Isolation and diagnosis of Fusarium Solani that causes root rot soybean and evaluating the efficiency of bacteria Bacillus Subtilus and Azotobacter Spp in controlling the disease

Abstract

This research was conducted with the aim of studying root rot disease and seedlings death in soybeans that causes by fungus Fusarium solani in some areas of wasit governorate, isolating the pathogen and obtaining six isolates of Fusarium solani isolates from the areas covered by this study and testing its pathogenic ability, evaluating the efficiency of the plant growth promoting Rhizobacteria (PGPR) Azotobacter spp and Bacillus subtilus isolates, in percentage germination of soybean seeds, reducing percentage of infection and severity of disease and its effect in the length of vegetative and root groups of soy bean plant and comparing the results with beltanol fungicides. The percentage of seed germination treated with Bacillus subtilis, Azotobacter spp and beltanol was 95, 95 and 90%, respectively, compared to the comparison treatment 1 (without any addition) that reached 82%. and compared to the comparison treatment 2 (pathogen only) 65%. The treatment of soybean plant with bacteria under study showed a reduction in the infection rate after the trial period, it reached 15 and 10%, respectively, compared to the comparison treatment that included the pathogenic fungus, the infection rate reached 85%.While the severity of the disease incidence in these two treatments was 6.6 and 5%, respectively, compared to the comparison treatment was 42.5%.The biological control and chemical pesticide treatment that used in this study was able to improvement the growth of the plant according to the results of some growth criteria of plant length, vegetative and root groups, and their soft weight.

References

  • Abdul-Mohsen R G, Nadhum T A, and Alhumairy Y N (2018).Isolating and Diagnosis The Fungi That Causes Rot Root Disease on Corn and Testing The Pathogenicity and Efficiency Evaluation Of Biological And Chemical Control. Wassit journal for science and medicine:2018.
  • AL-Ani R A, Adahab M A, Abood H M (2012).Rhizobium japanicum as biocontrol agent of soybean root rot diseases caused by Fusarium solani and Macrophomina phaseolinae.Plant protect.Sci.48:149-155
  • Alexopolus G J, Mims C W, andBlack M W (1996).mycology,4thEd,John.WandSons.r,New York,869pp
  • Al-Sahoki M M(1991) Soybeans Production and Improvement. Al-Hekma bulding for printing and publishing.University of Baghdad. 230
  • Bakker P A, Ran L X, Pieteres C M, and Vanloon L C (2003).Understanding the involvement of Rhizobacteria mediated induction of systemic resistance in biocontrol of plant disease.Can.J.Plant Patthol.25:5-9.
  • Bolkan H H, and Butler E E (1974). Studies on heterokaryosis virulence of Rhizoctonia solani. Phytophology. 64: 513-522.
  • Branawy M A S, Abdul-wahid O A (2006).Field resistance of crosses of (Sesamum indicum L.)to charcoal root rot caused by Macrophomina phaseolina.
  • Burges L W, Dodman R L, Pont W and Mayers P (1981).Fusarium Diseases of Wheat,Maize,Soybeans and grain in Nelon P E, Toussoun T A and Cook R G (Eds),Fusarium:Disease,biology and taxonomy.Pennsylvania State University Press,University Park, Pennsylvania,pp.64-76.
  • Burgess L W, and Summerell B A (1988).Laboratory manual for Fusarium research.2nded.Univ.Sydney,Australia,156p.
  • Chen L S, Figueredo A and Villani H (2002).Diversity and symbiotic effectiveness of rhizobia isolated from field-grown soybean nodules in Paraguay.Biol.Fertl.Soils 35:448-457.
  • De la vega L M, Barboza M G, and Ramirez M L (2006).Purification and characterization of an exochitinase from Bacillus thuringiensis and its action against phytophathogenic fungi. Canadian Journal of Microbiology. Vol.52 (7): 651-657.
  • Dewan M M (1989). Identity and frequency occurrence of fungi in roots of wheat and rye grass and their effect on take-all and host growth. Ph.D. thesis. Univ. of Western Australia. 201 pp.
  • Dimitrios B A and Anasatasiok K A, Evangelia S C, Constsantian R F (2017). Impact of a beneficial and of a pathogenic Fusarium strain on the fingerprinting-based structure of microbial communities in tomato (Lycopersicon esculentum Milll.) rhizosphere. World Journal of Agrciculture science 27:621-630.
  • Einallash H, Farshid A S, Ali B T (2014).The role of soil organisms on oil stability. International Journal of current life science vol.4.Issue,11,pp-10328-10334,November,2014.
  • EL-Batanony N H, et al (2007).The inhibitory effect culture filtrate as some wild Rhizobium spp on some faba bean root rot pathogen and their anti-microbial synergetic effect when combined with arbuscular mycorrhiza.
  • Friedman M and Brandon D (2001).Nutrition and health benefits of soy bean protein.Journal of Agriculture and food chemistry 49:1069-1068.
  • Gray L E, and Achenbach A (1996).Severity of foliar symptoms and root and crown rot of soybean inoculated with various isolates and inoculum rates of fusarium solani.Plant disease 80:7797-7799.
  • Hapgood F (1987).The prodigious soy bean.National Geografic 172:66-91.
  • Hasson K H, Kifa H R and Kadhum Z K (2013).Effect of Bacteria Bacillus subtilis,Bacillus thuringiensis andPseudomonas fluorescens in biological control of okra plant from the infection by Rhizoctonia solani fungus. Al Furat Journal of Agricultural Sciences 5(4)148- 164,(2013).
  • James J H, Assistant Professor and Extension Educator, Agriculture and Natural Resources (2016). Role of Soil Bacteria.Ohio state University.
  • Kleopper J WTuzun S L I and Wei G (1993).Plant growth promoting rhizobacteria as inducer of systemic disease resistance.Pages156-165.In:Pest Management Biologically Based Technologies Chemical Society Books. Washington,D.C.
  • Lesile J F and Summerell B A (2006).The Fusarium laboratory manual.blackwell publishing. Iowa,USA.388 PP.
  • Marasas W F O, Burges L W, Anelick R Y, Lamprecht S C, andVanSchalkwyk D J (1988).Survey of fusarium species associated with plant debries in south African soils.S.Afr.J.Bot.,54:63-71.
  • Mckinney H H (1923).Influence of soil temperature and moisture on infection of wheat seedling by Heliminthosporium sativum.
  • Nelson B D (1999).fusarium blight or wilt,root rot,and pods and collar rot.page 35-36 in Hartmen G L, Sinclair J B,and Rupe J C,eds.compendium of soy bean disease.4th ed APS,st.paul,MN.35PP.
  • Nelson B D,Hansen J M, and Helms T C (1997).Reaction of soy bean cultivators to isolates of Fusarium solani from the red river vally.Plant Dis-81:664-668.
  • Peix A, Mateos P E, Rodriguez-Barrueco C, Martinez-Molina E and Velazquez E (2001).Growth promotion of common bean (Phaseolus vulgaris) by strain of Burkholderia cepacia under growth chamber conditions.Soil Biol.Biochem.33(14):1927-1935.
  • Sapna C, Manjula V, Kanula W, and Neeru N (2012).Potential of Azotobacter spp as biocotrol against Rhizoctonia solani and Fusarium solani in cotton.Artical in Archives of Agronomy and soil science 58(12):1-12 December 2012.
  • Sherif M H (2013).Soybean,Nutrition and health. Puplished 20th 2013.DOI:10.577 2/5 54545.
  • Sing R S (1984).Introduction to principles of plant pathology.3rd ed Oxford.IBH publishing Co PVT.LTD.
  • Vigdis T (2002). Microbial diversity and function soil: from genes to ecosystems. Current Opinion in Microbiology. 5, 240–245. in
  • Wrather J A, and koening S R (2006).Estimates of diseases effect on soy bean yield in the United states from 2003-2005.J.Nematol.38:137-180.
  • Wrather J A, Stienstar W C and koening S R (2001).Soy bean disease loss estimates for the United states from 1996 to 1998.Can.J.Plant pathol.23:122-131.

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