Eurasian Journal of Biosciences

Non-thermal carbon dioxide-mediated inactivation process of Escherichia coli using bespoke system
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 4821-4827
  • Published Online: 31 Oct 2020
  • Open Access Full Text (PDF)


The reduction of the number of contaminated microbial cells in the biological and food solutions has desirably led to the development of many inactivation technologies aimed at the same goal--eradication of the microbial cells without harming the biological solution or generating toxic chemical agents. E.coli is known as food borne pathogen and cause serious problems in food industry and is used as a model organism in this study. The current CO2 mediated approach was used as a cheap and emerging approach to inactivate bacterial cells, E. coli. CO2 mediated approach is a non-thermal method, which has advantages over traditional methods such as autoclaving and γ -radiation. The results show that injecting CO2 into the system reduced the bacterial population by ~ 2.5-Log after 90 min and to 3.6 Log reduction after lactic acid addition. Bacterial cells were suffered from morphological changes and shape changes were obvious. Efficient energy consumption, avoidance of hazardous substances usage and can be applied in situ, are among its advantages, making it a promising and emerging inactivation technology.


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