Physiological study of the use of bull seminal plasma in skim milk diluent to improve quality of frozen ram semen
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Susilowati S, Wurlina, Anom Adnyana IDP, Mustofa I, Hariadi M. Physiological study of the use of bull seminal plasma in skim milk diluent to improve quality of frozen ram semen. Eurasia J Biosci. 2019;13(1), 193-197.

APA 6th edition
In-text citation: (Susilowati et al., 2019)
Reference: Susilowati, S., Wurlina, Anom Adnyana, I. D. P., Mustofa, I., & Hariadi, M. (2019). Physiological study of the use of bull seminal plasma in skim milk diluent to improve quality of frozen ram semen. Eurasian Journal of Biosciences, 13(1), 193-197.

Chicago
In-text citation: (Susilowati et al., 2019)
Reference: Susilowati, Suherni, Wurlina, I Dewa Putu Anom Adnyana, Imam Mustofa, and Mas’ud Hariadi. "Physiological study of the use of bull seminal plasma in skim milk diluent to improve quality of frozen ram semen". Eurasian Journal of Biosciences 2019 13 no. 1 (2019): 193-197.

Harvard
In-text citation: (Susilowati et al., 2019)
Reference: Susilowati, S., Wurlina, Anom Adnyana, I. D. P., Mustofa, I., and Hariadi, M. (2019). Physiological study of the use of bull seminal plasma in skim milk diluent to improve quality of frozen ram semen. Eurasian Journal of Biosciences, 13(1), pp. 193-197.

MLA
In-text citation: (Susilowati et al., 2019)
Reference: Susilowati, Suherni et al. "Physiological study of the use of bull seminal plasma in skim milk diluent to improve quality of frozen ram semen". Eurasian Journal of Biosciences, vol. 13, no. 1, 2019, pp. 193-197.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Susilowati S, Wurlina, Anom Adnyana IDP, Mustofa I, Hariadi M. Physiological study of the use of bull seminal plasma in skim milk diluent to improve quality of frozen ram semen. Eurasia J Biosci. 2019;13(1):193-7.

Abstract

Ram semen freezing process still generates poor outcome due to phospholipase. Thus, an alternative to substitute ram seminal plasma is recently developed using bull seminal plasma. The aim of this study was to determine the benefits of bull seminal plasma added by ram spermatozoa diluent in the freezing process. This study used control treatment (P0); (P1) was (diluent + ram semen) + bull seminal plasma (1: 1); (P2) was (diluent + ram semen) + bull seminal plasma (1:2). The result showed that P1 generated the highest viability, motility, plasma membrane intact, and the lowest DNA fragmentation, compared to second treatment and control. Statistical analysis of viability, motility, plasma membrane intact and DNA fragmentation were significantly different (p<0.05) among treatments. It can be concluded that additional of seminal plasma of bull can maintain motility, viability, plasma membrane intact and decrease the of DNA fragmentation in ram spermatozoa after-thawing.

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