Isolation and characterization of Staphylococcus haemolyticus from urinary tract infection

Abstract

Staphylococcus haemolyticus is a member of coagulase negative staphylococci colonize skin. The present study aimed to isolate and characterize S. haemolyticus from urinary tract infection (UTI). One hundred forty clinical samples isolated from UTI were included in this study. The colony morphology, gram stain and coagulase enzyme were tested against the typical colonies. All the selected isolated were subjected to Vitec 2 compact for identification and antibiotics sensitivity testing. The formation of biofilm was tested for all the isolates of S. haemolyticus. Biofilm formulation has been examined on S. haemolyticus isolates. The effect of different concentrations of carbohydrates, pH and NaCl on formation of biofilm was further analyzed. Among the collected samples, thirty were identified as S. haemolyticus. All tested strains of S. haemolyticus were resistant to antibiotics except two tigecycline and linezolid. All the isolates produced biofilm, one isolate was the highest with OD value 0.7. The biofilm formation was affected by the physio-chemical factors. S. haemolyticus isolated from UTI subjects. Multi resistant antibiotics were observed. Further studies are necessary to identify the anti-biofilm.

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