Molecular detection of some tetracycline-resistant genes in Staphylococcus aureus isolated from sheep milk in Al-Qadisiyah province of Iraq
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 1, pp. 1161-1166
  • Published Online: 28 May 2020
  • Article Views: 98 | Article Download: 95
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Esmael GK, Majeed NM. Molecular detection of some tetracycline-resistant genes in Staphylococcus aureus isolated from sheep milk in Al-Qadisiyah province of Iraq. Eurasia J Biosci. 2020;14(1), 1161-1166.

APA 6th edition
In-text citation: (Esmael & Majeed, 2020)
Reference: Esmael, G. K., & Majeed, N. M. (2020). Molecular detection of some tetracycline-resistant genes in Staphylococcus aureus isolated from sheep milk in Al-Qadisiyah province of Iraq. Eurasian Journal of Biosciences, 14(1), 1161-1166.

Chicago
In-text citation: (Esmael and Majeed, 2020)
Reference: Esmael, Ghassan Khudhair, and Noor Mahmood Majeed. "Molecular detection of some tetracycline-resistant genes in Staphylococcus aureus isolated from sheep milk in Al-Qadisiyah province of Iraq". Eurasian Journal of Biosciences 2020 14 no. 1 (2020): 1161-1166.

Harvard
In-text citation: (Esmael and Majeed, 2020)
Reference: Esmael, G. K., and Majeed, N. M. (2020). Molecular detection of some tetracycline-resistant genes in Staphylococcus aureus isolated from sheep milk in Al-Qadisiyah province of Iraq. Eurasian Journal of Biosciences, 14(1), pp. 1161-1166.

MLA
In-text citation: (Esmael and Majeed, 2020)
Reference: Esmael, Ghassan Khudhair et al. "Molecular detection of some tetracycline-resistant genes in Staphylococcus aureus isolated from sheep milk in Al-Qadisiyah province of Iraq". Eurasian Journal of Biosciences, vol. 14, no. 1, 2020, pp. 1161-1166.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Esmael GK, Majeed NM. Molecular detection of some tetracycline-resistant genes in Staphylococcus aureus isolated from sheep milk in Al-Qadisiyah province of Iraq. Eurasia J Biosci. 2020;14(1):1161-6.

Abstract

Our study aims to investigate some tetracycline resistance genes in isolates of Staphylococcus aureus that collected from ewes milk that collected randomly. Sixty Milk samples are taken from ewes randomly and submitted to culturing on blood agar then submitted to PCR methods for final confirming detection of Staphylococcus aureus by using specific primers designed for (Glpf) gene depending on NCBI and Primer3 then making sensitivity test for tetracycline then make PCR for detection of some tetracycline resistance genes. Our results showed positive samples for Staphylococcus aureus was 14/60 (23.3%) by using PCR. Besides, all Staphylococcus aureus isolates were submitted to the tetracycline sensitivity test (disk diffusion method (Kirby‐Bauer) by tetracycline. Moreover, showing the percentage of tetracycline resistance isolates was 8/14(57.1%), and tetracycline sensitive isolates were 6/14 (42.8 %). Also, our results showed the percentage of tetracycline resistance genes tet(L) and tet(k) were 9/14 (64.2%) and 7/14 (50%) respectively, and percentage of isolates that carry gene tet(L) and tet(K) together was 2/14 (14.2)% by using polymerase chain reaction technique. Our study concluded ewes milk that contaminated by Staphylococcus aureus at a high rate, and the non-response to treatment by tetracycline is occurred due to present tetracycline resistance genes such as tet(L) and tet(k), were causes treatment failure by tetracycline.

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