Molecular study of Rota A viruses at many farm animals in Iraq
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 3051-3057
  • Published Online: 17 Sep 2020
  • Open Access Full Text (PDF)

Abstract

Background: The species A of Rotavirus is considered as of medical and veterinary important pathogen, worldwide distributed and responsible for acute gastroenteritis particularly at juvenile ages, the viruses explain marked genetic diversity which represented by numerous number genotypes infect different vertebrate hosts. 
Methods: About 942 fecal samples had been taken directly from the rectum of 403 cattle, 236 sheep,174 goats and 129 of camels, their age younger than 6 months and suffering from diarrhea at different Iraqi Governorates; advanced specific molecular technique was implemented, Reverse Transcription -modern- Real Time PCR technique (rRT- PCR) with utilizing of a new generation thermo-cycler to obtain reliable result regarding exact viral genotypes in these animals at Iraq. 
Results: The RVAs were observed in 29.61% of diarrheic animals the percentages of infection among these animals as followings: cattle, sheep, goats and camels were 31.51%, 29.23%, 27.58% and 27.13%, respectively, the distribution pattern of viral genotypes as following: in cattle: G6, G8, G10, P[1] and P[5] in addition to P[11]; in sheep: G3, G6, G10, P[1] and P[11]; in goats: G6 and also P[5]; in camels: G10, P[1] and P[11]. G10 was the most dominant among specific genotypes in all animals in this study.
Conclusions: According to the author’s knowledge, the present study is the first study that recorded G3, G6, G8, G10, and also P [1], P [5] besides to P [11] in above animals at Iraq, which offers a valuable and reliable epidemiological information pertaining RAVs genotypes that were urgently needed for both human and animal necessary hygiene regards.

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