Protective effect of methanol extract of Kelor (Moringa oleifera) leaves on Glutathione Peroxidase (GPx) levels in trabecular meshwork cell culture of primary congenital glaucoma patients
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Wulandari LR, Umiati S, Sujuti H. Protective effect of methanol extract of Kelor (Moringa oleifera) leaves on Glutathione Peroxidase (GPx) levels in trabecular meshwork cell culture of primary congenital glaucoma patients. Eurasia J Biosci. 2019;13(2), 839-844.

APA 6th edition
In-text citation: (Wulandari et al., 2019)
Reference: Wulandari, L. R., Umiati, S., & Sujuti, H. (2019). Protective effect of methanol extract of Kelor (Moringa oleifera) leaves on Glutathione Peroxidase (GPx) levels in trabecular meshwork cell culture of primary congenital glaucoma patients. Eurasian Journal of Biosciences, 13(2), 839-844.

Chicago
In-text citation: (Wulandari et al., 2019)
Reference: Wulandari, Lely Retno, Sri Umiati, and Hidayat Sujuti. "Protective effect of methanol extract of Kelor (Moringa oleifera) leaves on Glutathione Peroxidase (GPx) levels in trabecular meshwork cell culture of primary congenital glaucoma patients". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 839-844.

Harvard
In-text citation: (Wulandari et al., 2019)
Reference: Wulandari, L. R., Umiati, S., and Sujuti, H. (2019). Protective effect of methanol extract of Kelor (Moringa oleifera) leaves on Glutathione Peroxidase (GPx) levels in trabecular meshwork cell culture of primary congenital glaucoma patients. Eurasian Journal of Biosciences, 13(2), pp. 839-844.

MLA
In-text citation: (Wulandari et al., 2019)
Reference: Wulandari, Lely Retno et al. "Protective effect of methanol extract of Kelor (Moringa oleifera) leaves on Glutathione Peroxidase (GPx) levels in trabecular meshwork cell culture of primary congenital glaucoma patients". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 839-844.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Wulandari LR, Umiati S, Sujuti H. Protective effect of methanol extract of Kelor (Moringa oleifera) leaves on Glutathione Peroxidase (GPx) levels in trabecular meshwork cell culture of primary congenital glaucoma patients. Eurasia J Biosci. 2019;13(2):839-44.

Abstract

Background: The aim of this study was to determine differences in glutathione peroxidase (GPx) activity after multiple doses of methanol extract of Moringa oleifera leaves in a trabecular meshwork cell culture from primary congenital glaucoma patients.
Material and Methods: This was an experimental laboratory study; cell line cultures were divided into four groups (negative control and three treatment groups). The treatment groups were exposed to methanol extract of Moringa oleifera leaves at dosages of 15 µg/mL, 20 µg/mL, and 25 µg/mL. After four hours incubation, GPx activity was measured in all groups using a colorimetric microplate reader.
Results: ANOVA analysis found that there was a difference in GPx activity between treatment groups. Each Moringa oleifera extract dosage (15 µg/mL, 20 µg/mL, and 25 µg/mL) significantly differed from each other, with dosage 25 µg/mL having the highest GPx activity. The correlation analysis showed there was a significant association between exposure to Moringa oleifera extract and GPx activity with a positive correlation coefficient (r = 0.962, p < 0.005), which means higher dosages of Moringa oleifera extract will increase GPx activity. Linear regression analysis found thet every 1 mg/mL dosage of Moringa oleifera extract will increase GPx activity by 1.217 mU/mL.
Conclusion: It can be concluded that there were differences in GPx activity between treatment groups and there was an influence of dosage of methanol extract of Moringa oleifera leaves on GPx activity, such that increasing doses of methanol extract of Moringa oleifera leaves will further increase GPx activity.

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