Measurement of toxicity levels of cholera toxin on peripheral blood mononuclear cells (PBMCs)
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 1, pp. 2143-2145
  • Published Online: 30 Jun 2020
  • Open Access Full Text (PDF)

Abstract

Cholera toxin is an exotoxin responsible for pathogenicity of those bacteria targeting the immune system of a host represented by cellular immunity, the first defense line in the body. In this brief study, we illustrated the cellular effect of vibrio cholera toxin on the viability of immune cell (lymphocytes, monocytes, or macrophages) population together on the peripheral blood mononuclear cells (PBMCs). The result of trypan blue exclusion assay demonstrated the effect of dose and time on the virulence of cholera toxin, a dose- and time-dependent effect, thereby low doses produced the cytotoxic effect at long duration of exposure while high doses possess the strongest growth inhibition effect at short duration. In conclusion, the cholera toxin has a potential effect on the cells with different doses at different time points.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.