Y, Sun D, Chen H, Oian L, Xu P (2011) Fatty acid composition and Antixiodant Activity of Tea (Camellia sinensis L.) Seed Oil extracted by optimized supercritical carbon Dioxide. Int. J. Mol. Sci. 12: 7708-7719.
  • Wang ZY, Huang Mt, Chang R, Ma W, Ferrars T (1992) Inhibitory effect of green tea on the growth of established skin papillomas in mice. Cancer Research, 1;52(23): 6657-6665.
  • Yildrim A, Mavi A, Oktay M, Aydan A, Omer K, Algur F, Bibloglu A (2000) Comparison of Antioxidant and Antimicrobial Activities of (Tilia Argentea Dest Ex Du), sage (Satvia Triloba L.) and black tea (Camellia sinensis) Extracts. J. Agric. Food. Chem., 48(10): 5030-5034.
  • Zhou Q, Chen Z, Li JX, Amancio WSS (Editor) (2017) Proteomic analysis of tea plants (Camellia sinensis) with purple young shoots during leaf development. J. of Plos One, 12(5). PMC: 5433784.

License

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.