Phenotypic detection and vancomycin MICs for methicillin resistance Staphylococcus aureus isolated from nebulizer
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 1, pp. 2153-2161
  • Published Online: 30 Jun 2020
  • Open Access Full Text (PDF)


Nebulizer and other respiratory care devices became as reservoir of pathogens when colonized with microorganisms and play important role in transmitted from patient to an another, Methicillin resistance Staphylococcus aureus is one of the main pathogens caused nosocomial infection in particular hospital respiratory infections. during a 6- month period 52 swab samples were collected from contaminated nebulizer masks, all samples were cultivated on mannitol salt agar then chromogenic agar (CHROMagarTM Staphylococcus aureus and CHROMagarTM MRSA) were used for characterizing S. aureus and MRSA, cefoxitin disk diffusion method were also performed, vancomycin sensitivity by disk diffusion method and minimum inhibition concentration were evaluated using fluorescent microscope and subculture. 38.46% (20/52) were S. aureus identified on mannitol salt agar and on CHROMagarTM Staphylococcus aureus detected 19/52 (36.5%), 17 isolates of them were MRSA detected by CHROMagarTM MRSA and cefoxitin disk diffusion method, the vancomycin MICs value were 1.25µg/ ml of 64.71% and 1.75µg/ml of 35.29% . nebulizers applying by many different patients therefore can play a vital role in controlling on infection within a hospital, MRSA are associate with these infections detecting with significant numbers and resistant to many antibiotics, phenotypic detection like; chromogenic agars and cefoxitin deck diffusion method are highly recommended for rabid detection, vancomycin has high effective on MRSA and the best drug for treating bacterial resistance infections.


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