Background/aim: Cholesterol is recycled in the body. The liver excretes cholesterol into biliary fluids, which is then stored in the gallbladder, which then excretes it in a non-esterified form (via bile) into the digestive tract. Typically, about 50% of the excreted cholesterol is reabsorbed by the small intestine back into the bloodstream Cholesterol is one of several biological problems that can result in death, especially for high-risk groups, such as older people and those with hypertension and hypercholesterolemia, and can lead to atherosclerosis and reduce work productivity as well. Red algae extract (Kappaphycus alvarezii) affects the total cholesterol level because the content of β-carotene reduces the formation and oxidation from low-density lipoprotein proteins (LDL cholesterol). This study aimed to determine the effect of red algae extract (Kappa hycusalvarezii) administration with a dose of 140 mg/200grBW on the total cholesterol levels of Wistar strain male rats (Rattus norvegicus) induced by a high-fat diet. Materials and methods: This research used 30 rats that met inclusion requirements, and they were divided randomly into three groups: the normal group (K₀), feed group (K₁), and Kappaphycus alvarezii group (K₂). An examination of cholesterol levels was done for each group on the 31^st day. The sample was analyzed using the CHOD-PAP (Cholesterol oxidase para aminophenazone) method. The data were analyzed using a one-way ANOVA test. Results: Statistics based on Mann-Whitney U tests for significance levels between the group that had not been given and the other group that had been given the extract of red algae (Kappaphycus alvarezii) with a dose of 140 mg/200grBW for rats that had been given a high-fat diet were less than 0.05 (Sig<0.05), resulting in 0.001. Conclusions: Administering the extract of red algae (Kappaphycus alvarezii) with a dose of 140 mg/200grBW affected the total cholesterol level of Wistar strain male rats (Rattus norvegicus) inducted with a high-fat diet.

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