Molecular detection of staphopain genes (A&B) from staphylococcus aureus isolated from skin lesions
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 4393-4396
  • Published Online: 26 Oct 2020
  • Open Access Full Text (PDF)


In this study, A total of 100 clinical swabs obtained from patients with skin lesion and also from healthy individuals from aneroid extend from September to December 2019.
Only 20 isolates were identified and diagnosed by using chemical and cultural features as staphylococcus aureus where 13 isolates obtained from patients and 7 isolates from healthy skin. Molecular study by using PCR was done to show the presence of positive genes, ScpA, SspB, which encodes for staphopain A and staphopain B respectively. It was found that 13 isolates of S aureus (65%) give positive results for the existence of ScpA gene which encodes staphopain A whereas 7 isolates (35%) are free from this gene. Besides, 10 isolates of S. aureus (50%) gave positive results for SspB gene which encodes staphopain B.
All the 10 isolates containing SspB gene have the ScpA gene at the same times. Moreover, two genes associated with skin disease are also investigated; these genes are edin-B gene and etd gene. PCR technique by using specific primers for both genes found that only 10 isolates (50%) possessed edin-B gene whereas 15 isolates of S.aureus (75%) had etd gene.


  • Ajayi A A, Oniha M I, Atolagbe O M, Onibokun E A, (2017). Studies on Staphylococcus aureus isolated from pimples. Pakistan Journal of Biological Sciences: 20,7, 350-354.‏
  • Al-Kelaby K K, AL-Kaabi K T, AL-Hilli E S (2016). Prevalence of Staphylococcus aureus in Atopic Dermatitis (Eczema) cases in Al-Najaf City/Iraq. 6(2), 168-176.
  • Ansari S, Jha R K, Mishra S K, Tiwari B R, Asaad A M (2019). Recent advances in Staphylococcus aureus infection: focus on vaccine development. Infection and drug resistance, 12, 1243.‏
  • Astley R, Miller F C, Mursalin M H, Coburn P S, Callegan M C (2019). An eye on Staphylococcus aureus toxins: roles in ocular damage and inflammation. Toxins, 11(6), 356.‏
  • Ataei Moghadam, Sh., Rostami Charati, F., Akbari, F., Gholamalipour Alamdari, E., Behmanesh, B. (202). Consideration antimicrobial and antioxidant properties of anbarnesa smoke ointment. Journal of Medicinal and Chemical Sciences, 3(3) 245-253.
  • Åvall-Jääskeläinen S, Taponen S, Kant R, Paulin L, Blom J, Palva A, Koort J (2018). Comparative genome analysis of 24 bovine-associated Staphylococcus isolates with special focus on the putative virulence genes. PeerJ, 6, e4560.‏
  • Dhulfiqar A, Muhamed S, Lames A (2015). Molecular Detection of Enterotoxin A and B and Biofilm Gene of Staphylococcus aureus Isolated from Nasal Carriers. Medical Journal of Babylon, 12(1), 1-7.
  • Gimza B D, Larias M I, Budny B G, Shaw L N (2019). Mapping the Global Network of Extracellular Protease Regulation in Staphylococcus aureus. mSphere, 4(5), e00676-19.‏
  • Guo Y, Song G, Sun M, Wang J, Wang Y (2020). Prevalence and Therapies of Antibiotic-Resistance in Staphylococcus aureus. Frontiers in Cellular and Infection Microbiology, 10, 107.‏
  • Hume E B, Cole N, Khan S, Walsh B J, Willcox M D (2020). The role of staphopain a in Staphylococcus aureus keratitis. Experimental Eye Research, 107994.‏
  • Kareem A, Thikra A and Hala A (2016). Study of methicillin resistant S.aureus isolated from different hospital in najaf city - iraq, J.of medical science of medical research vol 4 (8) 2016 11922-11930.
  • Kobayashi T, Nakaminami H, Ohtani H, Yamada K, Nasu Y, Takadama S,Matsumoto T (2020). An outbreak of severe infectious diseases caused by methicillin-resistant Staphylococcus aureus USA300 clone among hospitalized patients and nursing staff in a tertiary care university hospital. Journal of Infection and Chemotherapy, 26(1), 76-81.‏
  • Lehman M K, Nuxoll A S, Yamada K J, Kielian T, Carson S D, Fey P D (2019). Protease-mediated growth of Staphylococcus aureus on host proteins is opp3 dependent. MBio, 10(2).‏
  • Linda M, McDonald L C, Mandal S, & Jernigan D B (2006). Staphylococcus aureus–associated skin and soft tissue infections in ambulatory care. Emerging infectious diseases, 12(11), 1715.‏
  • Lindsay S, Oates A, & Bourdillon K (2017). The detrimental impact of extracellular bacterial proteases on wound healing. International wound journal, 14(6), 1237-1247.‏
  • Magro G, Rebolini M, Beretta D, & Piccinini R (2018). Methicillin-resistant Staphylococcus aureus CC22-MRSA-IV as an agent of dairy cow intramammary infections. Veterinary microbiology, 227, 29-33.‏
  • Miyata E, Satoh S, Inokuchi K, Aso A, Kimura Y, Yokoyama S, Kishihara Y (2007). Three Fatal Cases of Rapidly Progressive Infective Endocarditis Caused by Staphylococcus Aureus. Circulation Journal, 71(9), 1488-1491.‏
  • Nasser A, Moradi M, Jazireian P, Safari H, Alizadeh-Sani M, Pourmand M R, Azimi T (2019). Staphylococcus aureus versus neutrophil: Scrutiny of ancient combat. Microbial pathogenesis, 131, 259-269.‏
  • Sakr A, Brégeon F, Mège J L, Rolain J M, Blin O, (2018). Staphylococcus aureus nasal colonization: an update on mechanisms, epidemiology, risk factors, and subsequent infections. Frontiers in microbiology, 9, 2419.‏
  • Tam K, Torres V J, (2019). Staphylococcus aureus secreted toxins and extracellular enzymes. Gram‐Positive Pathogens, 640-668.‏
  • Yamaguchi T, Nishifuji K, Sasaki M, Fudaba Y, Aepfelbacher M, Takata T, Sugai M (2002). Identification of the Staphylococcus aureus etd pathogenicity island which encodes a novel exfoliative toxin, ETD, and EDIN-B. Infection and immunity, 70(10), 5835-5845.‏


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