Molecular investigation of an unusual bacteria causing severe UTI among public water closet users: Mechanisms of characterization and Engineering solutions

Abstract

Urinary tract infections (UTIs) compose one of the most common community-acquired infections, where Escherichia vulneris does not signify a pathogenic role in it. Nevertheless, recent studies indicate that insufficient water closets play a vital role in the implications of disease transmission and health problems for all attendees. Besides, the defective water closets at the educational facilities with insufficient access to clean toilets could bring harmful health effects such as severe urinary tract infections for both staff members and students due to their exposure to bioaerosol infections which are transmitted easily in dirty public water closets. Thus, this study designed to assess the prevalence of urinary tract infections among defined employees with the estimation of the role of individual water closets in the incidence of microbial etiology.
The Results show significant prevalence of UTIs recorded among employees of both sexes working at an educational facility and sharing the same unisex water closets during their daily-worktime. The causative pathogen isolated from both positive UTI urine samples and water closets was E.vulneris profiled by API 20E, identified by 16s rRNA gene sequence and the production of heat-labile and hemolysin toxins molecularly characterized by RT-PCR. The water closets indoor air quality and performance evaluated with sensors to plot the link of the UTI microbial etiology. 
From this study can be Concluded a Significantly prevalent upper and lower UTI cases encountered amongst employees of an educational facility where Escherichia vulneris was the main invading pathogen. Isolated strains of E.vulneris revealed a broad spectrum of antibiotic resistance and susceptibility to Cephalosporins and Ciprofloxacin only, the gene sequence of 1400bp of the 16s rRNA, and the production of hemolysin (hlyA 1177bp), and heat-labile (LT 508 bp) toxins. Besides, the inadequacy of the water closet at the workplace imposes an extra burden on the health of the employees as it might promote many community-acquired diseases.

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