Molecular detection of staphopain genes (A&B) from staphylococcus aureus isolated from skin lesions
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 4393-4396
  • Published Online: 26 Oct 2020
  • Open Access Full Text (PDF)

Abstract

In this study, A total of 100 clinical swabs obtained from patients with skin lesion and also from healthy individuals from aneroid extend from September to December 2019.
Only 20 isolates were identified and diagnosed by using chemical and cultural features as staphylococcus aureus where 13 isolates obtained from patients and 7 isolates from healthy skin. Molecular study by using PCR was done to show the presence of positive genes, ScpA, SspB, which encodes for staphopain A and staphopain B respectively. It was found that 13 isolates of S aureus (65%) give positive results for the existence of ScpA gene which encodes staphopain A whereas 7 isolates (35%) are free from this gene. Besides, 10 isolates of S. aureus (50%) gave positive results for SspB gene which encodes staphopain B.
All the 10 isolates containing SspB gene have the ScpA gene at the same times. Moreover, two genes associated with skin disease are also investigated; these genes are edin-B gene and etd gene. PCR technique by using specific primers for both genes found that only 10 isolates (50%) possessed edin-B gene whereas 15 isolates of S.aureus (75%) had etd gene.

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