Eurasian Journal of Biosciences

Isolation and diagnosis of Fusarium Solani that causes root rot soybean and evaluating the efficiency of bacteria Bacillus Subtilus and Azotobacter Spp in controlling the disease


This research was conducted with the aim of studying root rot disease and seedlings death in soybeans that causes by fungus Fusarium solani in some areas of wasit governorate, isolating the pathogen and obtaining six isolates of Fusarium solani isolates from the areas covered by this study and testing its pathogenic ability, evaluating the efficiency of the plant growth promoting Rhizobacteria (PGPR) Azotobacter spp and Bacillus subtilus isolates, in percentage germination of soybean seeds, reducing percentage of infection and severity of disease and its effect in the length of vegetative and root groups of soy bean plant and comparing the results with beltanol fungicides. The percentage of seed germination treated with Bacillus subtilis, Azotobacter spp and beltanol was 95, 95 and 90%, respectively, compared to the comparison treatment 1 (without any addition) that reached 82%. and compared to the comparison treatment 2 (pathogen only) 65%. The treatment of soybean plant with bacteria under study showed a reduction in the infection rate after the trial period, it reached 15 and 10%, respectively, compared to the comparison treatment that included the pathogenic fungus, the infection rate reached 85%.While the severity of the disease incidence in these two treatments was 6.6 and 5%, respectively, compared to the comparison treatment was 42.5%.The biological control and chemical pesticide treatment that used in this study was able to improvement the growth of the plant according to the results of some growth criteria of plant length, vegetative and root groups, and their soft weight.


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