Molecular detection of 16srrna gene in escherichia coli isolated from urinary tract infection patients
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 1, pp. 99-104
  • Published Online: 29 Feb 2020
  • Open Access Full Text (PDF)


The present study aimed to isolate and to diagnose of Uropathogenic Escherichia coli which caused (UPEC). From 75 isolation were collected from children whom caused UPEC during 2015. Fifty of total isolates diagnosis as E. coli and entirely were used to detect 16SrRNA gene by polymerase chain reaction (PCR) technique and DNA sequencing. Disc diffusion method was used to detect antibiotic sensitivity against of 8 antibiotics. The results of present study showed different in resistant percentage against Penicillin, Tetracycline, Amoxicillin, Nalidixic acid, Trimethoprim-Sulphamethoxazo, Nitrofuranation, Gentamycin, Nitrofuranation and Chloramphenicol. The resistant percentage of the isolates which: 50 (100%), 48 (96%), 47 (94.8%), 38 (76%), 30 (60%), 22 ‎‎(44%), 11 (22%), 9 (18%) respectively. The results of PCR showed all E. coli samples had the targeted gene and 28 samples recorded as new strains Wid which documented in NCBI.


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