Over expression of p16ink4a associated with high grade lesions of (CIN) and cervical cancer (study applied on women infected with HPV in Basrah, Iraq)

Abstract

CIN lesions and cervix cancer is a consequence for the infection with HPV, molecular interaction between the virus and cellular proteins leads to an increase in expression of p16INK4a and inhibitor for CDK, so p16INK4a expression is a biomarker for HR-HPV. CIN grading and SCC according to p16INK4a expression pattern and the associated infection with HR-HPV was analysed in. Biopsy samples were collected from 52 women’s cervix with an age ranged from (15) to (>75) years exhibited abnormal histopathological changes for period between 1/2/2018 to 1/2/2019 from Basrah Hospital for women and children in Basrah, Iraq. The HPV prevalence detection was carried out by PCR using the general Primer My09/My11. cDNA s from the same samples were used as templates for gene expression analysis of E6, E7 mRNA in HR-HPV types using qPCR. The same samples used also to evaluate the P16INK4a expression by IHC. The PCR results showed 45 (86.54%) samples were infected with HPV with highest detection about (36%) in age group (35-45) years and 7 (13.46%) samples were negative to HPV. Gene expression of oncogenes E6 & E7 showed high percintage for HPV-16 (36%). Immunohistochemistry staining showed an increase in p16INK4a expression and intensity staining in the high grade of CIN and carcinoma and (P< 0.001). in our study, p16INK4a shows overexpression for HR-HPV infected cases with high grade of CIN and carcinoma.

Keywords

HPV P16INK4a CIN

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