N-acetyl-Cysteine effects on tissue malondialdehyde level and tissue edema on the Ischemia-Eperfusion injury of white rats’ (Rattus Norvegicus) skeletal muscles

Abstract

Background: N-acetylcysteine ​​(NAC), as an antioxidant, has been proven to have protective effects on cells undergoing oxidative stress. In the case of skeletal muscles, NAC can reduce muscle fatigue by decreasing Na+ K+ pump activity during exercise, and improve skeletal muscle microcirculation in an experimental model of severe closed soft-tissue injury. Purpose: This study aims to determine NAC effects on the ischemia-reperfusion injury of skeletal muscles by measuring tissue malondialdehyde (MDA) levels and edema, as well as the effect of NAC dose on MDA levels. Method: Randomization was carried out to 30 white rats (Rattus norvegicus), which then divided into 3 treatment groups, i.e., K group as the control group (NaCl 0.9%), PTX (Pentoxyfilline) group, as well as P1, P2, and P3 treatment groups. ANOVA test was employed to identify the differences in MDA levels and water content among groups. Results: ANOVA test results indicated that there was a significant difference between the control group (K) and the treatment group given NAC (P1, 2, 3), p = 0.007, while there was no difference in edema indicating that there were no different data groups among the five treatment groups with a p-value = 0.616 (p> 0.05). Conclusion: NAC administration provides positive results by reducing tissue MDA levels with the recommended dose of 200 mg/kg of body weight. NAC administration does not reduce tissue edema, and there are no significant differences with the Pentoxifylline group.

References

  • Blaisdell, F. W. (2002). The pathophysiology of skeletal muscle ischemia and the reperfusion syndrome: a review. Cardiovascular surgery, 10(6), 620-630.
  • Collard, C. D., & Gelman, S. (2001). Pathophysiology, clinical manifestations, and prevention of ischemia-reperfusion injury. Anesthesiology: The Journal of the American Society of Anesthesiologists, 94(6), 1133-1138.
  • Cuzzocrea, S., Mazzon, E., Costantino, G., Serraino, I., Dugo, L., Calabrò, G.,... & Caputi, A. P. (2000). Beneficial effects of n‐acetylcysteine on ischaemic brain injury. British journal of pharmacology, 130(6), 1219-1226.
  • Dorweiler, B., Pruefer, D., Andrasi, T. B., Maksan, S. M., Schmiedt, W., Neufang, A., & Vahl, C. F. (2007). Ischemia-reperfusion injury. European Journal of Trauma and Emergency Surgery, 33(6), 600-612.
  • Inci, I., Zhai, W., Arni, S., Hillinger, S., Vogt, P., & Weder, W. (2007). N-acetylcysteine attenuates lung ischemia–reperfusion injury after lung transplantation. The Annals of thoracic surgery, 84(1), 240-246.
  • Jegatheeswaran, S., & Siriwardena, A. K. (2011). Experimental and clinical evidence for modification of hepatic ischaemia–reperfusion injury by N-acetylcysteine during major liver surgery. HPB, 13(2), 71-78.
  • Katzung, B. G. (2012). Basic and clinical pharmacology. Mc Graw Hill.
  • Kearns, S. R., Moneley, D., Murray, P., Kelly, C., & Daly, A. F. (2001). Oral vitamin C attenuates acute ischaemia-reperfusion injury in skeletal muscle. The Journal of bone and joint surgery. British volume, 83(8), 1202-1206.
  • Kearns, S. R., O’Briain, D. E., Sheehan, K. M., Kelly, C., & Bouchier-Hayes, D. (2010). N-acetylcysteine protects striated muscle in a model of compartment syndrome. Clinical Orthopaedics and Related Research®, 468(8), 2251-2259.
  • Kerksick, C., & Willoughby, D. (2005). The antioxidant role of glutathione and N-acetyl-cysteine supplements and exercise-induced oxidative stress. Journal of the international society of sports nutrition, 2(2), 38.
  • Lukiswanto, B. S., Yuniarti, W. M., & Motulo, Y. Y. (2017). Effects of hyperbaric therapy on liver morphofunctional of rabbits (Oryctolagus cuniculus) after hind limb ischemia-reperfusion injury. Veterinary World, 10(11), 1337.
  • McArdle, A., Pattwell, D., Vasilaki, A., Griffiths, R. D., & Jackson, M. J. (2001). Contractile activity-induced oxidative stress: cellular origin and adaptive responses. American Journal of Physiology-Cell Physiology, 280(3), C621-C627.
  • McKenna, M. J., Medved, I., Goodman, C. A., Brown, M. J., Bjorksten, A. R., Murphy, K. T.,... & Gong, X. (2006). N‐acetylcysteine attenuates the decline in muscle Na+, K+‐pump activity and delays fatigue during prolonged exercise in humans. The Journal of physiology, 576(1), 279-288.
  • Norgren, L., Hiatt, W. R., Dormandy, J. A., Nehler, M. R., Harris, K. A., & Fowkes, F. G. R. (2007). Inter-society consensus for the management of peripheral arterial disease (TASC II). Journal of vascular surgery, 45(1), S5-S67.
  • Orhan, G., Yapici, N., Yuksel, M., Sargin, M., Şenay, Ş., Yalçin, A. S.,... & Aka, S. A. (2006). Effects of N-acetylcysteine on myocardial ischemia–reperfusion injury in bypass surgery. Heart and vessels, 21(1), 42-47.
  • Sahin, S., Karabey, Y., Kaynak, M. S., & Hincal, A. A. (2006). Potential use of freeze-drying technique for estimation of tissue water content. Methods and findings in experimental and clinical pharmacology, 28(4), 211-216.
  • Schaser, K. D., Bail, H. J., Schewior, L., Stover, J. F., Melcher, I., Haas, N. P., & Mittlmeier, T. (2005). Acute effects of N‐acetylcysteine on skeletal muscle microcirculation following closed soft tissue trauma in rats. Journal of orthopaedic research, 23(1), 231-241.
  • Simelane, M. M., Masuku, M. B., Rugambisa, J. I., & Earnshaw, D. M. (2016). Analysis of Consumer Willingness to Pay for Genetically Modified Maize Food in Swaziland. International Journal of Sustainable Agricultural Research, 3(1), 19-28.
  • WANG, H. G., LI, Z. Y., & LIU, X. L. (2006). Addition of tanshinone IIA to UW solution decreases skeletal muscle ischemia‐reperfusion injury 1. Acta Pharmacologica Sinica, 27(8), 991-999.
  • Ward, R., Souder, N., Stahl, D., Hunter, F., Probe, R., Chaput, C., & Childs, E. (2009). The role of nitric oxide synthase and heme oxygenase in the protective effect of hypothermia in ischemia-reperfusion injury. JBJS, 91(11), 2637-2645.

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