Rho-kinase inhibitor Y-27632 on Schlemm’s canal diameter in a juvenile rat model injected with sodium hyaluronate
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 2, pp. 791-794
  • Published Online: 07 Jul 2019
  • Article Views: 168 | Article Download: 79
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Vrieda WV, Wulandari LR, Effendi MM, Sujuti H. Rho-kinase inhibitor Y-27632 on Schlemm’s canal diameter in a juvenile rat model injected with sodium hyaluronate. Eurasia J Biosci. 2019;13(2), 791-794.

APA 6th edition
In-text citation: (Vrieda et al., 2019)
Reference: Vrieda, W. V., Wulandari, L. R., Effendi, M. M., & Sujuti, H. (2019). Rho-kinase inhibitor Y-27632 on Schlemm’s canal diameter in a juvenile rat model injected with sodium hyaluronate. Eurasian Journal of Biosciences, 13(2), 791-794.

Chicago
In-text citation: (Vrieda et al., 2019)
Reference: Vrieda, Wino Vierlia, Lely Retno Wulandari, M. Ma’sum Effendi, and Hidayat Sujuti. "Rho-kinase inhibitor Y-27632 on Schlemm’s canal diameter in a juvenile rat model injected with sodium hyaluronate". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 791-794.

Harvard
In-text citation: (Vrieda et al., 2019)
Reference: Vrieda, W. V., Wulandari, L. R., Effendi, M. M., and Sujuti, H. (2019). Rho-kinase inhibitor Y-27632 on Schlemm’s canal diameter in a juvenile rat model injected with sodium hyaluronate. Eurasian Journal of Biosciences, 13(2), pp. 791-794.

MLA
In-text citation: (Vrieda et al., 2019)
Reference: Vrieda, Wino Vierlia et al. "Rho-kinase inhibitor Y-27632 on Schlemm’s canal diameter in a juvenile rat model injected with sodium hyaluronate". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 791-794.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Vrieda WV, Wulandari LR, Effendi MM, Sujuti H. Rho-kinase inhibitor Y-27632 on Schlemm’s canal diameter in a juvenile rat model injected with sodium hyaluronate. Eurasia J Biosci. 2019;13(2):791-4.

Abstract

Background: The purpose of this study was to examine the effects of the rho-kinase inhibitor Y-27632 on the diameter of the Schlemm’s canal in a juvenile rat model injected with sodium hyaluronate.
Material and Methods: This was an original experimental study with a post-test control group design. Eyeballs of rats aged 4-6 months were used in this study. Samples were divided into six groups: negative control, positive control I with intracameral sodium hyaluronate injection, positive control II with topical Y-27632 10 mM, and three experimental groups with intracameral injections of sodium hyaluronate and Y-27632 10-1 mM, 1 mM, and 10 mM, respectively. Changes of the Schlemm’s canal diameter were evaluated. Quantitative measurements were taken using computerized image analysis with the dot slide program.
Results: There were statistically significant differences among the control and experimental groups (p < 0.05). The greatest increase in diameter of the Schlemm’s canal was observed in the experimental group given sodium hyaluronate and Y-27632 10 mM, with a mean value of 118.42 µm.
Conclusion: The rho-kinase inhibitor Y-27632 increased the diameter of the Schlemm’s canal in juvenile rats injected with sodium hyaluronate.

References

  • Acot TS, Kelley MJ (2008) Extracellular Matrix in the Trabecular Meshwork. Exp Eye Res, 86(4): 543–561. https://doi.org/10.1016/j.exer.2008.01.013
  • Benozzi J, Nahum LP, Campanelli JL, Rosenstein RE (2002) Effect of Hyaluronic Acid on Intraocular Pressure in Rats. Invest Ophthalmol Vis Sci, 43(7): 2196-2200.
  • Festing MF, Altman DG (2002) Guidelines for the Design and Statistical Analysis of Experiments Using Laboratory Animals. ILAR J, 43(4): 244-258. https://doi.org/10.1093/ilar.43.4.244
  • Gasiorowzki JZ, Russell P (2009) Biological properties of trabecular meshwork cells. Exp Eye Res, 88(4): 671–675. https://doi.org/10.1016/j.exer.2008.08.006
  • Honjo M, Tanihara H, Inatani M, Kido N, Sawamura T, Yue BY, Narumiya S, Honda Y (2001) Effects of Rho-Associated Protein Kinase Inhibitor Y-27632 on Intraocular Pressure and Outflow Facility. Invest Ophthalmol Vis. Sci, 42(1): 137-144.
  • Ishizaki T, Uehata M, Tamechika I, Keel J, Nonomura K, Maekawa M, Narumiya S (2000) Pharmacological Properties of Y-27632, a Specific Inhibitor of Rho-Associated Kinases. Mol Pharmacol, 57(5): 976–983.
  • Kameda T, Inoue T, Inatani M, Fujimoto T, Honjo M, Kasaoka N, Inoue-Mochita M, Yoshimura N, Tanihara H (2012) The Effect of Rho-associated Protein Kinase Inhibitor on Monkey Schlemm’s Canal Endothelial Cells. Invest Ophthalmol Vis Sci, 53(6): 3092-3103. https://doi.org/10.1167/iovs.11-8018
  • Rao PV, Deng PF, Kumar J, Epstein DL (2001) Modulation of Aqueous Humor Outflow Facility by the Rho-Kinase Specific Inhibitor Y-27632. Invest Ophthalmol Vis Sci, 42(5): 1029-1037.
  • Rhee DJ, Haddadin RI, Kang MH, Oh DJ (2009) Matricellular proteins in the trabecular meshwork. Exp Eye Res, 88(4): 694–703. https://doi.org/10.1016/j.exer.2008.11.032
  • Shen X, Koga T, Park BC, SundarRaj N, Yue BY (2008) Rho GTPase and cAMP/Protein Kinase A Signaling Mediates Myocillin-induced Alterations in Cultured Human Trabecular Meshwork Cells. J Biol Chem, 283(1): 603-612. https://doi.org/10.1074/jbc.M708250200
  • Tamm ER (2009) The trabecular meshwork outflow pathways: Structural and functional aspects. Exp Eye Res, 88(4): 648–655. https://doi.org/10.1016/j.exer.2009.02.007
  • Temming K, Fretz MM, Kok RJ (2008) Organ and Cell-Type Specific Deliver of Kinase Inhibitors: A Novel Approach in the Development of Targeted Drugs. Curr Mol Pharmacol, 1(1): 1-12. https://doi.org/10.2174/1874-470210801010001
  • Tian B, Gabelt BT, Geiger B, Kaufman PL (2009) The Role of the Actomyosin System in Regulating Trabecular Fluid Outflow. Exp Eye Res, 88(4): 713–717. https://doi.org/10.1016/j.exer.2008.08.008
  • Yu WY, Sheridan C, Grierson I, Mason S, Kearns V, Lo ACY, Wong D (2011) Progenitors for the Corneal Endothelium and Trabecular Meshwork: A Potential Source for Personalized Stem Cell Therapyin Corneal Endothelial Diseases and Glaucoma. J Biomed Biotechnol: 1-13. https://doi.org/10.1155/2011/412743

License

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.