Pharmacological correction of changes in the chemical and mineral composition of rat’s skeletal bones by Mexidol after 60-day of Tartrazine administration

Abstract

Currently, the yellow synthetic azo dye tartrazine is widely used in such industrial fields as pharmy, food and cosmetology. The effects of tartrazine on morphological and functional state of bones as well as ways of correction of changes insufficiently studied. The aim of the study was to establish the possibilities of using Mexidol as a pharmacological corrector for changes in the chemical and mineral composition of skeletal bones in mature rats after 60 days of using tartrazine. The research was carried out on 175 white mature male rats, divided into 5 groups. 1st group - control; 2nd and 3rd groups - 1 ml of tartrazine solution was administered to rats daily intragastrically at dose 750 and 1500 mg/kg body weight respectively for 60 days; 4th and 5th groups - 1 ml of tartrazine solution was administered to rats daily intragastrically at dose 750 and 1500 mg/kg body weight respectively for 60 days and intramuscularly 5% solution of mexidol at dose 50 mg/kg body weight. The periods of observation were 3, 10, 15, 24 and 45 days after the end of 60-days tartrazine administration. The chemical and mineral composition of the humerus, hip bones, and the third lumbar vertebra were studied by gravimetric method. Also the spectrophotometry was used. The application of mexidol is accompanied by smoothing out the negative effect of 60-day tartrazine administration at dose of 7500 and 1500 mg/kg body weight on the mineral and chemical composition of the rat’s skeletal bones from the 15th to the 45th days of observation. The mineral and chemical structure of bones was recovered to control values more quickly in group with the use of tartrazine at dose of 750 mg/kg body weight.

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