Relationship between multi-drug resistance and extended-spectrum β-latamase genes of Acinetobacter baumannii in different wards at Basra Hospitals
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 2963-2968
  • Published Online: 16 Sep 2020
  • Open Access Full Text (PDF)

Abstract

Background: The present study was aimed for determination the prevalence of Extended- Spectrum β- Lactamase (ESBL) genes from Acinetobacter baumannii isolates obtained from hospital-acquired infection at Basra hospitals and to determine the relationship ESBL genes with Multidrug Resistance (MDR) among these A. baumannii isolates. Methodology: A total 120 samples were collected from burn, urine, Intensive Care Unit (ICU) and pediatric wards at different Basra hospitals and these were identified depending on standard microbiological methods with VITEK-2 system. The high percentages were (31.5%) isolates of Staphylococcus aureus followed by pseudomonas aeruginosa (27.6%) and Acinetobacter baumannii (25.3%). Results: Furthermore, antimicrobial susceptibility test and Multi-Drug Resistance of A. baumannii were achieved according to CLSI method. It has been found that A. baumanii had high resistance that Cefotaxime (94%), Imipenem (91%), Cefoxitin (85%), Merpenem (82%), Gentamicin (76%), Aztreonam (73%), Ciprofloxacin (70%) and Ampicillin (61%). On the other hand, high percentage (84.6%) of multidrug resistance was observed in A. baumannii. In addition, phenotypic expression of ESBL- A. baumannii was demonstrated by Double Disc Synergy Test (DDST) method in (73%) isolates of MDR- A. baumanii . ESBL genes (bla- TEM, bla- SHV, bla- CTX) of these isolates were detected by Polymerase Chain Reaction (PCR) assay. The most frequent genes of A. baumannii were bla- TEM genes 21(64%), followed by bla-SHV genes 17(52%) and bla- CTX genes 14(42%). Conclusion: From this study, we concluded that significant relationship can be occurred between MDR isolates of A. baumannii with ESBL genes among environment of Basra hospitals.

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