Phylogenetic analysis of acne Staphylococci highlights their diversity
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 1, pp. 1973-1981
  • Published Online: 30 Jun 2020
  • Open Access Full Text (PDF)

Abstract

Acne is usually an inflammation of skin which Staphylococcus is one causative agents of it. Therefore, the detection of species belonging to the staphylococcal acne and the extent of its diversity is the aim of this study. Based on the amplification and sequencing of 16S rRNA gene, the phylogenetic relationships of staphylococci isolated from acne were constructed using many bioinformatics tools. The results of alignment showed all isolates belonged to Staphylococcus genus subdivided to five species included 6 (46.2%) S. epidermidis, 4 (30.7%) S. aureus, 1 (7.7%) S. haemolyticus, 1(7.7%) S. warneri and 1 (7.7%) S. lentus. The highest divergence (more than 3%) was found between S. lentus and other studied species whereas the lowest divergence (0.84%) was between S. epidermidis and S. warneri. The first three species found to be closest to acne isolates in GenBank while S. warneri and S. lentus were to our knowledge is the first recorded case as causative agent for acne. In conclusion, the 16S rRNA sequencing of pathogenic staphylococci isolated from acne was good method to reveal its diversity and identify other new species have ability to cause acne which it need farther investigation to clarify their role in this type of skin inflammation.

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