Metformin effects on vaspin levels and other parameters in Iraqi polycystic ovarian women
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 4493-4501
  • Published Online: 26 Oct 2020
  • Open Access Full Text (PDF)

Abstract

Patients with polycystic ovarian syndrome showed hormonal imbalance and hyperandrogenemia because of higher secretion of androgen from theca cells of ovaries and adrenal gland due to excess LH or hyperinsulinemia. Obesity and increase fat mass aid the pathophysiology of PCOS, therefore, Different studies try to find the relationship of PCOS with Vaspin protein, which expressed in visceral adipose tissue resulting in increased insulin sensitivity by different mechanisms. This study is designed to show the effect of metformin treatment on Vaspin levels in newly diagnosed PCOS patients treated by this drug and the association of PCOS hormonal changes with Vaspin level
Forty three patients of newly diagnosed PCOS patients are included in the study with no abnormal thyroid or adrenal function, they do not use oral contraceptive or other treatment to treat their PCOS before the study, they agree to start metformin 850 mg twice daily for three months and provide fasting blood sample with measuring BMI, at the second day of menstruation before starting treatment and after completing three months of treatment. FSH, LH, SHBG, total testosterone, insulin, glucose, estradiol are determined by cobas technique, while Vaspin and free testosterone are determined by ELISA technique. Homeostatic model assessment is used to measure insulin resistance (HOMA-IR) is determined according to the following formula: HOMA-IR=(Fasting Insulin multiplied by Fasting glucose)divided by 22.5 
It was found that there were no significant changes in BMI, FSH,LH, SHBG, total testosterone, estradiol and vaspin after end of treatment for all patients, but free testosterone and HOMA-IR showed significant decrease at p-value 0.02 and 0.03 respectively. 
The study reveals that metformin can induce decrease in free testosterone and insulin resistance but cannot produce sever weight loss or changes in hormones regulating menstrual cycle, SHBG or Vaspin. The study suggested that there are different factors can affect Vaspin level in PCOS patients other than insulin resistance as its level failed to decrease after intake of insulin sensitizing drug, metformin.

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