Phylogenetic analysis of Giardia lamblia using small subunit ribosomal RNA(ssrRNA) gene and triose phosphates isomerase (TPI) gene isolated from Iraqi patients
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Alhatemi AKS, AlHuchaimi SN, Alshammari MMM, Bashbosh AE, Obaid RF. Phylogenetic analysis of Giardia lamblia using small subunit ribosomal RNA(ssrRNA) gene and triose phosphates isomerase (TPI) gene isolated from Iraqi patients. Eurasia J Biosci. 2020;14(1), 1127-1133.

APA 6th edition
In-text citation: (Alhatemi et al., 2020)
Reference: Alhatemi, A. K. S., AlHuchaimi, S. N., Alshammari, M. M. M., Bashbosh, A. E., & Obaid, R. F. (2020). Phylogenetic analysis of Giardia lamblia using small subunit ribosomal RNA(ssrRNA) gene and triose phosphates isomerase (TPI) gene isolated from Iraqi patients. Eurasian Journal of Biosciences, 14(1), 1127-1133.

Chicago
In-text citation: (Alhatemi et al., 2020)
Reference: Alhatemi, Abdulkareem Khattar Shaalan, Sundus Nsaif AlHuchaimi, Majida Malik Meteab Alshammari, Alia Essa Bashbosh, and Rasha Fadhl Obaid. "Phylogenetic analysis of Giardia lamblia using small subunit ribosomal RNA(ssrRNA) gene and triose phosphates isomerase (TPI) gene isolated from Iraqi patients". Eurasian Journal of Biosciences 2020 14 no. 1 (2020): 1127-1133.

Harvard
In-text citation: (Alhatemi et al., 2020)
Reference: Alhatemi, A. K. S., AlHuchaimi, S. N., Alshammari, M. M. M., Bashbosh, A. E., and Obaid, R. F. (2020). Phylogenetic analysis of Giardia lamblia using small subunit ribosomal RNA(ssrRNA) gene and triose phosphates isomerase (TPI) gene isolated from Iraqi patients. Eurasian Journal of Biosciences, 14(1), pp. 1127-1133.

MLA
In-text citation: (Alhatemi et al., 2020)
Reference: Alhatemi, Abdulkareem Khattar Shaalan et al. "Phylogenetic analysis of Giardia lamblia using small subunit ribosomal RNA(ssrRNA) gene and triose phosphates isomerase (TPI) gene isolated from Iraqi patients". Eurasian Journal of Biosciences, vol. 14, no. 1, 2020, pp. 1127-1133.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Alhatemi AKS, AlHuchaimi SN, Alshammari MMM, Bashbosh AE, Obaid RF. Phylogenetic analysis of Giardia lamblia using small subunit ribosomal RNA(ssrRNA) gene and triose phosphates isomerase (TPI) gene isolated from Iraqi patients. Eurasia J Biosci. 2020;14(1):1127-33.

Abstract

Giardia lamblia is most important intestinal protozoan worldwide. Giardiasis manifestation differ from asymptomatic infection to chronic diarrhea .The current study is performed to genotye G. lamblia isolates from Iraqi patients by molecular analysis. Ten hundred positive direct smear for giardiasis of different ages and sexes were exposed to nested polymerase chain reaction (PCR) for triose phosphates isomerase (TPI) and small subunit ribosomal RNA (ssrRNA) genes. The products of PCR were sequenced and the sequences were subjected to phylogenetic analysis by using multiple sequence alignment analysis of the partial triose phosphate isomerase (tpi)gene sequence in local Giardia lamblia genotypes isolates and NCBI-Genbank local Giardia lamblia genotypes based ClustalW alignment analysis by using (M`EGA 6.0, multiple alignment analysis tool). Only 80 samples gave positive results by molecular analysis. The phylogenetic analysis of the sequences was detected that assemblage A, B, mixed subtypes AB and F were isolated from stool samples. It was found that 18 (22.5%), 42 (52.5%), 8 (10% and 12(15%) corresponding to genotype A,B, AB, and F respectively. Phylogenetic tree analysis based on the partial sequence of triose phosphate isomerase (tpi) gene in local Giardia lamblia genotypes isolates that used for confirmative genetic detection and genetic relationship analysis. The evolutionary distances were computed using phylogenetic UPGMA tree type (MEGA 6.0 version). The local Giardia lamblia genotype AII were showed genetically closed related to NCBI-Blast Giardia lamblia genotype AII (KT235928.1), local Giardia lamblia genotype AI were showed genetically closed related to NCBI-Blast Giardia lamblia genotype AI (MF671916.1), local Giardia lamblia genotype F were showed genetically closed related to NCBI-Blast Giardia lamblia genotype F (KU378636.1), and local Giardia lamblia genotype B were showed genetically closed related to NCBI-Blast Giardia lamblia genotype B (JF918474.11 ) at total genetic change (0.05-0.15). A, B, and F were the genotypes that have a prevalence in the city of Najaf. assemblage B is the more prevalent subgroup compared with other assemblages. This is the first study that involved a phylogenetic analysis of Giardia strains and recorded assemblage F from Najaf patient. The present results provide to a better considerate of the molecular epidemiology of Giardia parasite.

Keywords

Giardia PCR Phylogeny

References

  • Al-Rubaie NEY (2019) Molecular detection of Giardia lamblia genotypes and virus among patients with giardiasis in Karbala province. MSc Thesis. College of Medicine University of Kufa Iraq.
  • Amar CF, Dear PH, Pedraza-Dı´az S, Looker N, Linnane E, McLauchlin J (2002) Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces. J Clin Microbiol 40: 446-452.
  • Anbia M, Masoomi S, Sedaghat S, Sepehrian M (2019) Potassium Halides-Impregnated Eggshell as a Heterogeneous Nanocatalysts for Biodiesel Production. Journal of Environmental Treatment Techniques, 7(1): 103-108.
  • Babaei Z, Oormazdi H, Akhlaghi L, Rezaie S, Razmjou E, Soltani AS SK (2008) Molecular characterization of the Iranian isolates of Giardia lamblia: application of the glutamate dehydrogenase gene. Iran J. Pub Health 37(2).
  • Caccio SM, Beck R, Lalle M, Marinculic A, Pozio E (2008) Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B. Int J Parasitol., 38: 1523-1531. https://doi.org/10.1016/j.ijpara.2008.04.008
  • Caccio` SM, Ryan U (2008) Molecular epidemiology of giardiasis. Mol Biochem Parasitol 160: 75-80.
  • Choy SH, Mahdy MA, Al-Mekhlafi HM, Low VL, Surin J (2015) Population expansion and gene flow in Giardia duodenalis as revealed by triosephosphate isomerase gene. Parasit Vectors. 2015; 8: 454. https://doi.org/10.1186/s13071-015-1084-y
  • Cooper MA, Adam RD, Worobey M, Sterling CR (2007) Population genetics provides evidence for recombination in Giardia. Curr Biol 17: 1984-1988. https://doi.org/10.1016/j.cub.2007.10.020
  • Dado D, Montoya A, Blanco MA, Miró G, Saugar JM, Bailo B, Fuente I (2012) Prevalence and genotypes of Giardia duodenalisfrom dogs in Spain: possible zoonotic transmission and public health importance. Parasitol Res, 111: 2419-2422. https://doi.org/10.1007/s00436-012-3100-x
  • Degerli S, Degerli N, Celiksoz A, et al. (2012) Genotyping of Giardia intestinalis isolated from people living in Sivas, Turkey. Turk J Med Sci. 42: 1268-1272.
  • Fantinatti M, Bello AR, Fernandes O, Da-Cruz AM (2016) Identification of Giardia lamblia Assemblage E in Humans Points to a New Anthropozoonotic Cycle. J Infect Dis, 214(8): 1256-1259. https://doi.org/10.1093/infdis/jiw361
  • Feng Y, Xıao L (2011) Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clin Microbiol Rev. 24: 110-140.
  • Helmy MM, Abdel-Fattah HS, Rashed L (2009) Real-time PCR/RFLP assay to detect Giardia intestinalis genotypes in human isolates with diarrhea in Egypt. J Parasitol.; 95: 1000-1004.
  • Heyworth MF (2016) Giardia duodenalis genetic assemblages and hosts. Parasite 23: 13. https://doi.org/10.1051/parasite/2016013
  • Hooshyar H, Ghafarinasab S, Arbabi M, Delavari M, Rasti S (2017) Genetic Variation of Giardia lamblia Isolates from Food-handlers in Kashan, Central Iran. Iranian journal of parasitology, 12(1): 83-89.
  • Islam SN, Tareq SAM (2015) In Vitro Cloning and Stem Cutting of Stevia (Stevia Rebaudiana Bertoni.) for Mass Propagation in Chittagong, Bangladesh. The International Journal of Biotechnology, 4(3): 14-19.
  • Molina N, Polverino D, Minvielle M, Basualdo J (2007) PCR amplification of triosephosphate isomerase geneof Giardia lamblia in formalin-fixed fec. Rev Latinoam Microbiol; 49(1-2): 6-11.
  • Monis PT, Andrews RH, Mayrhofer G, Ey PL (2003) Genetic diversity within the morphological species Giardia intestinalis and its relationship to host origin. Infect Genet Evol, 3: 29-38. https://doi.org/10.1016/s1567-1348(02)00149-1
  • Nader P, Hamidullah R, Mohammad RN, Hossein AY, Ahmad HS (2014). Molecular identification of Giardia lamblia; is there any correlation between diarrhea and genotyping in Iranian population, Gastroenterol Hepatol Bed Bench. Summer; 7(3): 168-172.
  • O’ Handley RM, Olson ME, Fraser D, Adams P, Thompson RC (2000) Prevalence and genotypic characterisation of Giardia in dairy calves from Western Australia and Western Canada. Vet Parasitol 90: 193-200.
  • Pestechian N, Rasekh H, Rostami-Nejad M, Yousofi HA, Hosseini-Safa A (2014) Molecular identification of Giardia lamblia; is there any correlation between diarrhea and genotyping in Iranian population?. Gastroenterology and hepatology from bed to bench, 7(3): 168-172.
  • Rasheed QA, M Jebur MS (2018) Molecular diversity and Phylogenetic tree of Giardia lamblia among some Iraqi gastrointestinal patients. J. Pharm. Sci. & Res. 10(7): 1701-1706.
  • Rayani M, Hatam G, Unyah NZ, Ashrafmansori A, Abdullah WO, Hamat RA (2017) Phylogenetic Analysis of Giardia lamblia Human Genotypes in Fars Province, Southern Iran. Iranian journal of parasitology, 12(4): 522-533.
  • Read C, Walters J, Robertson ID, Thompson RCA (2002) Correlation between genotype of Giardia duodenalis and diarrhoea. Int. J. Parasitol.; 32(2): 229-231.
  • Robertson LJ, Hanevik K, Escobedo AA, Mørch K, Langeland N (2010) Giardiasis–why do the symptoms sometimes never stop? Trends in Parasitology; 26: 75-82. https://doi.org/10.1016/j.pt.2009.11.010
  • Sahagún J, Clavel A, Goñi P, Gómez-Lus R (2008) Correlation between the presence of symptoms and the Giardia duodenalis genotype. Eur J Clin Microbiol Infect Dis, 27: 81-83. https://doi.org/10.1007/s10096-007-0404-3
  • Soliman RH, Fuentes I, Rubio JM (2011) Identification of a novel Assemblage B subgenotype and a zoonotic Assemblage C in human isolates of Giardia intestinalis in Egypt. Parasitol Int.; 60(4): 507-511. https://doi.org/10.1016/j.parint.2011.09.006
  • Sprong H, Caccio` SM, van der Giessen JW (2009) ZOOPNET network and partners: identification of zoonotic genotypes of Giardia duodenalis. PLoS Negl Trop Dis 3(12): e558. https://doi.org/10.1371/journal.pntd.0000558
  • Sung-Hee H, Anu D, Jeong Y, Abmed D, Cho SH, Lee WJ, Lee SE (2014) Molecular Characterization of Giardia duodenalis and Cryptosporidium parvum in Fecal Samples of Individuals in Mongolia. Am. J. Trop. Med. Hyg., 90(1): 43-47. https://doi.org/10.4269/ajtmh.13-0271
  • Tamer GS, Kasap M, Er DK (2015) Genotyping and phylogenetic analysis of Giardia duodenalis isolates from Turkish children. Medical science monitor: international medical journal of experimental and clinical research, 21: 526-532. https://doi.org/10.12659/MSM.892318
  • Torres-Romero JC, Euan-Canto A, Benito-González N, Padilla-Montaño N, Huchin-Chan C, Lara-Riegos J, Cedillo-Rivera R (2014) Intestinal parasites and genotyping of Giardia duodenalis in children: first report of genotype B in isolates from human clinical samples in Mexico. Memorias do Instituto Oswaldo Cruz, 109(3): 388-390. https://doi.org/10.1590/0074-0276140507
  • Tsourdi E, Heidrich FM, Winzer M, Röllig C, Kirsch C, Meinel J, Hofbauer LC (2014). An exotic cause of exudative enteropathy. Am J Case Rep., 15: 226-229. https://doi.org/10.12659/AJCR.890483
  • Turki NM, Mallah MO, Kremsh YD (2015) Iraqi Genotyping of Giardia lambila (A,B,E,F) in Human Stool In AL-Muthanna Provinc–Iraq. International Journal of Advanced Research, 3(10): 757-771.
  • Wegayehu T, Karim MR, Li J, Adamu H, Erko B, Zhang L, Tilahun G (2016) Multilocus genotyping of Giardia duodenalis isolates from children in Oromia Special Zone, central Ethiopia. BMC microbiology, 16: 89. https://doi.org/10.1186/s12866-016-0706-7
  • Yahya WH, Yousif JJ (2013) A diagnostic Study Of The Giardia Lamblia Parasite Genotypes, Which Causes Diarrhea Among The Patients In Najaf, Province, By Using The PCR Technique. J Kufa for Nursing Science, 3(2): 1-10.

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