Objective: The present study was designed to test the level of some cardiac hormones like endothelin-1 and Atrial natriuretic peptide (ANP) in serum of heart diseases patients compare with control group. This study was conducted on heart diseases patients in Cardiac Care Unit (C.C.U.) in Salah- Aldin General Hospital in Tikrit City-Salah-Aldin governorate. The study, began in 31 Desember 2018 to 1 st September 2019 and included 90 subjects divided in two groups ,the first group was (10) apparently healthy subjects10 male and 10 female, while the second group was 80 patients with different heart diseases (20 Heart Failure (HF) 10 male and 10 female, 20 Myocardial Infarction (MI) 10 male and 10 female, 20 Unstable Angina (UA) 10 male and 10 female, 20 Stable Angina (SA) 10 male and 10 female, were attended from Salah - Aldin General Hospital. Blood samples was collected from patients and healthy subjects and then serum separated for testing. The results of this study showed there were a high significant increasing ( P≤ 0.01) in the concentration of endothelin-1 and Atrial natriuretic peptide (ANP) in all Heart disease groups as compared with control healthy subjects and the highest concentration of endothelin-1 was found in heart failure group, while the highest concentration of ANP was found in myocardial infarction group. Material and methods: Venous blood samples were collected from the vein from all patients and healthy subjects at study entry and after a 20-min period and serum was separated by centrifugation at 1500 g for 10 min at 4 C^◦ for hormonal testing. Endothelin-1and Atrial natriuretic peptide (ANP) hormones concentration in serum of heart diseases patients was estimated depends on kit procedure is an Enzyme-Linked Immunosorbent Assay (ELISA) from Bioassay Technology, China. Results: There was a high significant increasing ( P≤ 0.01) in the concentration of endothelin-1 in all heart disease groups as compared with control healthy subjects and the highest concentration of endothelin-1 was found in heart failure group, ANP There was a high significant increasing ( P≤ 0.01) in the concentration of ANP in all Heart disease groups as compared with control healthy subjects ,the highest concentration of (ANP ng/ml) was found in Myocardial infarction group. Conclusion: Serum endothelin-1 and Atrial natriuretic peptide (ANP) hormone concentration were significantly increased (P≤ 0.01) in all Heart diseases groups as compared with control healthy subjects. This finding suggests that endothelin may be a marker of rapid coronary artery disease progression and may also have a pathogenic role in this process.

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