Enterococcal bacteria have emerged as main nosocomial pathogens. Virulence factor is important in enhancing ability to cause disease. Some of which are very significant in the pathogenesis of diseases caused via them. Resistance genes only does not indicate pathogenicity of bacteria, presence them with virulence factors it can cause the strain to be dangerous. Eighty-nine Enterococci were recovered from patient with cystitis. All isolates confirmed by m-EI chromogenic agar and the virulence factors were assayed by conventional PCR. It was found that esp observed in (35.4%) E.fecalis, (30%) E.faecium and (36.7%, 10%) E.faecalis and E.faecium respectively have asa1 gene, (22.7%) E.fecalis give positive result and all E.faecium was free of ace, (8.8%) E.faecalis have cyl gene and all of E. faecium didn’t have this gene, 6.3% of E.faecalis and no one of E.faecium have hyl gene. 16.4% of E.faecalis and 30% have EF3314 gene. virulence factors commonly prevalent in enterococcus isolated in this study, the incidence of virulence factors was higher average number in E.faecalis and much lower average number in E. faecium.

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