Pecularities of the influence of cyclophosphamide and imunophan on the processes of apoptosis and necrosis in Peyer’s patches of rats’ small intestine
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 2, pp. 803-806
  • Published Online: 07 Jul 2019
  • Article Views: 318 | Article Download: 152
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AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Morozova EN, Morozov VN, Tverskoi AV, Zabolotnaya SV, Kaliuzhnaya EV. Pecularities of the influence of cyclophosphamide and imunophan on the processes of apoptosis and necrosis in Peyer’s patches of rats’ small intestine. Eurasia J Biosci. 2019;13(2), 803-806.

APA 6th edition
In-text citation: (Morozova et al., 2019)
Reference: Morozova, E. N., Morozov, V. N., Tverskoi, A. V., Zabolotnaya, S. V., & Kaliuzhnaya, E. V. (2019). Pecularities of the influence of cyclophosphamide and imunophan on the processes of apoptosis and necrosis in Peyer’s patches of rats’ small intestine. Eurasian Journal of Biosciences, 13(2), 803-806.

Chicago
In-text citation: (Morozova et al., 2019)
Reference: Morozova, Elena N., Vitaliy N. Morozov, Alexey V. Tverskoi, Svetlana V. Zabolotnaya, and Elena V. Kaliuzhnaya. "Pecularities of the influence of cyclophosphamide and imunophan on the processes of apoptosis and necrosis in Peyer’s patches of rats’ small intestine". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 803-806.

Harvard
In-text citation: (Morozova et al., 2019)
Reference: Morozova, E. N., Morozov, V. N., Tverskoi, A. V., Zabolotnaya, S. V., and Kaliuzhnaya, E. V. (2019). Pecularities of the influence of cyclophosphamide and imunophan on the processes of apoptosis and necrosis in Peyer’s patches of rats’ small intestine. Eurasian Journal of Biosciences, 13(2), pp. 803-806.

MLA
In-text citation: (Morozova et al., 2019)
Reference: Morozova, Elena N. et al. "Pecularities of the influence of cyclophosphamide and imunophan on the processes of apoptosis and necrosis in Peyer’s patches of rats’ small intestine". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 803-806.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Morozova EN, Morozov VN, Tverskoi AV, Zabolotnaya SV, Kaliuzhnaya EV. Pecularities of the influence of cyclophosphamide and imunophan on the processes of apoptosis and necrosis in Peyer’s patches of rats’ small intestine. Eurasia J Biosci. 2019;13(2):803-6.

Abstract

In this article, we studied the peculiarities of cyclophosphamide and imunofan effect on the processes of apoptosis and necrosis in Peyer’s patches of the rats’ small intestine. The study was conducted on 18 white mature rats, which were divided into three groups. Group I was administered imunofan according to the scheme on days 1, 3, 5, 7, 9 of the experiment at a dose of 0.7 µg/kg of body weight of the animal, Group II was administered cyclophosphamide once at a dose of 200 mg/kg (for 1 day), then imunofan according to scheme (on the 2nd, 4th, 6th, 8th, 10th day of the experiment) at a dose of 0.7 µg/kg body weight of the animal, and Group III - intact animals served. Ultrathin sections of Peyer’s patches of the rats’ small intestine were prepared and studied under electron microscope with further photographing. Mostly one type of cell death - apoptosis is detected in group I animals on the 7th and 30th day of the experiment in Peyer’s patches. The number of cells with apoptosis is directly proportional to the observation period, which seems to indicate the ability of imunofan to influence this process, maintaining the constancy of Peyer’s patches cellular composition in small intestine. In animals of group II, on the 7th day of the experiment, both apoptosis and necrosis take place, and on the 30th day it is mainly apoptosis. Apparently, this is due to the immunosuppressive effect of cyclophosphamide on the lymphocytes of Peyer’s patches of the small intestine, which manifests itself in the early period of the experiment after correction by the immunomodulator.

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