Separation of four fatty acids and two phenolic compounds from Camellia sinensis using chromatographic techniques and evaluation of their antibacterial activity


The active components were separated of Camellia sinensis leaves, by column chromatography through solvent of (Petroleum ether - ethyl acetate) 5-1 for extract petroleum ether and (chloroform- Methanol) 10-1 of extract ethanol. The Saponification was performed on to isolated and identification of four fatty acids (Hiptanoic, Lauric, Oleic and Palmitic acids) by GLC-chromatography., some phenolic compounds were isolated with acidhydrolysis, like Thymol and Hydroquinone. These compounds was identified by HPLC-analysis. The active compounds separated in this study had multiple effects against some Gram-positive and Gram-negative bacteria, using disc diffusion method.


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