Molecular study on Cytolysins, Adhesins, Siderophores and Efflux pump among Cervico-Vaginal Escherichia coli
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2019 - Volume 13 Issue 2, pp. 1849-1857
  • Published Online: 02 Dec 2019
  • Article Views: 371 | Article Download: 217
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AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Al-Khaqani MM, Al-Dahmoshi HOM, Alwash MS. Molecular study on Cytolysins, Adhesins, Siderophores and Efflux pump among Cervico-Vaginal Escherichia coli. Eurasia J Biosci. 2019;13(2), 1849-1857.

APA 6th edition
In-text citation: (Al-Khaqani et al., 2019)
Reference: Al-Khaqani, M. M., Al-Dahmoshi, H. O. M., & Alwash, M. S. (2019). Molecular study on Cytolysins, Adhesins, Siderophores and Efflux pump among Cervico-Vaginal Escherichia coli. Eurasian Journal of Biosciences, 13(2), 1849-1857.

Chicago
In-text citation: (Al-Khaqani et al., 2019)
Reference: Al-Khaqani, Marwa M., Hussein O. M. Al-Dahmoshi, and Mourouge S. Alwash. "Molecular study on Cytolysins, Adhesins, Siderophores and Efflux pump among Cervico-Vaginal Escherichia coli". Eurasian Journal of Biosciences 2019 13 no. 2 (2019): 1849-1857.

Harvard
In-text citation: (Al-Khaqani et al., 2019)
Reference: Al-Khaqani, M. M., Al-Dahmoshi, H. O. M., and Alwash, M. S. (2019). Molecular study on Cytolysins, Adhesins, Siderophores and Efflux pump among Cervico-Vaginal Escherichia coli. Eurasian Journal of Biosciences, 13(2), pp. 1849-1857.

MLA
In-text citation: (Al-Khaqani et al., 2019)
Reference: Al-Khaqani, Marwa M. et al. "Molecular study on Cytolysins, Adhesins, Siderophores and Efflux pump among Cervico-Vaginal Escherichia coli". Eurasian Journal of Biosciences, vol. 13, no. 2, 2019, pp. 1849-1857.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Al-Khaqani MM, Al-Dahmoshi HOM, Alwash MS. Molecular study on Cytolysins, Adhesins, Siderophores and Efflux pump among Cervico-Vaginal Escherichia coli. Eurasia J Biosci. 2019;13(2):1849-57.

Abstract

Background: Bacterial vaginosis is an important female reproductive tract infection. It push a threat of miscarriage or fertility-related problems if it untreated. Cervico-vaginal E. coli (CVEC) is the common cause agent of microbial vaginosis. It equipped by a set of adhesins, immune-disguise protein, cytolysins, siderophores and metal efflux pump that enable them from establishing and endure the infection.
Methodology: One hundred fifty seven (157) vaginal swabs were collected from female clinically diagnosed with bacterial vaginitis. The swab was put in tubes of broth of Brain heart infusion (BHIB) to keep them vital and non-dry until being taken to the laboratory and the inoculated on MacConkey agar Eosin methylene blue agar. The only pinky colony with green-metallic shine, on MacConkey agar Eosin methylene blue agar respectively, were selected for study. Polymerase chain reaction using specific primer pairs was used to investigate the virulence factors at molecular level.
Results: The results revealed that, out of (157) clinical samples only 32 (20.38%) isolates were belonged to E. coli. Regarding the adhesins the results revealed that FimH adhesin was the most common virulence factor and was discovered in 25( 78.13%) of CVEC strains, followed by FocG 23(71.87% ), PapGII 22(68.75%), GafD 12(37.5%) and SfaS 8(21.87%) while PapGI and PapGIII not detected in the current study. Concern the cytolysins the results showed that 25(78.13%), 27(84.38%) of CVEC isolates possess hemolysin gene (hlyA) and phospholipase A gene (pldA). The investigation of both kpsMT-K1 and kpsMT-K5 genes. The results revealed that kpsMT-K1 were predominant and compile 10(31.25%), while kpsMT-K5 (which encodes for K5 capsule) were not detected. Aerobactin gene (iucD) were detected in 24(75%) of CVEC isolates. The presence of cusC gene (as a representative for cusCFBA operon responsible for copper/silver efflux) revealed that all CVEC have cusC 32(100%).
Conclusion: The current study conclude possession of CVEC for a sets of virulence effectors that enable them from establish the infection, dissemination, immune system evading and sustainability of infection.

References

  • Abdi HA, Rashki A, Rashki Z, Shahkarami F, Shahraki Z (2014) The relationship between phylogenetic group and distribution of virulence genes hlyA, iroN, iucD, fimH in Escherichia coli isolated from female genital tract among women attending gynecology clinics in Zabol-Iran by Multiplex-PCR. Iranian Journal of Medical Microbiology, 7(4): 9-15.
  • Africa CWJ, Nel J, Stemmet M (2014) Anaerobes and bacterial vaginosis in pregnancy: Virulence factors contributing to vaginal colonization. International Journal of Environmental Research and Public Health, 11(7): 6979-7000. https://doi.org/10.3390/ijerph110706979
  • Al-Dahmoshi HO, Al-Mamoori AM, Al-Khafaji NS (2016) Investigation of CusCFBA pump among uropathogenic Escherichia coli (UPEC) isolate from women with cystitis, Iraq. Biosciences Biotechnology Research Asia, 14(1): 1-5. https://doi.org/10.13005/bbra/2420
  • Al-Mayahie SMG (2014) Phylogenetic grouping and virulence characterization of ESBL-Producing and non-producing vaginal Escherichia coli. Science Publishing Group, 2(5): 127-133. https://doi.org/10.11648/j.ajbls.20140205.14
  • Amin ET, Njumkeng C, Kika BT, Fualefac A, Njukeng P (2018) Pattern of Antimicrobial Resistance among Bacterial Isolates from Urogenital Clinical Specimens: A Descriptive Study from the Buea Health District, Cameroon. Drugs-real world outcomes, 5(2): 101-108. https://doi.org/10.1007/s40801-018-0132-2
  • Bien J, Sokolova O, Bozko P (2012) Role of Uropathogenic Escherichia coli virulence factors in development of urinary tract infection and kidney damage. International Journal of Nephrology, 2012(2012): 1-15. https://doi.org/10.1155/2012/681473
  • Birosová E, Siegfried L, Kmet’ová M, Makara A, Ostró A, Gresová A, et al. (2004) Detection of virulence factors in alpha-haemolytic Escherichia coli strains isolated from various clinical materials. Journal of Clinical Microbiology, 10(6): 569-573. https://doi.org/10.1111/j.1469-0691.2004.00922.x
  • Chen M, Tofighi R, Bao W, Aspevall O, Jahnukainen T, Gustafsson LE, Ceccatelli S, Celsi G (2006) Carbon monoxide prevents apoptosis induced by uropathogenic Escherichia coli toxins. Pediatric Nephrology (Berlin, Germany), 21(3): 382-389. https://doi.org/10.1007/s00467-005-2140-1
  • Cook SW, Hammill HA, Hull RA (2001) Virulence factors of Escherichia coli isolated from female reproductive tract infections and neonatal sepsis. Infectious Diseases in Obstetrics and Gynecology, 9(4): 203-207. https://doi.org/10.1155/S1064744901000333
  • Devi U, Barman N, Barua P, Malik V, Das JK, Baruah P, Mahanta J (2014) Vaginal carriage of antibiotic resistant Escherichia coli by pregnant women: A concern for the Neonate. Clinical Microbiology: Open Access, 3(4): 153. https://doi.org/10.4172/2327-5073.1000153
  • Esmaeili D, Saghi H, Bahador A (2014) Study of Inhibition of Apha6 Gene in Acinetobacter Baumanii by Satureja and Thyme Essence with Rt-Pcr Technique. The International Journal of Biotechnology, 3(9): 115-122.
  • Ewers C, Antao EM, Diehl I, Philipp HC, Wieler LH (2009) Intestine and environment of the chicken as reservoirs for extraintestinal pathogenic Escherichia coli strains with zoonotic potential. Applied and Environmental Microbiology, 75(1): 184-192. https://doi.org/10.1128/AEM.01324-08
  • Farnam J, Miri GR, Rezazade MH (2016) Satisfaction Assessment of the Quality of Life of Urban Families and their Roles in the Development of Zabol. International Journal of Geography and Geology, 5(2): 26-33. https://doi.org/10.18488/journal.10/2016.5.2/10.2.26.33
  • Franke S, Grass G, Rensing C, Nies DH (2003) Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli. Journal of Bacteriology, 185(13): 3804-3812. https://doi.org/10.1128/JB.185.13.3804-3812.2003
  • Gao Q, Wang X, Xu H, Xu Y, Ling J, Zhang D, Gao S, Liu X (2012) Roles of iron acquisition systems in virulence of extraintestinal pathogenic Escherichia coli: Salmochelin and aerobactin contribute more to virulence than heme in a chicken infection model. BMC Microbiology, 12(1): 143. https://doi.org/10.1186/1471-2180-12-143
  • Ghanbarpour R, Salehi M (2010) Determination of Adhesin Encoding genes in Escherichia coli isolates from Omphalitis of chicks. American Journal of Animal and Veterinary Sciences, 5(2): 91-96. https://doi.org/10.3844/ajavsp.2010.91.96
  • Guiral E, Bosch J, Vila J, Soto SM (2010) Prevalence of Escherichia coli among samples collected from the genital tract in pregnant and nonpregnant women: Relationship with virulence. FEMS Microbiology Letters, 314(2): 170-173. https://doi.org/10.1111/j.1574-6968.2010.02160.x
  • Hilbert DW, Paulish TE, Mordechai E, Adelson ME, Trama JP (2008) O serogroups, phylogeny, and virulence factors of cervico vaginal and rectal Escherichia coli isolates. European Journal of Clinical Microbiology and Infectious Diseases, 27(12): 1265-1268. https://doi.org/10.1007/s10096-008-0574-7
  • Jain S, Nagarjuna D, Gaind R, Chopra S, Debata PK, Dawar R, Sardana R, Yadav M (2016) Escherichia vulneris: An unusual cause of complicated diarrhoea and sepsis in an infant. A case report and review of literature. New Microbes New Infect. 13: 83-86. https://doi.org/10.1016/j.nmni.2016.07.002
  • Johnson JR, O’Bryan TT (2004) Detection of the Escherichia coli group 2 Polysaccharide capsule synthesis gene kpsM by a rapid and specific PCR-Based assay. Journal of Clinical Microbiology, 42(4): 1773-1776. https://doi.org/10.1128/JCM.42.4.1773-1776.2004
  • Johnson JR, Stell AL (2000) Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. The Journal of Infectious Diseases, 181(1): 261-272. https://doi.org/10.1086/315217
  • Kaczmarek A, Budzynska A, Gospodarek E (2012) Prevalence of genes encoding virulence factors among Escherichia coli with K1 antigen and non-k1 E. coli strains. Journal of Medical Microbiology, 61(10): 1360-1365. https://doi.org/10.1099/jmm.0.044263-0
  • Kim KS (2001) Escherichia coli translocation at the blood-brain barrier. Infection and Immunity, 69(9): 5217-5222. https://doi.org/10.1128/IAI.69.9.5217-5222.2001
  • Kim KS (2003) Pathogenesis of bacterial meningitis: from bacteremia to neuronal injury. Nature Reviews Neuroscience, 4: 376-385. https://doi.org/10.1038/nrn1103
  • Lamont RF, Sobel JD, Akins RA, Hassan SS, Chaiworapongsa T, Kusanovic JP, Romero R (2011) The vaginal microbiome: New information about genital tract flora using molecular based techniques. An International Journal of Obstetrics and Gynaecology, 118(5): 533-549. https://doi.org/10.1111/j.1471-0528.2010.02840.x
  • Lasaro MA, Salinger N, Zhang J, Wang Y, Zhong Z, Goulian M, Zhu J (2009) F1C fimbriae play an important role in biofilm formation and intestinal colonization by the Escherichia coli commensal strain Nissle 1917. Applied and Environmental Microbiology, 75(1): 246-251. https://doi.org/10.1128/AEM.01144-08
  • Lorenz TC (2012) Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies. JoVE (Journal of Visualized Experiments), (63): e3998. https://doi.org/10.3791/3998
  • Márquez-López A, Del R, Cano M, Rodríguez-Mirones C, Oteo J, Sáez, D, Martínez-Martínez L (2013) Production of HlyA and ClyA haemolysins among quinolone-resistant Escherichia coli isolated from clinical samples. Springer Plus, 2(1): 2-71. https://doi.org/10.1186/2193-1801-2-71
  • Melican K, Sandoval RM, Kader A, Josefsson L, Tanner GA, Molitoris BA, Richter-Dahlfors A (2011) Uropathogenic Escherichia coli P and type 1 fimbriae act in synergy in a living host to facilitate renal colonization leading to nephron obstruction. PLoS Pathogens, 7(2). https://doi.org/10.1371/journal.ppat.1001298
  • Mendz GL, Kaakoush NO, Quinlivan JA (2013) Bacterial aetiological agents of intra-amniotic infections and preterm birth in pregnant women. Frontiers in Cellular and Infection Microbiology, 3: 58. https://doi.org/10.1371/journal.ppat.1001298
  • Nwogwugwu NU, Dozie INS, Chinakwe EC (2015) The incidence of asymptomatic urinary tract infections amongst female university students in Owerri-South East Nigeria. Annals of Biological Research, 6(10): 20-25.
  • Obata-Yasuoka M, Ba-Thein W, Tsukamoto T, Yoshikawa H, Hayashi H (2002) Vaginal Escherichia coli share common virulence factor profiles, serotypes and phylogeny with other extraintestinal E. coli. Microbiology (Reading, England), 148(9): 2745-2752. https://doi.org/10.1099/00221287-148-9-2745
  • Qabajah MH (2011) Avian Pathogenic Escherichia coli (APEC) in Palestine: Characterization of Virulence Factors and Antibiotic Resistance Profile. A thesis.
  • Rahman H, Deka M (2014) Detection & characterization of necrotoxin producing Escherichia coli (NTEC) from patients with urinary tract infection (UTI). Indian Journal of Medical Research, 139(4): 632-637.
  • Rani S, Mehra R, Gupta V, Huria A, Chander J (2014) Vaginal flora in preterm premature rupture of membranes and their sensitivity to commonly used antibiotics. Asian Journal of Medical Science, 5(4). https://doi.org/10.3126/ajms.v5i4.9889
  • Rashki A (2014) Cervico-vaginopathogenic Escherichia coli in Iran: Serogroup distributions, virulence factors and antimicrobial resistance properties. Microbial Pathogenesis, 75: 29-34. https://doi.org/10.1016/j.micpath.2014.08.004
  • Sáez-López E, Guiral E, Fernández-Orth D, Villanueva S, Goncé A, López M, et al. (2016) Vaginal versus Obstetric infection Escherichia coli isolates among pregnant women: Antimicrobial resistance and genetic virulence profile. PLoS One, 11(1): 0146531. https://doi.org/10.1371/journal.pone.0146531
  • Saidenberg AB, Guedes NMR, Seixas GHF, Allgayer MDC, de Assis EP, Silveira LF, Melville PA, Benites NR (2012) A Survey for Escherichia coli virulence factors in asymptomatic Free-Ranging Parrots. ISRN Veterinary Science, 2012(2012): 6. https://doi.org/10.5402/2012/984813
  • Saier MH, Tran CV, Barabote RD (2006) TCDB: The transporter classification database for membrane transport protein analyses and information. Nucleic Acids Research, 34: 181-186. https://doi.org/10.1093/nar/gkj001
  • Sambrook JF, Russell DW (2001) Molecular Cloning: A Laboratory Manual. 1, 2, 3: 2100. 3 th Edition.
  • Surgers L, Bleibtreu A, Burdet C, Clermont O, Laouénan C, Lefort A, et al. (2014) Escherichia coli bacteraemia in pregnant women is life-threatening for fetuses. Clinical Microbiology and Infection, 20(12): 1035-1041. https://doi.org/10.1111/1469-0691.12742
  • Tamelienė R, Barčaitė E, Stonienė D, Buinauskienė J, Markūnienė E, Kudrevičienė A, Vitkauskienė A, Jomantienė D, Nadišauskienė R (2012) Escherichia coli colonization in neonates: Prevalence, perinatal transmission, antimicrobial susceptibility, and risk factors. Medicina (Kaunas, Lithuania), 48(2): 71-76. https://doi.org/10.3390/medicina48020013
  • Van de Wijgert JHHM, Borgdorff H, Verhelst R, Crucitti T, Francis S, Verstraelen H, Jespers V (2014) The vaginal microbiota: What have we learned after a decade of molecular characterization?. PLOS ONE, 9(8): 105998. https://doi.org/10.1371/journal.pone.0105998
  • Villar HE, Aubert V, Baserni MN, Jugo MB (2013) Maternal carriage of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli isolates in Argentina. Journal of Chemotherapy (Florence, Italy), 25(6): 324-327. https://doi.org/10.1179/1973947813Y.0000000081
  • Watt S, Lanotte P, Mereghetti L, Moulin-Schouleur M, Picard B, Quentin R (2003) Escherichia coli strains from pregnant women and neonates: intraspecies genetic distribution and prevalence of virulence factors. Journal of Clinical Microbiology, 41(5): 1929-1935. https://doi.org/10.1128/JCM.41.5.1929-1935.2003
  • Wullt B, Bergsten G, Connell H, Röllano P, Gebretsadik N, Hull R, Svanborg C (2000) P fimbriae enhance the early establishment of Escherichia coli in the human urinary tract. Molecular Microbiology, 38(3): 456-464. https://doi.org/10.1046/j.1365-2958.2000.02165.x
  • Yamamoto S, Terai A, Yuri K, Kurazono H, Takeda Y, Yoshida O (1995) Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction. FEMS Immunology and Medical Microbiology Journal, 12: 85-90. https://doi.org/10.1111/j.1574-695X.1995.tb00179.x

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